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Cited by 82Proteoglycans and glycosaminoglycans researchBlood Coagulation and Thrombosis MechanismsPlatelet Disorders and Treatments

The DOI matches its Crossref record; 54 of the 54 references it cites were found in a registry or an index.

Mice deficient in heparan sulfate 3-O-sulfotransferase-1: Normal hemostasis with unexpected perinatal phenotypes

Shworak, Nicholas W., HajMohammadi, Sassan, de Agostini, Ariane I., Rosenberg, Robert D.·2002·Glycoconjugate Journal
DOI 10.1023/a:1025377206600
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Abstract

Heparan sulfate that contains antithrombin binding sites is designated as anticoagulant heparan sulfate (HS(act)) since, in vitro, it dramatically enhances the neutralization of coagulation proteases by antithrombin. Endothelial cell production of HS(act) is controlled by the Hs3st1 gene, which encodes the rate limiting enzyme-heparan sulfate 3-O-sulfotransferase-1 (Hs3st1). It has long been proposed that levels of endothelial HS(act) may tightly regulate hemostatic tone. This potential in vivo role of HS(act) was assessed by generating Hs3st1(-/-) knockout mice. Hs3st1(-/-) and Hs3st1(+/+) mice were evaluated with a variety of methods, capable of detecting altered hemostatic tone. However, both genotypes were indistinguishable. Instead, Hs3st1(-/-) mice exhibited lethality on a specific genetic background and also showed intrauterine growth retardation. Neither phenotypes result from a gross coagulopathy. So although this enzyme produces the majority of tissue HS(act), Hs3st1(-/-) mice do not show an obvious procoagulant phenotype. These results suggest that the bulk of HS(act) is not essential for normal hemostasis and that hemostatic tone is not tightly regulated by total levels of HS(act). Moreover, the unanticipated non-thrombotic phenotypes suggest structure(s) derived from this enzyme might serve additional/alternative biologic roles.

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Crossref · 10.1023/a:1025377206600

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Citations

Cites

Research works printed but not linked: 2

  1. Marcum JA, Rosenberg RD, The biochemistry, cell biology, and pathophysiology of anticoagulantly active heparin-like molecules of the vessel wall. In Heparin, Chemical and Biological Properties: Clinical Applications, edited by Lane DA, Lindahl U (London, Edward Arnold, 1999), pp. 275–94.
  2. de Agostini AI, Watkins SC, Slater HS, Youssoufian H, Rosenberg RD, Localization of anticoagulantly active heparan sulfate proteoglycans in vascular endothelium: Antithrombin binding on cultured endothelial cells and perfused rat aorta, Journal of Cell Biology 111, 1293–304 (1990). · DOI

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Metadata

Authors
Shworak, N. W., HajMohammadi, S., de Agostini, A. I., & Rosenberg, R. D.
Affiliations
  • Dartmouth College (US)
  • Massachusetts Institute of Technology (US)
Corresponding author
Shworak, Nicholas W.
ORCID
Venue
Glycoconjugate Journal
Publisher
Springer Science and Business Media LLC
Published
2002-05
Volume
19
Issue
4-5
Pages
355-361
ISSN
  • 0282-0080 (ISSN-L)
  • 1573-4986
DOI
10.1023/a:1025377206600
OpenAlex
W2082080310
Cited by
77
References
52
Citations by year
2026: 1 · 2025: 1 · 2024: 3 · 2022: 2 · 2021: 1 · 2020: 1 · 2019: 2 · 2017: 2 · 2016: 4 · 2015: 3 · 2014: 5 · 2013: 5 · 2012: 2
FWCI
1.58
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Glycoconjugate Journal (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Proteoglycans and glycosaminoglycans research
  • Health Sciences › Medicine › Hematology › Blood Coagulation and Thrombosis Mechanisms
  • Health Sciences › Medicine › Hematology › Platelet Disorders and Treatments
Keywords
hemostasis, knockout mice, perinatal lethality, fetal growth restriction, coagulation, genetic background
MeSH terms
Animals, Animals, Newborn, Anticoagulants, Binding Sites, Blood Coagulation, Carbohydrate Sequence, Hemostasis, Heparan Sulfate, Molecular Sequence Data, Phenotype, Sulfotransferases, Mice, Knockout, Mice

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.