Immunogold study of interendothelial junction-associated and glucose transporter proteins during postnatal maturation of the mouse blood-brain barrier
Abstract
The distribution of glucose transporter (GLUT-1) and of interendothelial junction-associated proteins--zonula occludens protein (ZO-1), occludin, and beta-catenin--was studied using quantitative immunogold procedure. Lowicryl K4M-embedded samples of the cerebral cortex of 1-, 7-, and 14-day-, and 6-week-old (young-adult) mice were used. Ultrathin sections were exposed to specific rabbit polyclonal antibodies followed by colloidal gold-labelled secondary antibodies. We found that the density of immunosignals for GLUT-1 in both luminal and abluminal plasma membranes of the endothelial cells, and those closely related to the interendothelial junctions was low in blood microvessels from newborn mice, dropped slightly at the 7th day, and increased through the 14th day to the level of mature blood-brain barrier (BBB) observed in 6-week-old mice. The expression of ZO-1 was high in newborn mice and increased at the 7th day to the level similar to that found in 14-day- and 6-week-old mice. The expression of occludin was less intense than that of ZO-1 and increased from birth, reaching at the 14th day the level typical for mature BBB found in young-adult animals. The immunosignals for occludin were sparsely distributed inside the junctional clefts. Such a distribution indicates that the tight junctional characteristics are limited to a few short segments of the entire interendothelial cleft. The density of immunosignals for beta-catenin was lowest, and it had the tendency to a gradual,…
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Taken together, the findings point to a selective suppression in cholesterol synthesis in the developing brain starting at about the time that closure of the blood brain barrier is purported to occur in the mouse.
“Taken together then, all these findings point to a selective suppression in cholesterol synthesis in the developing brain starting at about the time that closure of the blood brain barrier is purported to occur in the mouse (Vorbrodt et al., 2001).”
From Suppression of brain cholesterol synthesis in male Mecp2-deficient mice is age dependent and not accompanied by a concurrent change in the rate of fatty acid synthesis (2017) · cites this paper as Vorbrodt et al., 2001
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- Authors
- Vorbrodt, A. W., Dobrogowska, D. H., & Tarnawski, M.
- Affiliations
- New York State Office for People With Developmental Disabilities (US)
- Corresponding author
- Dobrogowska, D.H.
- Venue
- Journal of Neurocytology
- Published
- 2001-08
- Volume
- 30
- Issue
- 8
- Pages
- 705-716
- ISSN
- 0300-4864 (ISSN-L)
- 1573-7381
- OpenAlex
- W329210630
- Cited by
- 47
- References
- 41
- Citations by year
- 2026: 1 · 2025: 2 · 2024: 1 · 2021: 1 · 2020: 4 · 2017: 2 · 2016: 2 · 2015: 6 · 2014: 4 · 2012: 1
- FWCI
- 1.39
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Journal of Neurocytology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Ion Transport and Channel Regulation
- Keywords
- postnatal development, GLUT1, glucose transporters, tight junction proteins, zonula occludens-1, occludin, β-catenin, endothelial junctions, cerebral microvasculature, endothelial cells, neonatal mice, cerebral cortex
- MeSH terms
- Animals, Animals, Newborn, Blood-Brain Barrier, Cell Membrane, Cerebral Arteries, Cerebral Cortex, Cytoplasm, Cytoskeletal Proteins, Endothelium, Vascular, Female, Immunohistochemistry, Male, Membrane Proteins, Mice, Inbred BALB C, Microscopy, Electron, Monosaccharide Transport Proteins, Phosphoproteins, Trans-Activators, Tight Junctions, beta Catenin, Glucose Transporter Type 1, Mice, Occludin, Zonula Occludens-1 Protein
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