The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
One-Step Cloning and Chromosomal Integration of DNA
Abstract
We describe "clonetegration", a method for integrating DNA into prokaryotic chromosomes that approaches the simplicity of cloning DNA within extrachromosomal vectors. Compared to existing techniques, clonetegration drastically decreases the time and effort needed for integration of single or multiple DNA fragments. Additionally, clonetegration facilitates cloning and expression of genetic elements that are impossible to propagate within typical multicopy plasmids.
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Cites
Probing Gene Expression in Live Cells, One Protein Molecule at a Time
Yu, J., Xiao, J., Ren, X., Lao, K., & Xie, X. S. (2006) · Science · doi:10.1126/science.1119623 · Cited by 918
Cited by
Gupta, A., Reizman, I. M. B., Reisch, C. R., & Prather, K. L. J. (2017) · Nature Biotechnology · doi:10.1038/nbt.3796 · Cited by 490
Jiang, W., Zhao, X., Gabrieli, T., Lou, C., Ebenstein, Y., & Zhu, T. F. (2015) · Nature Communications · doi:10.1038/ncomms9101 · Cited by 267
Hfq CLASH uncovers sRNA-target interaction networks linked to nutrient availability adaptation
Iosub, I. A., van Nues, R. W., McKellar, S. W., Nieken, K. J., Marchioretto, M., Sy, B., Tree, J. J., Viero, G., & Granneman, S. (2020) · eLife · doi:10.7554/elife.54655 · Cited by 98
Gene silencing with CRISPRi in bacteria and optimization of dCas9 expression levels
Depardieu, F., & Bikard, D. (2020) · Methods · doi:10.1016/j.ymeth.2019.07.024 · Cited by 86
Robust direct digital-to-biological data storage in living cells.
Yim, S. S., McBee, R. M., Song, A. M., Huang, Y., Sheth, R. U., & Wang, H. H. (2021) · Nature Chemical Biology · doi:10.1038/s41589-020-00711-4 · Cited by 83
Metadata
- Authors
- St-Pierre, F., Cui, L., Priest, D. G., Endy, D., Dodd, I. B., & Shearwin, K. E.
- Venue
- ACS Synthetic Biology
- Publisher
- American Chemical Society (ACS)
- Published
- 2013-05-20
- Volume
- 2
- Issue
- 9
- Pages
- 537-541
- ISSN
- 2161-5063 (ISSN-L)
- OpenAlex
- W2325480448
- Cited by
- 216
- References
- 1
- FWCI
- 8.09
- Type
- journal article
- Language
- en
- Open access
- closed
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- CoChat. (2026). Citation check: One-Step Cloning and Chromosomal Integration of DNA. https://app.cochat.ai/papers/10.1021/sb400021j
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- St-Pierre, F., Cui, L., Priest, D. G., Endy, D., Dodd, I. B., & Shearwin, K. E. (2013). One-Step Cloning and Chromosomal Integration of DNA. ACS Synthetic Biology. https://doi.org/10.1021/sb400021j
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
