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Cited by 86Advanced Drug Delivery SystemsPhase Equilibria and ThermodynamicsInhalation and Respiratory Drug Delivery

The DOI matches its Crossref record; 39 of the 40 references it cites were found in a registry or an index.

Preparation of Large Porous Deslorelin-PLGA Microparticles with Reduced Residual Solvent and Cellular Uptake Using a Supercritical Carbon Dioxide Process

Koushik, Kavitha, Kompella, Uday B.·2004·Pharmaceutical Research
DOI 10.1023/b:pham.0000019308.25479.a4
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Abstract

Purpose The purpose of this study was to prepare large-porous peptide-encapsulating polymeric particles with low residual solvent that retain deslorelin integrity, sustain drug release, and exhibit reduced epithelial and macrophage uptake. We hypothesized that supercritical carbon dioxide (SC CO2) pressure-quench treatment of microparticles prepared using conventional approach expands these particles and extracts the residual organic solvent. Methods Initial studies with crystalline L-lactide (L-PLA) and amorphous copolymers of lactide-co-glycolide (PLGA) 50:50, 65:35, and 75:25 indicated that PLGA 50:50 was the most amenable to morphological changes upon SC CO2 treatment. Therefore, we prepared deslorelin-PLGA (50:50) microparticles using the conventional emulsion-solvent evaporation method, and in a second step equilibrated with SC CO2 at various temperatures (33-37 degrees C) and pressures (1200-2000 psi) for discrete intervals followed by rapid isothermal depressurization. The particles were then characterized for morphology, polymer thermal properties, particle size, porosity, bulk density, and residual solvent content. Also, deslorelin integrity, conformation, release, and cellular uptake before and after SC CO2 treatment was determined. Results Upon SC CO2 treatment (1200 psi, 33 degrees C for 30 min), the mean particle size of the deslorelin PLGA microparticles increased from 2.2 to 13.8 microm, the mean porosity increased from 39 to 92.38% the mean pore diameter incr…

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Crossref · 10.1023/b:pham.0000019308.25479.a4

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39 of 39 references verified0 citation errors · 0 not checkable

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  • Using supercritical CO2 as a foaming agent, organic solvents can be minimized or eliminated in the production of PLGA foams.

    “Using supercritical CO2 as a foaming agent, organic solvents can be minimized or eliminated in production of PLGA foams [66].”

    From Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier (2011) · cites this paper as [66]

  • After pressurization and rapid depressurization with CO2, thermodynamic instability leads to CO2 clustering inside the liquid polymer, and as CO2 leaves, the emulsion results in a porous polymer structure.

    “After such pressurization and rapid depressurization sequence, the thermodynamic instability of CO2 molecules leads to their clustering inside the liquid polymer. As CO2 leaves, the emulsion results in a porous polymer structure [66,67].”

    From Poly Lactic-co-Glycolic Acid (PLGA) as Biodegradable Controlled Drug Delivery Carrier (2011) · cites this paper as [66]

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1 of the 40 references couldn’t be matched to a known paper.

Research works printed but not linked: 2

  1. R. W. Woody. Studies of theoretical circular dichroism of polypeptides: Contribution of beta-turns. In E. R. Blout, F. A. Bovey, M. Goodman, and N. Lotan (eds.), Peptides, Polypeptides and Proteins, John Wiley and Sons, New York, 1974 pp. 338-360.
  2. K. Thoma and B. Schluetermann. Relationships between manufacturing parameters and pharmaceutical-technological requirements on biodegradable microparticles. Part 3: Pharmaceutical and technological investigations on the particle size distribution of local anesthetics containing biodegradable microspheres. Pharmazie 47:198-202 (1992).

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Metadata

Authors
Koushik, K., & Kompella, U. B.
Affiliations
University of Nebraska Medical Center (US)
Corresponding author
Kompella, Uday B.
ORCID
Kompella, Uday B.
Venue
Pharmaceutical Research
Publisher
Springer Science and Business Media LLC
Published
2004-03
Volume
21
Issue
3
Pages
524-535
ISSN
  • 0724-8741 (ISSN-L)
  • 1573-904X
DOI
10.1023/b:pham.0000019308.25479.a4
OpenAlex
W2058464257
Cited by
86
References
40
Citations by year
2026: 3 · 2025: 2 · 2024: 5 · 2023: 2 · 2022: 2 · 2021: 2 · 2020: 6 · 2019: 3 · 2018: 5 · 2017: 2 · 2016: 2 · 2015: 5 · 2014: 7 · 2013: 5 · 2012: 5
FWCI
3.47
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Pharmaceutical Research (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • University of Nebraska Medical Center
  • National Institutes of Health
  • National Eye Institute (R03EY013842, EY013842)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK 064172, R01DK064172)
Topics
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmaceutical Science › Advanced Drug Delivery Systems
  • Physical Sciences › Chemistry › Physical and Theoretical Chemistry › Phase Equilibria and Thermodynamics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Inhalation and Respiratory Drug Delivery
Keywords
deslorelin, PLGA microspheres, supercritical carbon dioxide, pressure quenching, porous particles, residual solvent removal, sustained drug release, peptide delivery, cellular uptake, beta-turn conformation, glass transition temperature, emulsion solvent evaporation, Calu-1 cells, macrophages, circular dichroism, drug release kinetics, bulk density
MeSH terms
Animals, Carbon Dioxide, Microscopy, Electron, Scanning, Particle Size, Polymers, Solvents, Porosity

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