The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Ultrasensitive Immunosensor for Cancer Biomarker Proteins Using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification
Open accessAbstract
A densely packed gold nanoparticle platform combined with a multiple-enzyme labeled detection antibody-magnetic bead bioconjugate was used as the basis for an ultrasensitive electrochemical immunosensor to detect cancer biomarkers in serum. Sensitivity was greatly amplified by synthesizing magnetic bioconjugates particles containing 7500 horseradish peroxidase (HRP) labels along with detection antibodies (Ab2) attached to activated carboxyl groups on 1 microm diameter magnetic beads. These sensors had sensitivity of 31.5 microA mL ng(-1) and detection limit (DL) of 0.5 pg mL(-1) for prostate specific antigen (PSA) in 10 microL of undiluted serum. This represents an ultralow mass DL of 5 fg PSA, 8-fold better than a previously reported carbon nanotube (CNT) forest immunosensor featuring multiple labels on carbon nanotubes, and near or below the normal serum levels of most cancer biomarkers. Measurements of PSA in cell lysates and human serum of cancer patients gave excellent correlations with standard ELISA assays. These easily fabricated AuNP immunosensors show excellent promise for future fabrication of bioelectronic arrays.
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Magnetic nanoparticles in biomedical applications: A review
Materón, E. M., Miyazaki, C. M., Carr, O., Joshi, N., Picciani, P. H. S., Dalmaschio, C. J., Davis, F., & Shimizu, F. M. (2021) · Applied Surface Science Advances · doi:10.1016/j.apsadv.2021.100163 · Cited by 456
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Point of care testing: The impact of nanotechnology
Syedmoradi, L., Daneshpour, M., Alvandipour, M., Gomez, F. A., Hajghassem, H., & Omidfar, K. (2017) · Biosensors and Bioelectronics · doi:10.1016/j.bios.2016.08.084 · Cited by 367
Metadata
- Authors
- Mani, V., Chikkaveeraiah, B. V., Patel, V., Gutkind, J. S., & Rusling, J. F.
- Venue
- ACS Nano
- Publisher
- American Chemical Society (ACS)
- Published
- 2009-02-13
- Volume
- 3
- Issue
- 3
- Pages
- 585-594
- ISSN
- 1936-0851 (ISSN-L)
- 1936-086X
- OpenAlex
- W2058541035
- Cited by
- 532
- References
- 51
- FWCI
- 21.12
- Type
- journal article
- Language
- en
- Open access
- green
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- Mani, V., Chikkaveeraiah, B. V., Patel, V., Gutkind, J. S., & Rusling, J. F. (2009). Ultrasensitive Immunosensor for Cancer Biomarker Proteins Using Gold Nanoparticle Film Electrodes and Multienzyme-Particle Amplification. ACS Nano. https://doi.org/10.1021/nn800863w
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