Capillary electrophoresis–mass spectrometry as a powerful tool in biomarker discovery and clinical diagnosis: An update of recent developments
Open accessAbstract
Proteome analysis has emerged as a powerful technology to decipher biological processes. One of the main goals is to discover biomarkers for diseases from tissues and body fluids. However, the complexity and wide dynamic range of protein expression present an enormous challenge to separation technologies and mass spectrometry (MS). In this review, we examine the limitations of proteomics, and aim towards the definition of the current key prerequisites. We focus on capillary electrophoresis coupled to mass spectrometry (CE–MS), because this technique continues to show great promise. We discuss CE–MS from an application point of view, and evaluate its merits and vices for biomarker discovery and clinical applications. Finally, we present several examples on the use of CE–MS to determine urinary biomarkers and implications for disease diagnosis, prognosis, and therapy evaluation. © 2008 Wiley Periodicals, Inc., Mass Spec Rev 28:703–724, 2009
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Capillary electrophoresis is a mature separation technology that couples effectively with mass spectrometry and is well suited for peptide separation.
“Capillary electrophoresis is a mature separation technology that is effectively coupled with mass spectrometry (CE-MS) and is well suited for this purpose7,8.”
From Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research (2019) · cites this paper as [7]
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Recent trends of capillary electrophoresis‐mass spectrometry in proteomics research
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CE‐MS‐based proteomics in biomarker discovery and clinical application
Pontillo, C., Filip, S., Borràs, D. M., Mullen, W., Vlahou, A., & Mischak, H. (2015) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201400115 · Cited by 77 · see citing works
Self-citationZhao, Y., Riley, N. M., Sun, L., Hebert, A. S., Yan, X., Westphall, M. S., Rush, M. J. P., Zhu, G., Champion, M. M., Medie, F. M., et al. (2015) · Analytical Chemistry · doi:10.1021/acs.analchem.5b00883 · Cited by 55 · see citing works
Self-citationKrenkova, J., & Foret, F. (2012) · PROTEOMICS · doi:10.1002/pmic.201200140 · Cited by 44 · see citing works
- Authors
- Mischak, H., Coon, J. J., Novak, J., Weissinger, E. M., Schanstra, J. P., & Dominiczak, A. F.
- Affiliations
- Mosaiques Diagnostics and Therapeutics (Germany) (DE)
- University of Wisconsin–Madison (US)
- Medizinische Hochschule Hannover (DE)
- Université Toulouse III - Paul Sabatier (FR)
- University of Glasgow (GB)
- Corresponding author
- Mischak, Harald
- Venue
- Mass Spectrometry Reviews
- Publisher
- Wiley
- Published
- 2008-10-30
- Volume
- 28
- Issue
- 5
- Pages
- 703-724
- ISSN
- 0277-7037 (ISSN-L)
- 1098-2787
- PMC
- PMC2720435
- OpenAlex
- W2137274797
- Cited by
- 193
- References
- 98
- Citations by year
- 2026: 5 · 2025: 1 · 2024: 4 · 2023: 4 · 2022: 2 · 2021: 8 · 2020: 4 · 2019: 9 · 2018: 11 · 2017: 7 · 2016: 9 · 2015: 20 · 2014: 13 · 2013: 22 · 2012: 20
- FWCI
- 1.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Mass Spectrometry Reviews (publishedVersion)
- PubMed (publishedVersion)
- Europe PMC (PubMed Central) (acceptedVersion)
- PubMed Central (acceptedVersion)
- ENLIGHTEN (Jurnal Bimbingan dan Konseling Islam) (submittedVersion)
- Funders
- Wellcome Trust (066780, 066780/2/012)
- British Heart Foundation (BHF RG/07/005/23633)
- National Institute of General Medical Sciences (R01GM080148)
- National Institute of Diabetes and Digestive and Kidney Diseases (R56DK078244, DK080301, DK061525, R24DK064400, R21DK080301, P01DK061525, DK064400, DK071802, R01DK071802, R01DK078244, DK078244)
- Topics
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Physical Sciences › Engineering › Biomedical Engineering › Microfluidic and Capillary Electrophoresis Applications
- Physical Sciences › Chemistry › Spectroscopy › Mass Spectrometry Techniques and Applications
- Keywords
- capillary electrophoresis-mass spectrometry, biomarker discovery, diagnosis, proteomics, urinary biomarkers, protein expression profiling, disease diagnosis, prognosis, treatment evaluation
- MeSH terms
- Humans, Proteins, Mass Spectrometry, Biomarkers, Electrophoresis, Capillary, Diagnostic Techniques and Procedures, Proteome
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