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Chlorophyll Fluorescence Parameters: The Definitions, Photosynthetic Meaning, and Mutual Relationships

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Roháček, K.·2002·Photosynthetica

DOI 10.1023/a:1020125719386

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Abstract

Chlorophyll fluorescence parameters (Chl FPs) derived from the slow (long-term) induction kinetics of modulated Chl a fluorescence are reviewed and analysed with respect to their application in photosynthesis research. Only four mutually independent Chl FPs, calculated from values of five essential Chl fluorescence (ChlF) yields, are distinguished as the basic ones. These are: the maximum quantum yield of PS2 photochemistry (ΦPO), the photochemical quenching of variable ChlF (qP), the non-photochemical quenching of variable ChlF (qN), and the relative change of minimum ChlF (qO). ΦPO refers to the dark-adapted state of a thylakoid membrane, qP, qN and qO characterise the light-adapted state. It is demonstrated that all other Chl FPs can be determined using this quartet of parameters. Moreover, three FPs related to the non-radiative energy dissipation within thylakoid membranes are evaluated, namely: the non-photochemical ChlF quenching (NPQ), the complete non-photochemical quenching of ChlF (qCN), and the effective quantum yield of non-photochemical processes in PS2 (ΦN). New FPs, the total quenching of variable ChlF (qTV) and the absolute quenching of ChlF (qA) which allow to quantify co-action of the photochemical and non-photochemical processes during a light period are defined and analysed. The interpretation of Chl FPs and recommendations for their application in the photosynthesis research are also given. Some alternative FPs used in the laboratory practice have only an approximate character and can lead to incorrect conclusions if applied to stressed plants. They are reviewed and compared with the standard ones. All formulae and conclusions discussed herein are verified using experimental values obtained on young seedlings of the Norway spruce (Picea abies [L.] Karst.).

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Authors: Roháček, K.
Affiliations: Czech Academy of Sciences (CZ)
Corresponding author: Roháček, K.
ORCID: Roháček, K.
Venue: Photosynthetica
Publisher: Institute of Experimental Botany
Published: 2002-03-01
Volume: 40
Issue: 1
Pages: 13-29
ISSN: 0300-3604 (ISSN-L)
1573-9058
DOI: 10.1023/a:1020125719386
OpenAlex: W1571556317
Cited by: 724
References: 67
Citations by year: 2026: 27 · 2025: 35 · 2024: 31 · 2023: 35 · 2022: 33 · 2021: 35 · 2020: 39 · 2019: 28 · 2018: 36 · 2017: 40 · 2016: 31 · 2015: 36 · 2014: 51 · 2013: 36 · 2012: 33
FWCI: 4.16
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: Photosynthetica (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Photochemistry and Electron Transfer Studies
Physical Sciences › Environmental Science › Global and Planetary Change › Plant Water Relations and Carbon Dynamics
Keywords: photosystem II, maximum quantum yield, photochemical quenching, non-photochemical quenching, non-radiative energy dissipation, thylakoid membranes, light adaptation, seedlings, Norway spruce

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Chlorophyll Fluorescence Parameters: The Definitions, Photosynthetic Meaning, and Mutual Relationships

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