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Cited by 11Animal Genetics and ReproductionViral Infectious Diseases and Gene Expression in InsectsVirus-based gene therapy research

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

A One-Step Gene Amplification System for Use in Cultured Mammalian Cells and Transgenic Animals

Melton, David W., Ketchen, Ann-Marie, Kind, Alexander J., McEwan, Carolanne, Paisley, Derek, Selfridge, Jim·2001·Transgenic Research
DOI 10.1023/a:1008951732020
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Abstract

Gene amplification is widely used for the production of pharmaceuticals and therapeutics in situations where a mammalian system is essential to synthesise a fully active product. Current gene amplification systems require multiple rounds of selection, often with high concentrations of toxic chemicals, to achieve the highest levels of gene amplification. The use of these systems has not been demonstrated in specialised mammalian cells, such as embryonic-stem cells, which can be used to generate transgenic animals. Thus, it has not yet proved possible to produce transgenic animals containing amplified copies of a gene of interest, with the potential to synthesise large amounts of a valuable gene product. We have developed a new amplification system, based around vectors encoding a partially disabled hypoxanthine phosphoribosyltransferase (HPRT) minigene, which can achieve greater than 1000-fold amplification of HPRT and the human growth hormone gene in a single step in Chinese hamster-lung cells. The amplification system also works in mouse embryonic-stem cells and we have used it to produce mice which express 30-fold higher levels of human protein C in milk than obtained with conventional transgenesis using the same protein C construct. This system should also be applicable to large animal transgenics produced by nuclear transfer from cultured cell lines.

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Crossref · 10.1023/a:1008951732020

Cites

1 of 1 references verified0 citation errors · 0 not checkable

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  • Other selection systems developed more recently include the OSCAR system, which uses minigene vectors encoding hypoxanthine phosphoribosyltransferase (HPRT), essential for purine synthesis.

    “Other selection systems have been developed more recently, such as the OSCAR™ system from the University of Edinburgh, that uses minigene vectors encoding the hypoxanthine phosphoribosyltransferase (HPRT), essential for purine synthesis (Melton et al., 2001;Costa et al., 2012).”

    From Therapeutic glycoprotein production in mammalian cells (2017) · cites this paper as Melton et al., 2001

Citations

Cites

  • Repeat-induced gene silencing in mammals

    Garrick, D., Fiering, S., Martin, D. I. K., & Whitelaw, E. (1998) · Nature Genetics · doi:10.1038/ng0198-56 · Cited by 887 · cited 1×

    “Repeat-induced gene silencing has been reported for conventional transgene tandem arrays ( Garrick et al., 1998 ).”
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Flagged first, then by citations

