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Cited by 119Cardiac Ischemia and ReperfusionPhytochemicals and Medicinal PlantsCardiac Fibrosis and Remodeling

Cardioprotection from ischemia and reperfusion injury by Withania somnifera: a hemodynamic, biochemical and histopathological assessment

Mohanty, Ipseeta, Gupta, Suresh Kumar, Talwar, Keval Krishan, Dinda, Amit, Joshi, Sujata, Bansal, Pankaj, Saxena, Amit, Arya, Dharamvir Singh·2004·Molecular and Cellular Biochemistry
DOI 10.1023/b:mcbi.0000026051.16803.03
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Abstract

The efficacy of Withania somnifera (Ws) to limit myocardial injury after ischemia and reperfusion was explored and compared to that of Vit E, a reference standard known to reduce mortality and infarct size due to myocardial infarction. Wistar rats (150-200 g) were divided into six groups and received orally saline (sham, control group), Ws-50/kg (Ws control and treated group) and Vit E-100 mg/kg (Vit E control and treated group) respectively for 1 month. On the 31st day, rats of the control, Vit E and Ws treated groups were anesthetized and subjected to 45 min occlusion of the LAD coronary artery followed by 60 min reperfusion. Hemodynamic parameters: systolic, diastolic and mean arterial pressure (SAP, DAP, MAP), heart rate (HR), left ventricular end diastolic pressure (LVEDP), left ventricular peak (+)LVdP/dt and (-)LVdP/dt were monitored. Hearts were removed and processed for histopathological and biochemical studies: Myocardial enzyme viz, creatin phosphokinase (CPK), and antioxidant parameters: malondialdehyde (MDA), glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSHPx) were estimated. Postischemic reperfusion produced significant cardiac necrosis, depression of left ventricular functions (MAP, LVEDP, (+) and (-)LVdP/dt) and a significant fall in GSH (p < 0.01), SOD, CAT (p < 0.05), LDH and CPK (p < 0.01) as well as an increase in MDA level (p < 0.05) in the control group rats as compared to sham group. The changes in levels of protein and GPx was however, not significant. Ws and Vit E favorably modulated most of the hemodynamic, biochemical and histopathological parameters though no significant restoration in GSH, MAP (with Vit E) were observed. Ws on chronic administration markedly augmented antioxidants (GSH, GSHPx, SOD, CAT) while Vit E did not stimulate the synthesis of endogenous antioxidants compared to sham. Results indicate that Ws significantly reduced myocardial injury and emphasize the beneficial action of Ws as a cardioprotective agent.

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Authors
Mohanty, I., Gupta, S. K., Talwar, K. K., Dinda, A., Joshi, S., Bansal, P., Saxena, A., & Arya, D. S.
Affiliations
All India Institute of Medical Sciences (IN)
Corresponding author
Gupta, Suresh Kumar
ORCID
Venue
Molecular and Cellular Biochemistry
Publisher
Springer Science and Business Media LLC
Published
2004-05
Volume
260
Issue
1
Pages
39-47
ISSN
  • 0300-8177 (ISSN-L)
  • 1573-4919
DOI
10.1023/b:mcbi.0000026051.16803.03
OpenAlex
W2009549718
Cited by
119
References
24
Citations by year
2026: 2 · 2025: 3 · 2024: 7 · 2023: 2 · 2022: 3 · 2021: 4 · 2020: 5 · 2019: 5 · 2018: 2 · 2017: 5 · 2016: 7 · 2015: 7 · 2014: 8 · 2013: 12 · 2012: 11
FWCI
2.47
Type
journal article
Language
en
Open access
closed
Licence
Locations
  • Molecular and Cellular Biochemistry (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Indian National Science Academy
  • Ministry of Environment
Topics
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Cardiac Ischemia and Reperfusion
  • Health Sciences › Medicine › Complementary and alternative medicine › Phytochemicals and Medicinal Plants
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiac Fibrosis and Remodeling
Keywords
Withania somnifera, myocardial ischemia-reperfusion injury, cardioprotection, vitamin E, antioxidant enzymes, oxidative stress, malondialdehyde, glutathione, superoxide dismutase, catalase, glutathione peroxidase, creatine kinase, left ventricular function, myocardial infarction, coronary artery occlusion
MeSH terms
Animals, Blood Pressure, Catalase, Creatine Kinase, Female, Glutathione, Glutathione Peroxidase, Heart Rate, Isoproterenol, Male, Malondialdehyde, Phytotherapy, Myocardial Infarction, Myocardium, Necrosis, Plant Extracts, Superoxide Dismutase, Vitamin E, Reperfusion Injury, Myocardial Ischemia, Rats, Wistar, Ventricular Pressure, Plant Roots, Withania, Rats

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