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Overexpression of Plant Uncoupling Mitochondrial Protein in Transgenic Tobacco Increases Tolerance to Oxidative Stress
Abstract
An Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northern blot, revealed variable levels of transgene expression. Antibody probing of Western blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significant increase in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide the first biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.
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UCPs are able to induce mild uncoupling, increasing the rate of respiration and decreasing the production of reactive oxygen species.
“They are able to induce mild uncoupling, thus increasing the rate of respiration and decreasing the production of reactive oxygen species (ROS) (24-27).”
From Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans (2004) · cites this paper as [24]
UCPs are involved in cell defense against oxidative stress.
“Evidence has been presented that the new UCPs are involved in cell defense against oxidative stress (22-27).”
From Respiration, oxidative phosphorylation, and uncoupling protein in Candida albicans (2004) · cites this paper as [24]
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Cardozo, L. D. M., de Almeida, F. A. N., Fioresi, V. S., Canal, G. B., Mengarda, L. H. G., Ferreira, A., & Ferreira, M. F. D. S. (2025) · Scientific Reports · doi:10.1038/s41598-025-01436-x · Cited by 1
Nunes-Laitz, A. V. (2014) · Americanae (AECID Library)
Fávaro, R. D. (2008) · Americanae (AECID Library)
Participação da mitocôndria na regulação da viabilidade celular.
Kowaltowski, A. J. (2007) · Universidade de São Paulo · doi:10.11606/t.46.2007.tde-08102007-145540
Study of the photosynthetic responce to drought stress in AOX mutants of Arabidopsis thaliana
Coito, J. L. (2009) · Portuguese National Funding Agency for Science, Research and Technology (RCAAP Project by FCT)
Kim, M., Lee, U., Small, I., Francs‐Small, C. C. D., & Vierling, E. (2012) · ScholarWorks@UMassAmherst (University of Massachusetts Amherst)
Valente, C. (2012) · LA Referencia (Red Federada de Repositorios Institucionales de Publicaciones Científicas)
Protein Quality Control, Redox Balance and Heat Stress Tolerance in Arabidopsis
Kim, M. (2011) · UA Campus Repository (The University of Arizona)
Metadata
- Authors
- Brandalise, M., Maia, I. G., Borecký, J., Vercesi, A. E., & Arruda, P.
- Affiliations
- Universidade Estadual de Campinas (UNICAMP) (BR)
- Universidade Estadual Paulista (Unesp) (BR)
- Corresponding author
- Arruda, Paulo
- Venue
- Journal of Bioenergetics and Biomembranes
- Published
- 2003-06
- Volume
- 35
- Issue
- 3
- Pages
- 203-209
- ISSN
- 0145-479X (ISSN-L)
- 1573-6881
- OpenAlex
- W1599293621
- Cited by
- 76
- References
- 39
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 1 · 2020: 2 · 2019: 2 · 2017: 3 · 2016: 1 · 2015: 2 · 2014: 5 · 2013: 2 · 2012: 6
- FWCI
- 9.04
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Bioenergetics and Biomembranes (publishedVersion)
- PubMed (publishedVersion)
- Acervo Digital da Universidade Estadual Paulista (Universidade Estadual Paulista) (submittedVersion)
- UNESP Institutional Repository (São Paulo State University) (submittedVersion)
- Funders
- Fundação de Amparo à Pesquisa do Estado de São Paulo
- Conselho Nacional de Desenvolvimento Científico e Tecnológico
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Mitochondrial Function and Pathology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Keywords
- uncoupling proteins, mitochondrial uncoupling, oxidative stress resistance, hydrogen peroxide, transgenic tobacco, Arabidopsis thaliana, plant stress protection
- MeSH terms
- Uncoupling Protein 1, Carrier Proteins, Hydrogen Peroxide, Ion Channels, Membrane Proteins, Plant Proteins, RNA, Messenger, Nicotiana, Arabidopsis, Oxidative Stress, Protective Agents, Mitochondrial Proteins, Plants, Genetically Modified
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