Reproducibility: Standardize antibodies used in research

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Items 101–200 of 640

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   Jeffrey R. Whiteaker, Lei Zhao, Ping Yan, Richard G. Ivey, Uliana J. Voytovich, Heather D. Moore, Chenwei Lin, Amanda G. Paulovich · 2015 · Molecular & Cellular Proteomics
2. Evaluation of the selectivity and sensitivity of isoform- and mutation-specific RAS antibodies
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3. Selective Sensing of Phosphorylated Peptides and Monitoring Kinase and Phosphatase Activity with a Supramolecular Tandem Assay
   Yang Liu, Jiwon Lee, Lizeth Perez, Adam D. Gill, Richard James Hooley, Wenwan Zhong · 2018 · Journal of the American Chemical Society
4. Cell type-specific roles of NLRP3, inflammasome-dependent and -independent, in host defense, sterile necroinflammation, tissue repair, and fibrosis
   Tamisa Seeko Bandeira Honda, John Ku, Hans‐Joachim Anders · 2023 · Frontiers in Immunology
5. The apelinergic system: a perspective on challenges and opportunities in cardiovascular and metabolic disorders
   Éric Marsault, Catherine Llorens‐Cortés, Xavier Iturrioz, Hyung J. Chun, Olivier Lesur, Gavin Y. Oudit, Mannix Auger‐Messier · 2019 · Annals of the New York Academy of Sciences
6. The multiplicity of analysis strategies jeopardizes replicability: lessons learned across disciplines
   Sabine Hoffmann, F. Schönbrodt, Ralf Elsas, Rory Wilson, U. Strasser, A. Boulesteix · 2020 · Royal Society Open Science
7. The European antibody network's practical guide to finding and validating suitable antibodies for research
   Giovanna Roncador, Pablo Engel, Lorena Maestre, Amanda P. Anderson, Jacqueline Cordell, Mark Steven Cragg, Vladka Čurin Šerbec, Margaret Jones, Vanda Juranić Lisnić, Leonor Kremer et al. · 2015 · mAbs
8. Augmenting adaptive immunity: progress and challenges in the quantitative engineering and analysis of adaptive immune receptor repertoires
   Brown, Alex J., Snapkov, Igor, Akbar, Rahmad, Pavlović, Milena, Miho, Enkelejda, Sandve, Geir K., Greiff, Victor · 2019 · Molecular Systems Design & Engineering
9. Affinity Proteomics to Study Endogenous Protein Complexes: Pointers, Pitfalls, Preferences and Perspectives
   John LaCava, Kelly R. Molloy, Martin S. Taylor, Michal Domanski, Brian T. Chait, Michael P. Rout · 2015 · BioTechniques
10. An in silico method to assess antibody fragment polyreactivity
    Edward P. Harvey, Jung-Eun Shin, Meredith A. Skiba, Genevieve R. Nemeth, Joseph D. Hurley, Alon Wellner, Ada Y. Shaw, Victor G. Miranda, Joseph Min, Chang C. Liu et al. · 2022 · Nature Communications
11. Strategies for Selecting Membrane Protein-Specific Antibodies using Phage Display with Cell-Based Panning
    Mohamed A. Alfaleh, Martina L. Jones, Christopher B. Howard, Stephen M. Mahler · 2017 · Antibodies
12. Getting to reproducible antibodies: the rationale for sequenced recombinant characterized reagents
    Andrew M. Bradbury, Andreas Plückthun · 2015 · Protein Engineering Design and Selection
13. YCharOS open antibody characterisation data: Lessons learned and progress made
    Michael Biddle, Harvinder S Virk · 2023 · F1000Research
14. The Antibody Society’s antibody validation webinar series
    Jan L. A. Voskuil, Anita E. Bandrowski, C. Glenn Begley, Andrew M. Bradbury, Andrew D. Chalmers, Aldrin V. Gomes, Travis Hardcastle, Fridtjof Lund‐Johansen, Andreas Plückthun, Giovanna Roncador et al. · 2020 · mAbs
15. Antibody validation: a view from the mountains
    Michael Taussig, Cláudia Fonseca, James S. Trimmer · 2018 · New Biotechnology
16. Scaling of an antibody validation procedure enables quantification of antibody performance in major research applications
    Ayoubi, Riham, Ryan, Joel, Biddle, Michael S, Alshafie, Walaa, Fotouhi, Maryam, Bolivar, Sara Gonzalez, Ruiz Moleon, Vera, Eckmann, Peter, Worrall, Donovan, McDowell, Ian et al. · 2023 · eLife
