The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
Proteomic Analysis of Tumor Necrosis Factor-α-Induced Secretome of Human Adipose Tissue-Derived Mesenchymal Stem Cells
Abstract
Human adipose tissue-derived mesenchymal stem cells (hASCs) are useful for regeneration of inflamed or injured tissues. To identify secreted hASC proteins during inflammation, hASCs were exposed to tumor necrosis factor-alpha (TNF-alpha) and conditioned media derived from hASCs were analyzed by liquid chromatography coupled with tandem mass spectrometry. We identified 187 individual proteins as secreted proteins (secretome) in hASC-conditioned media; 118 proteins were secreted at higher levels upon TNF-alpha treatment. The TNF-alpha-induced secretome included a variety of cytokines and chemokines such as interleukin-6 (IL-6), IL-8, chemokine (C-X-C motif) ligand 6, and monocyte chemotactic protein-1 (MCP-1). TNF-alpha also increased expression of various proteases including cathepsin L, matrix metalloproteases and protease inhibitors, and induced secretion of long pentraxin 3, a key inflammatory mediator implicated in innate immunity. TNF-alpha-conditioned media stimulated migration of human monocytes, which play a key role in inflammatory responses. This migration was abrogated by pretreatment with neutralizing anti-IL-6, anti-IL-8, and anti-MCP-1 antibodies, suggesting that IL-6, IL-8, and MCP-1 are involved in migration of monocytes. Taken together, these results suggest that TNF-alpha-induced secretome may play a pivotal role in inflammatory responses and that shotgun proteomic analysis will be useful for elucidation of the paracrine functions of mesenchymal stem cells.
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Cites
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Mesenchymal stem cells as trophic mediators
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Secretion of angiogenic and antiapoptotic factors by human adipose stromal cells
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Cited by
Mesenchymal stromal cell secretome: influencing therapeutic potential by cellular pre-conditioning
Ferreira, J. R., Teixeira, G. Q., Santos, S. G., Barbosa, M. A., Almeida-Porada, G., & Gonçalves, R. M. (2018) · Frontiers in Immunology · doi:10.3389/fimmu.2018.02837 · Cited by 572
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Adipokines: A treasure trove for the discovery of biomarkers for metabolic disorders
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Therapeutic Applications of Mesenchymal Stromal Cells: Paracrine Effects and Potential Improvements
Doorn, J., Moll, G., Le Blanc, K., van Blitterswijk, C., & de Boer, J. (2012) · Tissue Engineering Part B: Reviews · doi:10.1089/ten.teb.2011.0488 · Cited by 307
Review of the adipose derived stem cell secretome
Kapur, S. K., & Katz, A. J. (2013) · Biochimie · doi:10.1016/j.biochi.2013.06.001 · Cited by 305
Stem cells as drug delivery methods: Application of stem cell secretome for regeneration
Tran, C., & Damaser, M. S. (2015) · Advanced Drug Delivery Reviews · doi:10.1016/j.addr.2014.10.007 · Cited by 278
Mesenchymal stem cell-derived exosomes: a new therapeutic approach to osteoarthritis?
Mianehsaz, E., Mirzaei, H. R., Mahjoubin-Tehran, M., Rezaee, A., Sahebnasagh, R., Pourhanifeh, M. H., Mirzaei, H., & Hamblin, M. R. (2019) · Stem Cell Research & Therapy · doi:10.1186/s13287-019-1445-0 · Cited by 274
Kwon, Y. W., Heo, S. C., Jeong, G. O., Yoon, J. W., Mo, W. M., Lee, M. J., Jang, I.-H., Kwon, S. M., Lee, J. S., & Kim, J. H. (2013) · Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease · doi:10.1016/j.bbadis.2013.08.002 · Cited by 137
Cell secretome: basic insights and therapeutic opportunities for CNS disorders
Pinho, A. G., Cibrão, J. R., Silva, N. A., Monteiro, S., & Salgado, A. J. (2020) · Pharmaceuticals · doi:10.3390/ph13020031 · Cited by 94
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Metadata
- Authors
- Lee, M. J., Kim, J., Kim, M. Y., Bae, Y.-S., Ryu, S. H., Lee, T. G., & Kim, J. H.
- Venue
- Journal of Proteome Research
- Publisher
- American Chemical Society (ACS)
- Published
- 2010-03-12
- Volume
- 9
- Issue
- 4
- Pages
- 1754-1762
- ISSN
- 1535-3893 (ISSN-L)
- 1535-3907
- OpenAlex
- W1975822477
- Cited by
- 209
- References
- 5
- FWCI
- 4.92
- Type
- journal article
- Language
- en
- Open access
- closed
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