Pea rms6 mutants exhibit increased basal branching

Abstract

Our studies on two branching mutants of pea (Pisum sativum L.) have identified a further Ramosus locus, Rms6, with two recessive or partially recessive mutant alleles: rms6‐1 (type line S2‐271) and rms6‐2 (type line K586). Mutants rms6‐1 and rms6‐2 were derived from dwarf and tall cultivars, Solara and Torsdag, respectively. The rms6 mutants are characterized by increased branching from basal nodes. In contrast, mutants rms1 through rms5 have increased branching from both basal and aerial (upper stem) nodes. Buds at the cotyledonary node of wild‐type (WT) plants remain dormant but in rms6 plants these buds were usually released from dormancy. Their growth was either subsequently inhibited, sometimes even prior to emergence above ground, or they grew into secondary stems. The mutant phenotype was strongest for rms6‐1 on the dwarf background. Although rms6‐2 had a weak single‐mutant phenotype, the rms3‐1 rms6‐2 double mutant showed clear transgression and an additive branching phenotype, with a total lateral length almost 2‐fold greater than rms3‐1 and nearly 5‐fold greater than rms6‐2. Grafting studies between WT and rms6‐1 plants demonstrated the primary action of Rms6 may be confined to the shoot. Young WT and rms6‐1 shoots had similar auxin levels, and decapitated plants had a similar magnitude of response to applied auxin. Abscisic acid levels were elevated 2‐fold at node 2 of young rms6‐1 plants. The Rms6 locus mapped to the R to Gp segment of linkage group V (chromosome 3). The rms6 mutants will be useful for basic research and also have possible agronomical value.