17. PharmacoSTORM nanoscale pharmacology reveals cariprazine binding on Islands of Calleja granule cells
    Susanne Prokop, Péter Ábrányi‐Balogh, Benjámin Barti, Márton Vámosi, Miklós Zöldi, Laszlo Barna, Gabriella M. Urbán, András Dávid Tóth, Barna Dudok, Attila Egyed et al. · 2021 · Nature Communications
18. Recombinant antibody production evolves into multiple options aimed at yielding reagents suitable for application-specific needs
    Ario de Marco · 2015 · Microbial Cell Factories
19. A switchable yeast display/secretion system
    James A. Van Deventer, Ryan L. Kelly, Saravanan Rajan, Karl Dane Wittrup, Sachdev S. Sidhu · 2015 · Protein Engineering Design and Selection
20. Specific Noncompetitive Immunoassay for HT-2 Mycotoxin Detection
    Henri O. Arola, Antti Tullila, Harri Kiljunen, Katrina Campbell, Harri Siitari, Tarja K. Nevanen · 2016 · Analytical Chemistry
21. Isolation of anti-extra-cellular vesicle single-domain antibodies by direct panning on vesicle-enriched fractions
    Milica Popović, Elisa Mazzega, Barbara Toffoletto, Ario de Marco · 2018 · Microbial Cell Factories
22. Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer
    Lidija Filipović, M. Spasojević, Radivoje Prodanović, Aleksandra Korać, S Matijasević, Goran N. Brajušković, Ario de Marco, Milica Popović · 2022 · New Biotechnology
23. Animal‐friendly affinity reagents: replacing the needless in the haystack
    Gray, A.C., Sidhu, S.S., Chandrasekera, P.C., Hendriksen, C.F.M., Borrebaeck, C.A.K. · 2016 · Trends in Biotechnology
24. A modular approach for assembling turn-on fluorescence sensors using molecularly imprinted nanoparticles
    Qianjin Li, Tripta Kamra, Lei Ye · 2016 · Chemical Communications
25. Multifunctional Screening Platform for the Highly Efficient Discovery of Aptamers with High Affinity and Specificity
    Shao-Li Hong, Ya-Tao Wan, Man Tang, Dai‐Wen Pang, Zhiling Zhang · 2017 · Analytical Chemistry
26. Nanobodies reveal an extra-synaptic population of SNAP-25 and Syntaxin 1A in hippocampal neurons
    Manuel Maidorn, Aurélien Olichon, Silvio O. Rizzoli, Felipe Opazo · 2018 · mAbs
27. Nonlytic Recombinant Phage Tail Fiber Protein for Specific Recognition of Pseudomonas aeruginosa
    Yong Qin He, Yanli Shi, Mengli Liu, Yingran Wang, Lin Wang, Shuguang Lu, Zhifeng Fu · 2018 · Analytical Chemistry
28. A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain
    Nicolas P. Andrews, Justin X. Boeckman, Colleen F. Manning, Joe Truong Nguyen, Hannah Bechtold, Camelia Dumitras, Belvin Gong, Kimberly Nguyen, Deborah A. van der List, Karl D. Murray et al. · 2019 · eLife
29. Antibodies and methods for immunohistochemistry of extracellular matrix proteins
    Steffen Rickelt, Richard O. Hynes · 2018 · Matrix Biology
30. Non-antibody protein-based biosensors
    Paul Ko Ferrigno · 2016 · Essays in Biochemistry
31. Tools and limitations to study the molecular composition of synapses by fluorescence microscopy
    Manuel Maidorn, Silvio O. Rizzoli, Felipe Opazo · 2016 · Biochemical Journal
32. Development of a structure-switching aptamer-based nanosensor for salicylic acid detection
    Changtian Chen, Silu Feng, Mian Zhou, Chonghui Ji, Long Que, Wei Wang · 2019 · Biosensors and Bioelectronics
33. Alternative reagents to antibodies in imaging applications
    Robert Bedford, Christian Tiede, Ruth Hughes, Alistair Curd, Michael J. McPherson, Michelle Peckham, Darren Charles Tomlinson · 2017 · Biophysical Reviews
34. Highly multiplexed and quantitative cell-surface protein profiling using genetically barcoded antibodies
    Samuel B. Pollock, Amy Hu, Yun Mou, Alexander J. Martinko, Olivier Julien, Michael J. Hornsby, Lynda Ploder, Jarrett Adams, Huimin Geng, Markus Müschen et al. · 2018 · Proceedings of the National Academy of Sciences
