Cited by 93Insect Resistance and GeneticsGenetically Modified Organisms ResearchInsect-Plant Interactions and Control

Bt rice harbouring cry genes controlled by a constitutive or wound‐inducible promoter: protection and transgene expression under Mediterranean field conditions

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Breitler, Jean Christophe, Vassal, Jean Michel, Del Mar Catala, Maria, Meynard, Donaldo, Marfà, Victoria, Melé, Enric, Royer, Monique, Murillo, Isabel +3 more·2004·Plant Biotechnology Journal

Abstract

Seven homozygous transgenic lines of two European commercial cultivars of rice (Ariete (A) and Senia (S)), harbouring the cry1B or cry1Aa Bacillus thuringiensis (Bt) delta-endotoxin genes, were field evaluated for protection from striped stem borer (SSB) (Chilo suppressalis) damage during the 2001 and 2002 summer crop seasons in the Delta de l'Ebre region, Spain. The plant codon-optimized toxin gene was placed under the control of the promoter of either the constitutive ubi1 gene or the wound-inducible mpi gene from maize. Stable, high-level, insecticidal protein accumulation was observed throughout root, leaf and seed tissues of field-grown plants harbouring the cry1B (lines A64.1, A33.1, A3.4 and S98.9) or cry1Aa (lines S05.1 and A19.14) genes under the control of the ubi1 promoter. Conversely, no toxin was detected in unwounded vegetative tissues of the A9.1 line harbouring the cry1B gene controlled by the mpi promoter, indicating that natural environmental stresses did not trigger the activity of the wound-inducible promoter. However, the toxin accumulated at 0.2% total soluble proteins in A9.1 sheath tissue exhibiting brown lesions resulting from SSB damage. The agronomical traits and performance of the transgenic lines were generally comparable with parental controls, except in the two lines accumulating Cry1Aa, which exhibited a high frequency of plants non-true to type. Natural infestation was assisted with manual infestations of L2/L3 SSB larvae in border control plants surrounding the experimental plots, which served as a reservoir for the second-cycle SSB population. The observation of damage (brown lesions and dead hearts) during the crop season and dissection of plants at harvest stage revealed a range of protection amongst the transgenic lines, which was highly consistent with the level of toxin accumulation and with previous experience in greenhouse assays. Lines A3.4 and S05.1 were found to exhibit stable and full protection against SSB attacks, mediated by the accumulation of Cry1B and Cry1Aa toxin, respectively, which was comparable with that afforded by the spraying of chemical insecticides on control plants. The wound-induced A9.1 line exhibited a satisfactory level of protection, with a notably low level of penetration of SSB larvae in the stems, but higher external symptoms than constitutive lines, probably due to the time lag to benefit from the protective effect of Cry1B.

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  • The feasibility of using wound-inducible promoters to drive cry genes has been documented in glasshouse and field for rice where cry genes were driven by the wound-inducible mpi promotor from maize.

    “The feasibility of this approach has been documented in the glasshouse and in the field for rice where cry genes were driven by the woundinducible mpi promotor from maize (Breitler et al., 2001(Breitler et al., , 2004).”

    From Genetically engineered crops help support conservation biological control (2019) · cites this paper as Breitler et al., 2004

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  1. Cheng, X., Sardana, R., Kaplan, H. and Altosaar, I. (1998) Agrobacterium-transformed rice plants expressing synthetic cry1Ab and cry1Ac genes are highly toxic to striped stemborer and yellow stemborer. Proc. Natl. Acad. Sci. USA, 95, 2672-2772.
  2. Nayak, P., Basu, D., Das, S., Basu, A., Ghosh, D., Ramakrishnan, N.A., Ghosh, M. and Sen, S.K. (1997) Transgenic elite indica rice plants expressing Cry1Ac-endotoxin of Bacillus thuringiensis are resist- ant against yellow stemborer (Scirpophaga incertulas). Proc. Natl. Acad. Sci. USA, 94, 2111-2116.

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Authors
Breitler, J. C., Vassal, J. M., Del Mar Catala, M., Meynard, D., Marfà, V., Melé, E., Royer, M., Murillo, I., San Segundo, B., Guiderdoni, E., & Messeguer, J.
Affiliations
  • Centre de Coopération Internationale en Recherche Agronomique pour le Développement (FR)
  • Institute of Agrifood Research and Technology (ES)
  • Consejo Superior de Investigaciones Científicas (ES)
Corresponding author
Guiderdoni, Emmanuel
Venue
Plant Biotechnology Journal
Publisher
Wiley
Published
2004-08-20
Volume
2
Issue
5
Pages
417-430
ISSN
  • 1467-7644 (ISSN-L)
  • 1467-7652
OpenAlex
W2112483255
Cited by
93
References
40
Citations by year
2025: 1 · 2024: 1 · 2023: 1 · 2022: 4 · 2021: 7 · 2020: 8 · 2019: 2 · 2018: 5 · 2017: 5 · 2016: 4 · 2015: 7 · 2014: 6 · 2013: 7 · 2012: 2
FWCI
1.66
Type
journal article
Language
en
Open access
Locations
  • Plant Biotechnology Journal (publishedVersion)
  • PubMed (publishedVersion)
  • Agritrop (Cirad) (submittedVersion)
Funders
European Commission
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Insect Resistance and Genetics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Genetically Modified Organisms Research
  • Life Sciences › Agricultural and Biological Sciences › Insect Science › Insect-Plant Interactions and Control
Keywords
Bt rice, Cry1B, Cry1Aa, Bacillus thuringiensis delta-endotoxin, Chilo suppressalis, transgenic rice, wound-inducible promoter, transgene expression

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