CoChat
Sign Up
Cited by 47Oral microbiology and periodontitis researchDental Health and Care UtilizationInfection Control in Healthcare

In vitro evaluation of the retention of three species of pathogenic microorganisms by three different types of toothbrush

Bunetel, L., Tricot‐Doleux, S., Agnani, G., Bonnaure‐Mallet, M.·2000·Oral Microbiology and Immunology
DOI 10.1034/j.1399-302x.2000.150508.x
Open this paper in CoChat — free account

Abstract

The retention and survival of microorganisms on toothbrushes pose a threat of recontamination for certain patients at risk. In order to measure the influence of brush design and optimize the choice of toothbrush model for complementary studies, the in vitro retention of three microbial species (Porphyromonas gingivalis ATCC 33277, Streptococcus mutans ATCC 25175 and Candida albicans ATCC 26555) was evaluated for three types of toothbrush. Two series of standardized experiments were carried out for each brush and microorganism. The first series tested the retention of the microorganisms on the head portion of the brush, while the second measured retention on the head of the brush and the part of the handle inserted in the mouth during brushing. For each series, the microorganisms were counted at T 0 and T 24 (after storage of the brushes at room temperature for 24 h). Depending on the microorganism studied, from 0.2% to 2% of the initial inoculum was retained on the brush. The number detected increased with the size of the exposed area. After 24 h, P. gingivalis and S. mutans were found on only one type of brush. C. albicans survived on all three. These results confirm that microorganisms can quickly colonize toothbrushes.

Integrity signals

Registry

Crossref · 10.1034/j.1399-302x.2000.150508.x

Cites

36 of 36 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

This paper’s full text isn’t openly available, so its claims and self-check weren’t run. The registry and citation checks still apply.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

  • In vitro biofilm culture systems using human saliva or defined media have served as a useful surrogate for oral biofilm research and have shed light on mechanisms involved in microbial adherence, species interactions, antibiofilm treatments, and organization of the microbial community.

    “In vitro biofilm culture systems using human saliva or defined media have served as a useful surrogate for oral biofilm research and have shed light on the mechanisms involved in microbial adherence, species interactions, antibiofilm treatments, and organization of the microbial community.55, 56, 57, 58, 59”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [55]

  • Previous studies have demonstrated that toothbrushes can inoculate the oral cavity with microorganisms, but there has been little research on how microbes harbored on the toothbrush influence the oral microbiota as a whole.

    “Previous studies have demonstrated that toothbrushes can inoculate the oral cavity with microorganisms, but there has been little research on how the microbes harbored on the toothbrush influence the oral microbiota as a whole.”

    From The oral microbiome: Role of key organisms and complex networks in oral health and disease (2021) · cites this paper as [55]

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Bunetel, L., Tricot‐Doleux, S., Agnani, G., & Bonnaure‐Mallet, M.
Affiliations
Université Rennes 1 (FR)
Corresponding author
Bunetel, L.
ORCID
Bonnaure‐Mallet, M.
Venue
Oral Microbiology and Immunology
Publisher
Wiley
Published
2000-10
Volume
15
Issue
5
Pages
313-316
ISSN
  • 0902-0055 (ISSN-L)
  • 1399-302X
DOI
10.1034/j.1399-302x.2000.150508.x
OpenAlex
W2123469749
Cited by
47
References
36
Citations by year
2026: 1 · 2025: 2 · 2023: 6 · 2022: 4 · 2021: 3 · 2020: 2 · 2019: 2 · 2018: 1 · 2016: 1 · 2015: 1 · 2014: 5 · 2013: 4 · 2012: 5
FWCI
0.45
Type
journal article
Language
en
Open access
closed
Licence
onlinelibrary.wiley.com/termsAndConditions#vor (vor, 2930 d)
Locations
  • Oral Microbiology and Immunology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Health Sciences › Dentistry › Periodontics › Oral microbiology and periodontitis research
  • Health Sciences › Dentistry › Periodontics › Dental Health and Care Utilization
  • Health Sciences › Medicine › Infectious Diseases › Infection Control in Healthcare
Keywords
toothbrush design, recontamination, Porphyromonas gingivalis, Streptococcus mutans, Candida albicans, microbial survival, oral care products
MeSH terms
Candida albicans, Equipment Contamination, Equipment Design, Humans, Risk Factors, Statistics as Topic, Streptococcus mutans, Surface Properties, Temperature, Time Factors, Toothbrushing, Colony Count, Microbial, Porphyromonas gingivalis

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review