35. Next-generation antibodies for post-translational modifications
    Takamitsu Hattori, Shohei Koide · 2018 · Current Opinion in Structural Biology
36. Aptamers as Therapeutic Agents: Has the Initial Euphoria Subsided?
    Silvana K. Haßel, Günter Mayer · 2019 · Molecular Diagnosis & Therapy
37. Quality control of protein reagents for the improvement of research data reproducibility
    Ario de Marco, Nicholas Simon Berrow, Mario Lebendiker, María García-Alai, Stefan H. Knauer, Blanca Lopez Mendez, André Matagne, Annabel H. A. Parret, Kim Remans, Stephan F. W. Uebel et al. · 2021 · Nature Communications
38. Combining Excellent Selectivity with Broad Target Scope: Biosensing with Arrayed Deep Cavitand Hosts
    Wenwan Zhong, Richard James Hooley · 2022 · Accounts of Chemical Research
39. Immunogenomic engineering of a plug-and-(dis)play hybridoma platform
    Mark Pogson, Cristina Parola, William Kelton, Paul Heuberger, Sai T. Reddy · 2016 · Nature Communications
40. A critical evaluation of some of the recent so-called ‘evidence’ for the involvement of vertebrate-type sex steroids in the reproduction of mollusks
    Istvan Fodor, Peter Paul Urban, Alexander P. Scott, Zsolt Pirger · 2020 · Molecular and Cellular Endocrinology
41. Aptamers: an emerging class of bioaffinity ligands in bioactive peptide applications
    Caleb Acquah, Dominic Agyei, Eugene Marfo Obeng, Sharadwata Pan, Kei Xian Tan, Michael K. Danquah · 2019 · Critical Reviews in Food Science and Nutrition
42. A Framework for Improving the Quality of Research in the Biological Sciences
    Casadevall, Arturo, Ellis, Lee M., Davies, Erika W., McFall-Ngai, Margaret, Fang, Ferric C. · 2016 · mBio
43. Mitochondrial CDP-diacylglycerol synthase activity is due to the peripheral protein, TAMM41 and not due to the integral membrane protein, CDP-diacylglycerol synthase 1
    Nicholas J. Blunsom, Evelyn Gomez-Espinosa, Tim G. Ashlin, Shamshad Cockcroft · 2017 · Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids
44. The antibody horror show: an introductory guide for the perplexed
    Simon L. Goodman · 2018 · New Biotechnology
45. Quality and cost assessment of a recombinant antibody fragment produced from mammalian, yeast and prokaryotic host cells: A case study prior to pharmaceutical development
    Kristell Lebozec, Martine Jandrot‐Perrus, Gilles Avenard, Olivier Favre‐Bulle, Philippe Billiald · 2018 · New Biotechnology
46. Optimization of an enzyme linked DNA aptamer assay for cardiac troponin I detection: synchronous multiple sample analysis on an integrated microfluidic platform
    Priya Gopinathan, Anirban Sinha, Yi-Da Chung, Shu‐Chu Shiesh, Gwo‐Bin Lee · 2019 · The Analyst
47. Exploring the role of polymers to overcome ongoing challenges in the field of extracellular vesicles
    Laurianne Simon, Vincent Lapinte, Marie Morille · 2023 · Journal of Extracellular Vesicles
48. Evaluation of milk cathelicidin for detection of dairy sheep mastitis
    María Filippa Addis, Vittorio Tedde, Simone Dore, Salvatore Pisanu, G. Puggioni, Anna Maria Roggio, Daniela Pagnozzi, Stefano A. Lollai, Eugenia Agnese Cannas, Sergio Uzzau · 2016 · Journal of Dairy Science
49. Mining Naïve Rabbit Antibody Repertoires by Phage Display for Monoclonal Antibodies of Therapeutic Utility
    Haiyong Peng, Thomas Nerreter, Chang Jing, Junpeng Qi, Xiuling Li, Pabalu P. Karunadharma, Gustavo Martínez, Mohammad Fallahi, Jo Soden, Jim Freeth et al. · 2017 · Journal of Molecular Biology
50. On-line Coupling of Aptamer Affinity Solid-Phase Extraction and Immobilized Enzyme Microreactor Capillary Electrophoresis-Mass Spectrometry for the Sensitive Targeted Bottom-Up Analysis of Protein Biomarkers
    Hiba Salim, R. Pero-Gascon, E. Giménez, Fernando Benavente · 2022 · Analytical Chemistry
