1H NMR-based Metabolomic Profiling in Mice Infected with Mycobacterium tuberculosis
Open accessAbstract
Tuberculosis (TB) is one of three major infectious diseases, and the control of TB is becoming more difficult because of the emergence of multidrug-resistant and extensively drug-resistant strains. In this study, we explored the (1)H NMR-based metabolomics of TB using an aerobic TB infection model. Global profiling was applied to characterize the responses of C57Bl/6 mice to an aerobic infection with virulent Mycobacterium tuberculosis (MTB). The metabolic changes in organs (i.e., the lung, the target organ of TB, and the spleen and liver, remote systemic organs) and in serum from control and MTB-infected rats were investigated to clarify the host-pathogen interactions in MTB-infected host systems. Principal components analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA) score plots showed distinct separation between control and MTB-infected rats for all tissue and serum samples. Several tissue and serum metabolites were changed in MTB-infected rats, as compared to control rats. The precursors of membrane phospholipids, phosphocholine, and phosphoethanolamine, as well as glycolysis, amino acid metabolism, nucleotide metabolism, and the antioxidative stress response were altered based on the presence of MTB infection. This study suggests that NMR-based global metabolite profiling of organ tissues and serum could provide insight into the metabolic changes in host infected aerobically with virulent Mycobacterium tuberculosis.
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“Then in 2011, three groups described mammalian itaconate production in three separate immune-related contexts: in the lungs of Mycobacterium tuberculosis (MTB)-infectedmice (Shin et al., 2011), in the supernatant of LPS-treated macrophage-like RAW264.”
Background
- Authors
- Shin, J.-H., Yang, J.-Y., Jeon, B.-Y., Yoon, Y. J., Cho, S.-N., Kang, Y.-H., Ryu, D. H., & Hwang, G.-S.
- Affiliations
- Division of Bacterial Respiratory Infection, Center for Infectious Diseases, National Institute of Health, Centers for Disease Control and Prevention, Seoul 122-701, Republic of Korea
- Chungnam National University (KR)
- Yonsei University (KR)
- Korea Basic Science Institute (KR)
- Sungkyunkwan University (KR)
- Corresponding author
- Ryu, Do Hyun
- Hwang, Geum-Sook
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2011-04-14
- Volume
- 10
- Issue
- 5
- Pages
- 2238-2247
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2090707513
- Cited by
- 247
- References
- 52
- Citations by year
- 2026: 13 · 2025: 15 · 2024: 28 · 2023: 11 · 2022: 11 · 2021: 21 · 2020: 29 · 2019: 24 · 2018: 15 · 2017: 11 · 2016: 14 · 2015: 16 · 2014: 15 · 2013: 12 · 2012: 7
- FWCI
- 8.41
- Type
- journal article
- Language
- en
- Open access
- Licence
- cc-by-nc-nd
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- YUHSpace (Yonsei University Medical Library) (submittedVersion, cc-by-nc-nd)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Metabolomics and Mass Spectrometry Studies
- Health Sciences › Medicine › Surgery › Infectious Diseases and Tuberculosis
- Keywords
- tuberculosis, Mycobacterium tuberculosis, host-pathogen interaction, metabolite profiling, mouse infection model, serum metabolites, phospholipid precursors, phosphorylcholine, phosphoethanolamine, glycolysis, amino acid metabolism, nucleotide metabolism, oxidative stress response, multidrug-resistant tuberculosis
- MeSH terms
- Animals, Female, Lung, Mycobacterium tuberculosis, Magnetic Resonance Spectroscopy, Tuberculosis, Discriminant Analysis, Principal Component Analysis, Mice, Rats, Host-Pathogen Interactions, Metabolomics, Metabolome
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