The DOI matches its Crossref record; 44 of the 45 references it cites were found in a registry or an index.
Inhibition of Angiogenesis by Aβ Peptides
Abstract
Abeta peptides are naturally occurring peptides forming beta-sheet aggregates that constitute an integral component of senile plaques and vascular deposits in Alzheimer's disease. Since several peptides adopting a beta-sheet conformation have been shown to be anti-angiogenic, we investigated the effect of Abeta on angiogenesis. We show that in vitro, Abeta dose-dependently inhibits the formation of capillaries by human brain endothelial cells plated on Matrigel and stimulates capillary degeneration at high doses. Preparations of Abeta peptides containing a higher content of beta-sheet structures are more potently anti-angiogenic in vitro. Ex vivo, Abeta dose-dependently opposes angiogenesis in rat aortae and in human middle cerebral arteries. In vivo, Abeta dose dependently inhibits angiogenesis in the chick chorioallantoic membrane assay and suppresses bFGF-induced blood vessel formation in the corneal micropocket and Matrigel plug assays. Since angiogenesis is required for tumor growth, we explored the effect of Abeta on human glioblastoma (U87MG) and human lung adenocarcinoma (A549) tumors. We show that intra-tumoral injection of Abeta potently inhibits the growth and vascularization of human glioblastoma and human lung adenocarcinoma tumor xenografts in nude mice. Similarly to the intra-tumoral injection regimen, Abeta delivered intraperitoneally also suppressed the growth of human lung adenocarcinoma tumor xenografts. Altogether our data show that Abeta is an angiogenesis inhibitor.
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“In vivo, injection of Aβ into mice transplanted with human glioblastoma and lung adenocarcinoma suppresses the tumor growth.410”
From Amyloid β-based therapy for Alzheimer’s disease: Challenges, successes and future (2023) · cites this paper as [410]
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Cites
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The Blood-Brain Barrier in Health and Chronic Neurodegenerative Disorders
Zlokovic, B. V. (2008) · Neuron · doi:10.1016/j.neuron.2008.01.003 · Cited by 3,280
Amyloid β-based therapy for Alzheimer’s disease: Challenges, successes and future
Zhang, Y., Chen, H., Li, R., Sterling, K., & Song, W. (2023) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-023-01484-7 · Cited by 988
Neurovascular mechanisms of Alzheimer's neurodegeneration
Zlokovic, B. V. (2005) · Trends in Neurosciences · doi:10.1016/j.tins.2005.02.001 · Cited by 770
Review: Cerebral microvascular pathology in ageing and neurodegeneration
Brown, W. R., & Thore, C. R. (2011) · Neuropathology and Applied Neurobiology · doi:10.1111/j.1365-2990.2010.01139.x · Cited by 749
GLUT1 reductions exacerbate Alzheimer's disease vasculo-neuronal dysfunction and degeneration
Winkler, E. A., Nishida, Y., Sagare, A. P., Rege, S. V., Bell, R. D., Perlmutter, D., Sengillo, J. D., Hillman, S., Kong, P., Nelson, A. R., et al. (2015) · Nature Neuroscience · doi:10.1038/nn.3966 · Cited by 689
Metadata
- Authors
- Paris, D., Townsend, K., Quadros, A., Humphrey, J., Sun, J., Brem, S., Wotoczek-Obadia, M., DelleDonne, A., Patel, N., Obregon, D. F., Crescentini, R., Abdullah, L., Coppola, D., Rojiani, A. M., Crawford, F., Sebti, S. M., & Mullan, M.
- Affiliations
- Roskamp Institute (US)
- Moffitt Cancer Center (US)
- Corresponding author
- Mullan, Michael
- ORCID
- Venue
- Angiogenesis
- Published
- 2004-03
- Volume
- 7
- Issue
- 1
- Pages
- 75-85
- ISSN
- 0969-6970 (ISSN-L)
- 1573-7209
- OpenAlex
- W2599367511
- Cited by
- 148
- References
- 45
- Citations by year
- 2026: 3 · 2025: 7 · 2024: 9 · 2023: 8 · 2022: 6 · 2021: 5 · 2020: 6 · 2019: 6 · 2018: 5 · 2017: 2 · 2016: 6 · 2015: 8 · 2014: 6 · 2013: 7 · 2012: 7
- FWCI
- 2.60
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- Locations
- Angiogenesis (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
- Life Sciences › Neuroscience › Cellular and Molecular Neuroscience › Alzheimer's disease research and treatments
- Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
- Keywords
- amyloid-beta, angiogenesis inhibition, β-sheet conformation, Alzheimer's disease, brain endothelial cells, capillary degeneration, tumor vascularization, glioblastoma, lung adenocarcinoma, tumor xenografts, anti-angiogenic peptides
- MeSH terms
- Animals, Cells, Cultured, Endothelium, Vascular, Female, Humans, Mice, Nude, Neoplasm Transplantation, Neoplasms, Experimental, Neovascularization, Pathologic, Peptide Fragments, Transplantation, Heterologous, Amyloid beta-Peptides, Treatment Outcome, Neovascularization, Physiologic, Angiogenesis Inhibitors, Middle Cerebral Artery, Cell Line, Tumor, Cell Proliferation, Mice
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