Cited by 206Alzheimer's disease research and treatmentsMass Spectrometry Techniques and ApplicationsDementia and Cognitive Impairment Research

Determination of β-Amyloid Peptide Signatures in Cerebrospinal Fluid Using Immunoprecipitation-Mass Spectrometry

Portelius, Erik, Westman-Brinkmalm, Ann, Zetterberg, Henrik, Blennow, Kaj·2006·Journal of Proteome Research

Abstract

Early pathogenic events in Alzheimer's disease (AD) involve increased production and/or reduced clearance of beta-amyloid (Abeta), especially the 42 amino acid fragment Abeta1-42. The Abeta1-42 peptide is generated through cleavage of the amyloid precursor protein by beta- and gamma-secretase and is catabolised by a variety of proteolytic enzymes such as insulin-degrading enzyme and neprilysin. Here, we describe a method that employs immunoprecipitation combined with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry to determine the pattern of C-terminally truncated Abeta peptides in cerebrospinal fluid (CSF). Using antibodies coupled to magnetic beads, we have detected 18 C-terminally and 2 N-terminally truncated Abeta peptides in CSF. By determining the identity and profile of the truncated Abeta peptides, more insight may be gained about differences in the metabolism and structural properties of Abeta in AD. Finally, the Abeta fragment signatures may prove useful as a diagnostic test for AD.

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Crossref · 10.1021/pr050475v

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  • More than 20 additional amyloid-beta proteoforms have been detected in Alzheimer's brain samples from endogenous cleavage and post-translational modifications.

    “In addition to the widely known amyloid-beta 40 and amyloid-beta 42 peptides, over 20 additional amyloid-beta proteoforms have been detected in samples of Alzheimer’s brain samples arising from endogenous cleavage and post-translational modifications.(12,13)”

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Authors
Portelius, E., Westman-Brinkmalm, A., Zetterberg, H., & Blennow, K.
Affiliations
  • Sahlgrenska University Hospital (SE)
  • Institute of Clinical Neuroscience, Clinical Neurochemistry Laboratory, Sahlgrenska University Hospital, 43180 Mölndal, Sweden
Corresponding author
Portelius, Erik
Venue
Journal of Proteome Research
Published
2006-03-10
Volume
5
Issue
4
Pages
1010-1016
ISSN
  • 1535-3893 (ISSN-L)
  • 1535-3907
OpenAlex
W2089765958
Cited by
206
References
31
Citations by year
2026: 4 · 2025: 5 · 2024: 5 · 2023: 10 · 2022: 7 · 2021: 13 · 2020: 9 · 2019: 8 · 2018: 13 · 2017: 13 · 2016: 11 · 2015: 25 · 2014: 14 · 2013: 11 · 2012: 15
FWCI
5.32
Type
journal article
Language
en
Open access
closed
Locations
  • Journal of Proteome Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Medicine › Physiology › Alzheimer's disease research and treatments
  • Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
  • Health Sciences › Medicine › Psychiatry and Mental health › Dementia and Cognitive Impairment Research
Keywords
amyloid-beta, amyloid-beta 42, cerebrospinal fluid, Alzheimer's disease, amyloid precursor protein, immunoprecipitation, MALDI-TOF mass spectrometry, amyloid metabolism, diagnostic biomarkers
MeSH terms
Amino Acid Sequence, Humans, Models, Biological, Molecular Sequence Data, Molecular Weight, Peptides, Precipitin Tests, Sensitivity and Specificity, Mass Spectrometry, Amyloid beta-Peptides, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

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