Cited by 48Insect Resistance and GeneticsInsect-Plant Interactions and ControlGenetically Modified Organisms Research

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The −689/+197 region of the maize protease inhibitor gene directs high level, wound inducible expression of the cry1B gene which protects transgenic rice plants from stemborer attack

Breitler, Jean Christophe, Cordero, Maria José, Royer, Monique, Meynard, Donaldo, San Segundo, Blanca, Guiderdoni, Emmanuel·2001·Molecular Breeding

DOI 10.1023/a:1011609128319

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Abstract

To investigate the activity of the regulatory region of the maize (Zea mays L.) proteinase inhibitor (mpi) gene, we transferred into rice (Oryza sativa L.) plants the -689/+197 (C1) fragment of the mpi genomic clone fused to either the uidA gene or a synthetic Bacillus thuringiensis cry1B gene. Although uidA and cry1B encode very different proteins consistent results were obtained from their respective histochemical and fluorometric and immunoblot detections in T3 transgenic rice lines. In response to mechanical wounding, a 4-5 fold increase in GUS activity and a Cry1B accumulation reaching 0.1-0.2% of total soluble proteins were observed from basal and undetectable levels respectively in leaf tissue. The establishment of the time-course of wound response in both systems revealed a maximum induction level 12-16 h after treatment. From both systems we also deduced that the C1 region is not active in pollen and seed endosperm. Three independent transformation events expressing cry1B under the control of the C1 region exhibited protection against striped stem borer damage and showed 100% mortality of second instar larvae 8 days after release. These results illustrate the first evidence that wound-inducible expression of a Bacillus thuringiensis endotoxin gene affords full protection to transgenic rice plants.

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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.
Jefferson, R., Kavanagh, T. A., & Bevan, M. W. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02730.x · Cited by 9,854
Assaying chimeric genes in plants: The GUS gene fusion system
Jefferson, R. (1987) · Plant Molecular Biology Reporter · doi:10.1007/bf02667740 · Cited by 4,455
Protease Inhibitors In Plants: Genes For Improving Defenses Against Insects And Pathogens
CA, R. (1990) · Annual Review of Phytopathology · doi:10.1146/annurev.py.28.090190.002233 · Cited by 1,593
Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
Odell, J. T., Nagy, F., & Chua, N. (1985) · Nature · doi:10.1038/313810a0 · Cited by 1,419
Interplant communication: airborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves.
Farmer, E. E., & Ryan, C. A. (1990) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.87.19.7713 · Cited by 1,362
Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants
Christensen, A. H., & Quail, P. H. (1996) · Transgenic Research · doi:10.1007/bf01969712 · Cited by 1,175
A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins
Pearce, G., Strydom, D. J., Johnson, S., & Ryan, C. A. (1991) · Science · doi:10.1126/science.253.5022.895 · Cited by 1,079
DEFENSE-RELATED PROTEINS IN HIGHER PLANTS
Bowles, D. J. (1990) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.59.070190.004301 · Cited by 1,043

Show all 46 references

Research works printed but not linked: 3

Chaïr H., Legavre T. and Guiderdoni E. 1996. Some factors influencing production of transgenic plants in PEG-mediated transformation of rice protoplasts In: G.S. Khush (Ed.) Rice Genetics III, IRRI, Manila, Philippines, pp. 703–709.
Hoisington D. 1992. Laboratory Protocols. CIMMYT Applied Molecular Genetics Laboratory, Mexico DF.
Wünn J., Klöti A., Burkhardt P.K., Ghosh Biswas G.C., Launis K., Iglesias V.A. and Potrykus I. 1996. Transgenic Indica rice breeding ligne IR58 expressing a synthetic cry1Ab gene from Bacilllus thuringiensis provides effective pest control. Bio/technology 14: 171–176.

Recent advances in rice biotechnology—towards genetically superior transgenic rice
Bajaj, S., & Mohanty, A. (2005) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2005.00130.x · Cited by 242
Genetically engineered crops help support conservation biological control
Romeis, J., Naranjo, S. E., Meissle, M., & Shelton, A. M. (2019) · Biological Control · doi:10.1016/j.biocontrol.2018.10.001 · Cited by 231
“The feasibility of this approach has been documented in the glasshouse and in the field for rice where cry genes were driven by the woundinducible mpi promotor from maize (Breitler et al., 2001, 2004).”
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Kathuria, H., Giri, J., Tyagi, H., & Tyagi, A. K. (2007) · Critical Reviews in Plant Sciences · doi:10.1080/07352680701252809 · Cited by 92
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Vila, L., Quilis, J., Meynard, D., Breitler, J., Marfà, V., Murillo, I., Vassal, J. M., Messeguer, J., Guiderdoni, E., & Segundo, B. S. (2005) · Plant Biotechnology Journal · doi:10.1111/j.1467-7652.2004.00117.x · Cited by 85
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Vain, P., James, V. A., Worland, B., & Snape, J. W. (2002) · Theoretical and Applied Genetics · doi:10.1007/s00122-002-1039-5 · Cited by 74
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Moreno, A. B., Peñas, G., Rufat, M., Bravo, J. M., Estopà, M., Messeguer, J., & Segundo, B. S. (2005) · Molecular Plant-Microbe Interactions · doi:10.1094/mpmi-18-0960 · Cited by 73
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Chen, H., Lin, Y., & Zhang, Q. (2009) · Chinese Science Bulletin · doi:10.1007/s11434-009-0645-x · Cited by 66
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Authors: Breitler, J. C., Cordero, M. J., Royer, M., Meynard, D., San Segundo, B., & Guiderdoni, E.
Affiliations: Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
Consejo Superior de Investigaciones Científicas (ES)
Corresponding author: Guiderdoni, Emmanuel
ORCID: Breitler, Jean Christophe
Cordero, Maria José
Royer, Monique
Meynard, Donaldo
Guiderdoni, Emmanuel
Venue: Molecular Breeding
Publisher: Springer Science and Business Media LLC
Published: 2001-12
Volume: 7
Issue: 4
Pages: 259-274
ISSN: 1380-3743 (ISSN-L)
1572-9788
DOI: 10.1023/a:1011609128319
OpenAlex: W1253697888
Cited by: 48
References: 46
Citations by year: 2026: 1 · 2025: 2 · 2024: 2 · 2022: 2 · 2021: 1 · 2020: 2 · 2019: 2 · 2018: 3 · 2016: 2 · 2014: 2 · 2013: 5 · 2012: 2
FWCI: 0.58
Type: journal article
Language: en
Open access: closed
Licence: www.springernature.com/gp/researchers/text-and-data-mining (tdm)
www.springernature.com/gp/researchers/text-and-data-mining (vor)
Locations: Molecular Breeding (publishedVersion)
Agritrop (Cirad) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
Keywords: Bacillus thuringiensis endotoxin, wound-inducible gene expression, transgenic rice, Chilo suppressalis, insect resistance, promoter activity, GUS reporter

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The −689/+197 region of the maize protease inhibitor gene directs high level, wound inducible expression of the cry1B gene which protects transgenic rice plants from stemborer attack

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