The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Autophosphorylation sites on the epidermal growth factor receptor
Abstract
The epidermal growth factor (EGF) receptor is a tyrosine-specific protein kinase with autophosphorylating activity. A 300 amino acid-long region of the receptor's cytoplasmic domain matches (35-90% homology) sequences of transforming proteins from the src family and includes a putative nucleotide binding site. Several of the src transforming proteins have tyrosine kinase activity, but v-erb-B, which appears to be a truncated EGF receptor, is virtually identical to the receptor over this region and yet lacks detectable kinase activity. To locate possible acceptor sites in the v-erb-B protein, we have mapped these sites in the human EGF receptor. We report here that three tyrosine sites near the C-terminus are phosphorylated in vitro. In intact cells, we find that EGF stimulates phosphorylation of several sites, the tyrosine 14 residues from the C-terminus being modified the most extensively. The equivalent site is absent in the v-erb-B protein of avian erythroblastosis virus (AEV) and may influence tyrosine kinase activity.
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“First, we observed that POS phagocytosis induced activation of EGFR (p-EGFR), as indicated by phosphorylation of its tyrosine residue 1068 (Tyr1068) (Fig. 6A), a major autophosphorylation site of the receptor.36”
From RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye (2017) · cites this paper as [36]
In the citing paper
“a major autophosphorylation site of the receptor.36”
From RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye (2017) · cites this paper as [36]
Citations
Cites
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Self-citationClose similarity of epidermal growth factor receptor and v-erb-B oncogene protein sequences
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A native 170,000 epidermal growth factor receptor-kinase complex from shed plasma membrane vesicles.
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Epidermal growth factor induces rapid tyrosine phosphorylation of proteins in A431 human tumor cells
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The erbB gene of avian erythroblastosis virus is a member of the src gene family
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Analysis of the sequence of amino acids surrounding sites of tyrosine phosphorylation.
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The product of the avian erythroblastosis virus erbB locus is a glycoprotein
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Flagged first, then by citations
Research works printed but not linked: 2
- Waterfield, M. D., Scrace, G. & Totty, N. in Practical Protein Biochemistry (ed. Darbre, A.) (Wiley, New York, in the press).
- Hunter, T. & Cooper, J. A. UCLA Symp. molec. cell. Biol. 6, 360 (1983).
Cited by
Sorted by citations
The ErbB signaling network: receptor heterodimerization in development and cancer
Olayioye, M. A., Neve, R. M., Lane, H. A., & Hynes, N. E. (2000) · The EMBO Journal · doi:10.1093/emboj/19.13.3159 · Cited by 2,489
Human insulin receptor and its relationship to the tyrosine kinase family of oncogenes
Ullrich, A., Bell, J. R., Chen, E. Y., Herrera, R., Petruzzelli, L., Dull, T. J., Gray, A., Coussens, L. M., Liao, Y.-C., Tsubokawa, M., et al. (1985) · Nature · doi:10.1038/313756a0 · Cited by 2,251
Coussens, L. M., Yang‐Feng, T. L., Liao, Y.-C., Chen, E., Gray, A., McGrath, J. P., Seeburg, P. H., Libermann, T. A., Schlessinger, J., Francke, U., et al. (1985) · Science · doi:10.1126/science.2999974 · Cited by 1,815
Carpenter, G., & Cohen, S. (1990) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)38983-5 · Cited by 1,626
Human proto‐oncogene c‐kit: a new cell surface receptor tyrosine kinase for an unidentified ligand.
Yarden, Y., Kuang, W.-J., Yang‐Feng, T. L., Coussens, L. M., Munemitsu, S. M., Dull, T. J., Chen, E., Schlessinger, J., Francke, U., & Ullrich, A. (1987) · The EMBO Journal · doi:10.1002/j.1460-2075.1987.tb02655.x · Cited by 1,573
Metadata
- Authors
- Downward, J., Parker, P., & Waterfield, M. D.
- Venue
- Nature
- Published
- 1984-10
- Volume
- 311
- Issue
- 5985
- Pages
- 483-485
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2056681493
- Cited by
- 704
- References
- 22
- Citations by year
- 2026: 2 · 2025: 7 · 2024: 5 · 2023: 5 · 2022: 11 · 2021: 14 · 2020: 9 · 2019: 9 · 2018: 11 · 2017: 16 · 2016: 15 · 2015: 13 · 2014: 17 · 2013: 18 · 2012: 15
- FWCI
- 22.58
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Health Sciences › Medicine › Oncology › HER2/EGFR in Cancer Research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Protein Kinase Regulation and GTPase Signaling
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Lung Cancer Treatments and Mutations
- Keywords
- epidermal growth factor receptor, autophosphorylation, tyrosine kinase, autophosphorylation sites, avian erythroblastosis virus, receptor cytoplasmic domain, kinase activity, transforming protein
- MeSH terms
- Amino Acid Sequence, Epidermal Growth Factor, Humans, Phosphorylation, Receptors, Cell Surface, Tyrosine, ErbB Receptors
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