Research works printed but not linked: 24

  1. Campbell KHS, McWhir J, Ritchie WA and Wilmut I (1996) Sheep cloned by nuclear transfer from a cultured cell line. Nature 380: 64–66. · DOI
  2. Carver AS, Dalrymple MA, Wright G, Cottom DS, Reeves DB, Gibson YH, Keenan JL, Barrass JD, Scott AR, Colman A and Garner I (1993) Transgenic livestock as bioreactors: stable expression of human alpha-1 antitrypsin by a flock of sheep. Bio/Technology 11: 1263–1270.
  3. Fort P, Marty L, Piechaczyk M, el Sabrouty S, Dani C, Jeanteur P and Blanchard JM (1985) Various rat adult tissues express only one major mRNA species from the glyceraldehyde-3-phosphate dehydrogenase multigene family. Nucl Acids Res 13: 1431–1442. · DOI
  4. Fuscoe JC, Fenwick RG Jr, Ledbetter DH and Caskey CT (1983) Deletion and amplification of the HPRT locus in Chinese hamster cells. Mol Cell Biol 3: 1086–1096.
  5. Gordon JW and Isola LM (1993) Spontaneous amplification of a foreign dihydrofolate reductase gene in transgenic mice. Transgene 1: 77–90.
  6. Kellems RE (1993) Gene Amplification in Mammalian Cells. Marcel Dekker, New York, USA.
  7. Konecki DS, Brennand J, Fuscoe JC, Caskey CT and Chinault AC (1982) Hypoxanthine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. Nucl Acids Res 10: 6763–6775. · DOI
  8. Kozak M (1986) Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes. Cell 44: 283–292. · DOI · Paper page
  9. Littlefield JW (1964) Selection of hybrids from matings of fibroblasts in vitro and their presumed recombinants. Science 145: 709. · DOI
  10. Livingstone LR, White A, Sprouse J, Livanos E, Jacks T and Tlsty TD (1992) Altered cell cycle arrest and gene amplification potential accompany loss of wild-type p53. Cell 70: 923–935. · DOI
  11. Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurements with the folin phenol reagents. J Biol Chem 193: 265–275. · DOI
  12. Magin TM, McEwan C, Milne M, Pow AM, Selfridge J and Melton DW (1992a) A position and orientation dependent element in the first intron is required for expression of the mouse HPRT gene in embryonic stem cells. Gene 122: 289–296. · DOI
  13. Magin TM, McWhir J and Melton DW (1992b) A new mouse embryonic stem cell line with good germline contribution and gene targeting frequency. Nucl Acids Res 20: 3795–3796. · DOI
  14. McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE and Kind AJ (2000) Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature 405: 1066–1069. · DOI
  15. Melton DW, Brennand J, Ledbetter DH, Konecki DS, Chinault AC and Caskey CT (1982) Phenotypic reversion at the HPRT locus as a consequence of gene amplication. In: Schimke RT (ed.), 142 Gene Amplification (pp 59–65) Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
  16. Melton DW, McEwan C, McKie A and Reid AM (1986). Expression of the mouse HPRT gene: Deletional analysis of the promoter region of an X-chromosome linked housekeeping gene. Cell 44: 319–328. · DOI
  17. Moore RC, Redhead NJ, Selfridge J, Hope J, Manson JC and Melton DW (1995) Double replacement gene targeting for the production of a series of mouse strains with different prion protein gene alterations. Bio/Technology 13: 999–1004.
  18. Paleyanda RK, Zhang DW, Hennighausen L, McKnight RA and Lubon H (1994) Regulation of human protein C gene expression by the mouse WAP promoter. Transgenic Res 3: 335–343. · DOI
  19. Paulson TG, Almasan A, Brody LL and Wahl GM (1998) Gene amplification in a p53-deficient cell line requires cell cycle progression under conditions that generate DNA breakage. Mol Cell Biol 18: 3089–3100. · DOI
  20. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M et al. (1997) Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science 278: 2130–2133. · DOI
  21. Thompson S, Clarke AR, Pow AM, Hooper ML and Melton DW (1989) Germ line transmission of a corrected HPRT gene produced by gene targeting in embryonic stem cells. Cell 56: 313–321. · DOI
  22. Tuddenham EGD and Cooper DN (1994) In: The Molecular Genetics of Haemostasis and its inherited Disorders (pp 149–163) Oxford University Press, Oxford.
  23. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KHS (1997) Viable offspring derived from foetal and adult mammalian cells. Nature 385: 810–813. · DOI
  24. Wu CL and Melton DW (1993) Production of a model for Lesch-Nyhan syndrome in hypoxanthine phosphoribosyltransferasedeficient mice. Nature Genet 3: 235–240. · DOI · Paper page

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Metadata

Authors
Melton, D. W., Ketchen, A.-M., Kind, A. J., McEwan, C., Paisley, D., & Selfridge, J.
Affiliations
  • Western General Hospital (GB)
  • University of Edinburgh (GB)
Corresponding author
Melton, David W.
ORCID
Selfridge, Jim
Venue
Transgenic Research
Publisher
Springer Science and Business Media LLC
Published
2001-04
Volume
10
Issue
2
Pages
133-142
ISSN
  • 0962-8819 (ISSN-L)
  • 1573-9368
DOI
10.1023/a:1008951732020
OpenAlex
W77897694
Cited by
11
References
1
Citations by year
2024: 1 · 2019: 1 · 2017: 1 · 2014: 1 · 2013: 2 · 2012: 3
FWCI
0.00
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Transgenic Research (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Virus-based gene therapy research
Keywords
gene amplification, HPRT, human growth hormone, protein C, transgenic mice, mouse embryonic stem cells, Chinese hamster lung cells, transgenic animals, recombinant protein expression, nuclear transfer
MeSH terms
Animals, Cells, Cultured, Cricetulus, Embryo, Mammalian, Gene Amplification, Guinea Pigs, Cricetinae, Humans, Hypoxanthine Phosphoribosyltransferase, Milk, Protein C, RNA, Messenger, Stem Cells, Animals, Genetically Modified

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