The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Proteomic Analysis of Tumor Necrosis Factor-α-Induced Secretome of Human Adipose Tissue-Derived Mesenchymal Stem Cells
Abstract
Human adipose tissue-derived mesenchymal stem cells (hASCs) are useful for regeneration of inflamed or injured tissues. To identify secreted hASC proteins during inflammation, hASCs were exposed to tumor necrosis factor-alpha (TNF-alpha) and conditioned media derived from hASCs were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified 187 individual proteins as secreted proteins (secretome) in hASC-conditioned media; 118 proteins were secreted at higher levels upon TNF-alpha treatment. The TNF-alpha-induced secretome included a variety of cytokines and chemokines such as interleukin-6 (IL-6), IL-8, chemokine (C-X-C motif) ligand 6, and monocyte chemotactic protein-1 (MCP-1). TNF-alpha also increased expression of various proteases including cathepsin L, matrix metalloproteases and protease inhibitors, and induced secretion of long pentraxin 3, a key inflammatory mediator implicated in innate immunity. TNF-alpha-conditioned media stimulated migration of human monocytes, which play a key role in inflammatory responses. This migration was abrogated by pretreatment with neutralizing anti-IL-6, anti-IL-8, and anti-MCP-1 antibodies, suggesting that IL-6, IL-8, and MCP-1 are involved in migration of monocytes. Taken together, these results suggest that TNF-alpha-induced secretome may play a pivotal role in inflammatory responses and that shotgun proteomic analysis will be useful for elucidation of the paracrine functions of mesenchymal stem cells.
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Elias, J. E., & Gygi, S. P. (2007) · Nature Methods · doi:10.1038/nmeth1019 · Cited by 3,925
Mesenchymal stem cells as trophic mediators
Caplan, A. I., & Dennis, J. E. (2006) · Journal of Cellular Biochemistry · doi:10.1002/jcb.20886 · Cited by 2,992
Adipose-derived stem cells for regenerative medicine
Gimble, J. M., Katz, A. J., & Bunnell, B. A. (2007) · Circulation Research · doi:10.1161/01.res.0000265074.83288.09 · Cited by 2,332
Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells
Rehman, J., Traktuev, D., Li, J., Merfeld-Clauss, S., Temm-Grove, C. J., Bovenkerk, J. E., Pell, C. L., Johnstone, B. H., Considine, R. V., & March, K. L. (2004) · Circulation · doi:10.1161/01.cir.0000121425.42966.f1 · Cited by 2,269
Chen, L., Tredget, E. E., Wu, P. Y. G., & Wu, Y. (2008) · PLoS ONE · doi:10.1371/journal.pone.0001886 · Cited by 1,610
Cited by
Mesenchymal stromal cell secretome: influencing therapeutic potential by cellular pre-conditioning
Ferreira, J. R., Teixeira, G. Q., Santos, S. G., Barbosa, M. A., Almeida-Porada, G., & Gonçalves, R. M. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02837 · Cited by 572
Toh, W. S., Lai, R. C., Hui, J. H. P., & Lim, S. K. (2017) · Seminars in Cell & Developmental Biology · doi:10.1016/j.semcdb.2016.11.008 · Cited by 502
Mazini, L., Rochette, L., Amine, M., & Malka, G. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20102523 · Cited by 445
Adipokines: A treasure trove for the discovery of biomarkers for metabolic disorders
Lehr, S., Hartwig, S., & Sell, H. (2012) · PROTEOMICS – Clinical Applications · doi:10.1002/prca.201100052 · Cited by 352
Therapeutic Applications of Mesenchymal Stromal Cells: Paracrine Effects and Potential Improvements
Doorn, J., Moll, G., Le Blanc, K., van Blitterswijk, C., & de Boer, J. (2012) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2011.0488 · Cited by 307
Metadata
- Authors
- Lee, M. J., Kim, J., Kim, M. Y., Bae, Y.-S., Ryu, S. H., Lee, T. G., & Kim, J. H.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-03-12
- Volume
- 9
- Issue
- 4
- Pages
- 1754-1762
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1975822477
- Cited by
- 209
- References
- 5
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
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