A nude mouse model of human osteosarcoma lung metastases for evaluating new therapeutic strategies
Abstract
The purpose of these studies was to develop a metastatic osteosarcoma nude mouse model to evaluate the in vivo efficacy of new therapeutic compounds. Human SAOS-2 osteosarcoma cells (10(6) cells) were injected i.v. into nude mice. Cells isolated from a rare pulmonary metastases 6 months later were established (SAOS-LM1) in culture and re-injected. This procedure was repeated 5 additional times to produce the SAOS-LM6 cell line. Visible pulmonary nodules were present 8 weeks following i.v. injection of 10(6) SAOS-LM6 cells as compared to 17 weeks using SAOS-LM2 cells. Microscopic SAOS-LM6 pulmonary metastases were demonstrated at 6 weeks. Administration of adriamycin on week 9 resulted in regression of macroscopic SAOS-LM6 lung tumors. The ability of the model to be used to evaluate the effectiveness of a biologic agent against microscopic disease was also verified. It was concluded that this model can assess therapeutic efficacy and therefore, may have a role in investigating the potential of novel approaches aimed at eliminating pulmonary metastatic osteosarcoma.
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Companion cell lines with enhanced lung colonization capacity have enabled study of metastasis-driving mechanisms.
“Companion cell lines with enhanced lung colonization capacity, such as SaOS2-LM7, MG63.3 and 143B 209,210,211 , have enabled the study of metastasis-driving mechanisms 50,212 .”
From Osteosarcoma (2022) · cites this paper as [209]
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- Authors
- Jia, S.-F., Worth, L. L., & Kleinerman, E. S.
- Affiliations
- The University of Texas MD Anderson Cancer Center (US)
- Venue
- Clinical & Experimental Metastasis
- Published
- 1999-10
- Volume
- 17
- Issue
- 6
- Pages
- 501-506
- ISSN
- 0262-0898 (ISSN-L)
- 1573-7276
- OpenAlex
- W186831022
- Cited by
- 146
- References
- 20
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4 · 2023: 3 · 2022: 5 · 2021: 4 · 2020: 10 · 2019: 5 · 2018: 7 · 2017: 5 · 2016: 6 · 2015: 4 · 2014: 4 · 2013: 5 · 2012: 8
- FWCI
- 2.88
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Clinical & Experimental Metastasis (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Cancer Institute (CA-42992, CA-16672, R01CA042992, P30CA016672)
- Topics
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Sarcoma Diagnosis and Treatment
- Health Sciences › Medicine › Oncology › Cancer Cells and Metastasis
- Health Sciences › Medicine › Oncology › Bone health and treatments
- Keywords
- osteosarcoma, lung metastasis, nude mouse model, Saos-2 cells, doxorubicin, treatment efficacy assessment, biologic agents
- MeSH terms
- Animals, Antineoplastic Agents, Disease Models, Animal, Doxorubicin, Drug Screening Assays, Antitumor, Etoposide, Humans, Lung Neoplasms, Mice, Nude, Neoplasm Transplantation, Osteosarcoma, Vinblastine, Cytokines, Mice
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