Chlorophyll fluorescence—a practical guide
Abstract
Chlorophyll fluorescence analysis has become one of the most powerful and widely used techniques available to plant physiologists and ecophysiologists. This review aims to provide an introduction for the novice into the methodology and applications of chlorophyll fluorescence. After a brief introduction into the theoretical background of the technique, the methodology and some of the technical pitfalls that can be encountered are explained. A selection of examples is then used to illustrate the types of information that fluorescence can provide.
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Fv/Fm is a good indicator of plant wellbeing, with values around 0.83 indicating a good status, and lower values revealing unfavourable conditions for the photosynthetic apparatus linked to stressful events.
“Fv/Fm is a good indicator of plant wellbeing, with values around 0.83 indicating a good status (Björkman and Demmig 1987), and lower values revealing unfavourable conditions for photosynthetic apparatus linked to stressful events (Baker and Rosenqvist 2004;Maxwell and Johnson 2000).”
From Green compost amendment improves potato plant performance on Mars regolith simulant as substrate for cultivation in space (2023) · cites this paper as Maxwell and Johnson 2000
Thermal dissipation process represents the main safety valve, avoiding photoinhibition and permanent damages to photosystems.
“Thermal dissipation process represents the main”
From Green compost amendment improves potato plant performance on Mars regolith simulant as substrate for cultivation in space (2023) · cites this paper as Maxwell and Johnson 2000
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- Authors
- Maxwell, K., & Johnson, G. N.
- Affiliations
- Newcastle University (GB)
- University of Manchester (GB)
- ORCID
- Johnson, Giles N.
- Venue
- Journal of Experimental Botany
- Publisher
- Oxford University Press (OUP)
- Published
- 2000-04
- Volume
- 51
- Issue
- 345
- Pages
- 659-668
- ISSN
- 0022-0957 (ISSN-L)
- 1460-2431
- OpenAlex
- W2132527497
- Cited by
- 9,347
- References
- 1
- Citations by year
- 2026: 542 · 2025: 515 · 2024: 545 · 2023: 516 · 2022: 561 · 2021: 651 · 2020: 568 · 2019: 542 · 2018: 526 · 2017: 503 · 2016: 471 · 2015: 442 · 2014: 506 · 2013: 381 · 2012: 356
- FWCI
- 13.48
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Experimental Botany (publishedVersion)
- PubMed (publishedVersion)
- Research Explorer (The University of Manchester) (submittedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Stress Responses and Tolerance
- Physical Sciences › Environmental Science › Global and Planetary Change › Plant Water Relations and Carbon Dynamics
- Keywords
- chlorophyll fluorescence, technical pitfalls, plant physiology, ecophysiology, photosynthesis
- MeSH terms
- Chlorophyll, Electron Transport, Fluorescence, Photosynthesis, Plant Physiological Phenomena
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