Practical Points in Urinary Proteomics

Thongboonkerd, Visith

doi:10.1021/pr070328s

Cited by 208Advanced Proteomics Techniques and ApplicationsAdvanced Biosensing Techniques and ApplicationsMetabolomics and Mass Spectrometry Studies

Practical Points in Urinary Proteomics

Thongboonkerd, Visith·2007·Journal of Proteome Research

DOI 10.1021/pr070328s

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Abstract

During the proteomic era, one of the most rapidly growing areas in biomedical research is biomarker discovery, particularly using proteomic technologies. Urinary proteomics has become one of the most attractive subdisciplines in clinical proteomics, as the urine is an ideal source for the discovery of noninvasive biomarkers for human diseases. However, there are several barriers to the success of the field and urinary proteome analysis is not a simple task because the urine has low protein concentration, high levels of salts or other interfering compounds, and more importantly, high degree of variations (both intra-individual and inter-individual variabilities). This article provides step-by-step practical points to perform urinary proteome analysis, covering detailed information for study design, sample collection, sample storage, sample preparation, proteomic analysis, and data interpretation. The discussion herein should stimulate further discussion and refinement to develop guidelines and standardizations for urinary proteome study.

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Relative to blood, urine has a significantly narrower dynamic range of proteins, allowing more rapid preparation of specimens for proteomic interrogation.
“Relative to blood, urine evidences a significantly narrower dynamic range of proteins, thereby allowing more rapid preparation of specimens for proteomic interrogation.”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [105]
The urine proteome is relatively stable for up to six hours at room temperature and for over 17 years stored at −70°C.
“Additionally, the urine proteome is relatively stable for up to six hours at room temperature and for over 17 years stored at −70°C [105].”
From Update on biomarkers for the detection of endometriosis (2015) · cites this paper as [105]

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Update on biomarkers for the detection of endometriosis
Fassbender, A., Burney, R. O., F. O, D., D’Hooghe, T., & Giudice, L. (2015) · BioMed Research International · doi:10.1155/2015/130854 · Cited by 195 · see citing works
“Additionally, the urine proteome is relatively stable for up to six hours at room temperature and for over 17 years stored at −70C [105].”
Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments
Mischak, H., Coon, J. J., Novak, J., Weissinger, E. M., Schanstra, J. P., & Dominiczak, A. F. (2009) · Mass Spectrometry Reviews · doi:10.1002/mas.20205 · Cited by 193 · see citing works

Authors: Thongboonkerd, V.
Affiliations: Siriraj Hospital (TH)
Corresponding author: Thongboonkerd, Visith
ORCID: Thongboonkerd, Visith
Venue: Journal of Proteome Research
Publisher: American Chemical Society (ACS)
Published: 2007-09-07
Volume: 6
Issue: 10
Pages: 3881-3890
ISSN: 1535-3893 (ISSN-L)
1535-3907
DOI: 10.1021/pr070328s
OpenAlex: W2026884441
Cited by: 208
References: 45
Citations by year: 2026: 2 · 2025: 7 · 2024: 3 · 2023: 2 · 2022: 6 · 2021: 6 · 2020: 5 · 2019: 9 · 2018: 3 · 2017: 5 · 2016: 10 · 2015: 15 · 2014: 16 · 2013: 16 · 2012: 13
FWCI: 2.07
Type: journal article
Language: en
Open access: closed
Locations: Journal of Proteome Research (publishedVersion)
PubMed (publishedVersion)
Topics: Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Advanced Biosensing Techniques and Applications
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
Keywords: urine proteomics, urinary proteome, non-invasive biomarkers, biomarker discovery, clinical proteomics, urine collection, sample storage, sample preparation, research design, interindividual variability, standardization
MeSH terms: Humans, Proteinuria, Specimen Handling, Biomarkers, Proteome, Proteomics

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Practical Points in Urinary Proteomics

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