Maillard reaction products modulating the growth of human tumor cells in vitro
Abstract
We investigated the effect of a series of Maillard reaction products formed from carbohydrates under household heating conditions on the growth of human tumor cells in vitro. 4-Hydroxy-5-methyl-3-(2H)-furanone (1) was found to potently enhance the proliferation of human tumor cells. In contrast, the Maillard-type chromophores 2-(2-furyl)methylidene-4-hydroxy-5-methyl-2H-furan-3-one (2), 4-(2-furyl)-7-[(2-furyl)methylidene]-2-hydroxy-2H,7H,8aH-pyrano[2,3-b]- pyran-3-one (6), and 3-hydroxy-4[(E)-(2-furyl)methylidene]methyl-3-cyclopentene-1,2 dione (13) inhibited the growth of human tumor cells in vitro in the low micromolar range. GXF251L cells (gastric carcinoma), synchronized by serum deprivation, were retained in the G1-phase of the cell cycle after treatment with 2, 6, or 13 for 24 h. Concomitantly, a distinct sub-G1 peak was observed, indicative for apoptosis induction. DNA fragmentation was further investigated by ELISA using antibodies raised against histones and DNA. 2 induced a significant increase of fragmented DNA at concentrations > or = 30 microM. After treatment with compound 6, DNA fragmentation was observed at a higher concentration range (> or = 50 microM), whereas incubation with 13 resulted in a marked DNA fragmentation already at 20 microM. On the protein level, the activation of caspase 3, as an early marker for apoptosis induction, was determined. The results were almost identical to those obtained in the DNA fragmentation ELISA. In summary, Maillard reaction products potently modulating the growth of human tumor cells were identified. The Maillard-type chromophores 2, 6, and 13 were found to interfere with the proliferation of gastric carcinoma cells, causing cell cycle arrest and apoptosis induction.
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- Authors
- Marko, D., Habermeyer, M., Kemény, M., Weyand, U., Niederberger, E., Frank, O., & Hofmann, T.
- Affiliations
- University of Kaiserslautern (DE)
- Corresponding author
- Marko, Doris
- Venue
- Chemical Research in Toxicology
- Publisher
- American Chemical Society (ACS)
- Published
- 2002-12-07
- Volume
- 16
- Issue
- 1
- Pages
- 48-55
- ISSN
- 0893-228X (ISSN-L)
- 1520-5010
- OpenAlex
- W1976367795
- Cited by
- 58
- References
- 11
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 3 · 2023: 2 · 2022: 4 · 2021: 2 · 2020: 3 · 2019: 1 · 2018: 2 · 2017: 3 · 2016: 2 · 2015: 5 · 2014: 2 · 2013: 2 · 2012: 3
- FWCI
- 0.65
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Chemical Research in Toxicology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Clinical Biochemistry › Advanced Glycation End Products research
- Life Sciences › Agricultural and Biological Sciences › Food Science › Potato Plant Research
- Physical Sciences › Chemistry › Organic Chemistry › Synthesis and biological activity
- Keywords
- Maillard reaction products, gastric cancer cells, apoptosis induction, cell cycle arrest, G1 cell cycle arrest, DNA fragmentation, caspase-3 activation, furanone
- MeSH terms
- Antineoplastic Agents, Cell Cycle, Cyclopentanes, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Formazans, Furans, Humans, Neoplasms, Tetrazolium Salts, Tumor Cells, Cultured, Maillard Reaction, Apoptosis, Caspases, Caspase 3, DNA Fragmentation
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