Confounding Effect of Obstructive Jaundice in the Interpretation of Proteomic Plasma Profiling Data for Pancreatic Cancer
Abstract
It is well established that variation in sampling, processing and storage protocols can alter the levels of potential biomarkers in serum and plasma. Here, using pancreatic cancer as an example, we demonstrate that consideration of clinical parameters related to the patient's illness is equally important when seeking cancer-specific biomarkers. Bile duct-obstruction is a feature of pancreatic disease that can cause jaundice. Comparing patients with pancreatic cancer, chronic pancreatitis or biliary duct obstruction, we observed that the plasma levels of apolipoprotein A1, transthyretin, and apolipoprotein E, when examined in isolation, were each associated with pancreatic cancer. However, when the effect of bile duct obstruction was considered, only transthyretin levels were independently associated with cancer likelihood. Our results demonstrate the importance of accounting for disease-related confounding factors when analyzing data for the detection of cancer biomarkers.
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Awareness is increasing that obstructive jaundice can lead to false-positive biomarker findings.
“Moreover, awareness that the presence of obstructive jaundice can lead to false-positive biomarker findings is increasing.69–71”
From Early detection of pancreatic cancer (2020) · cites this paper as [69]
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Self-citationBiomarkers for early diagnosis of pancreatic cancer
Jenkinson, C., Earl, J., Ghaneh, P., Halloran, C., Carrato, A., Greenhalf, W., Neoptolemos, J., & Costello, E. (2015) · Expert Review of Gastroenterology & Hepatology · doi:10.1586/17474124.2015.965145 · Cited by 42 · see citing works
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- Authors
- Yan, L., Tonack, S., Smith, R., Dodd, S., Jenkins, R. E., Kitteringham, N., Greenhalf, W., Ghaneh, P., Neoptolemos, J. P., & Costello, E.
- Affiliations
- University of Liverpool (GB)
- Corresponding author
- Costello, Eithne
- ORCID
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2008-12-04
- Volume
- 8
- Issue
- 1
- Pages
- 142-148
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W2053883425
- Cited by
- 44
- References
- 0
- Citations by year
- 2026: 1 · 2023: 3 · 2022: 2 · 2021: 2 · 2020: 3 · 2018: 1 · 2017: 3 · 2016: 1 · 2015: 3 · 2014: 4 · 2013: 2 · 2012: 3
- FWCI
- 2.79
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Proteome Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › Pancreatic and Hepatic Oncology Research
- Physical Sciences › Chemistry › Spectroscopy › Advanced Proteomics Techniques and Applications
- Health Sciences › Medicine › Surgery › Pancreatitis Pathology and Treatment
- Keywords
- pancreatic cancer, obstructive jaundice, biliary obstruction, plasma biomarkers, confounding factors, proteomic profiling, apolipoprotein A-I, prealbumin, apolipoprotein E, chronic pancreatitis, cancer detection
- MeSH terms
- Aged, Aged, 80 and over, Cholestasis, Female, Humans, Male, Middle Aged, Pancreatic Neoplasms, ROC Curve, Biomarkers, Tumor, Electrophoresis, Gel, Two-Dimensional, CA-19-9 Antigen, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Proteomics, Jaundice, Obstructive
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