51. Non-Invasive Delivery of Therapeutics into the Brain: The Potential of Aptamers for Targeted Delivery
    Bakhtiar A. Bukari, Rasika M. Samarasinghe, Jinjutha Noibanchong, Sarah L. Shigdar · 2020 · Biomedicines
52. Molecularly Imprinted Nanobeacons Redirect Innate Immune Killing towards Triple Negative Breast Cancer
    Peixin Guan, Zhanchen Guo, Shuxin Xu, LU Hai-feng, Lisheng Wang, Zikuan Gu, Zhen Liu · 2023 · Angewandte Chemie International Edition
53. Multifunctional biosensor constructed by Ag-coating magnetic-assisted unique urchin core porous shell structure for dual SERS enhancement, enrichment, and quantitative detection of multi-components inflammatory markers
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54. Proteomics approaches to characterize the immune responses in cancer
    Víctor Urbiola-Salvador, Dominika Miroszewska, Agnieszka Jabłońska, Talha Qureshi, Zhi Jane Chen · 2022 · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research
55. An Image Analysis Solution For Quantification and Determination of Immunohistochemistry Staining Reproducibility
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56. Thy1-Targeted Microbubbles for Ultrasound Molecular Imaging of Pancreatic Ductal Adenocarcinoma
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57. Unstable correspondence between salivary testosterone measured with enzyme immunoassays and tandem mass spectrometry
    Prasad, Smrithi, Lassetter, Bethany, Welker, Keith M., Mehta, Pranjal H. · 2019 · Psychoneuroendocrinology
58. The source of matrix-degrading enzymes in human cancer: Problems of research reproducibility and possible solutions
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59. Guidelines for manuscript submission in the peer-reviewed pharmacological literature
    Kevin Mullane, S.J. Enna, Jacques G. Piette, Michael J. Williams · 2015 · Biochemical Pharmacology
60. Generation and analyses of human synthetic antibody libraries and their application for protein microarrays
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61. A high-throughput pipeline for validation of antibodies
    Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Kumar Thakor, Simon Kling, Tomáš Kalina, Tuula A. Nyman, Maria Stensland, Wei Zhou, Gustavo A. de Souza et al. · 2018 · Nature Methods
62. Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq
    Michele Busby, Catherine Xue, Catherine Li, Yossi Farjoun, Elizabeth Gienger, Ido Yofe, Adrianne D. Gladden, Charles B. Epstein, Evan M. Cornett, Scott B. Rothbart et al. · 2016 · Epigenetics & Chromatin
63. Animal-derived-antibody generation faces strict reform in accordance with European Union policy on animal use
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64. Imaging the future: the emerging era of single‐cell spatial proteomics
    Indranil Paul, Carl A. White, Isabella Turcinovic, Andrew Emili · 2020 · FEBS Journal
65. Assessment of Therapeutic Antibody Developability by Combinations of In Vitro and In Silico Methods
    Adriana-Michelle Wolf Pérez, Nikolai Lorenzen, Michele Vendruscolo, Pietro Sormanni · 2021 · Methods in molecular biology
66. Unknown unknowns in biomedical research: does an inability to deal with ambiguity contribute to issues of irreproducibility?
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67. Genome-wide chemical mapping of O-GlcNAcylated proteins in Drosophila melanogaster
    Ta-Wei Liu, Mike Myschyshyn, Donald A. R. Sinclair, Samy Cecioni, Kevin Beja, Barry M. Honda, Ryan D. Morin, David J. Vocadlo · 2016 · Nature Chemical Biology
68. Aptamer-Mediated Delivery and Cell-Targeting Aptamers: Room for Improvement
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69. A recombinant antibody against Plasmodium vivax UIS4 for distinguishing replicating from dormant liver stages
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70. VIPER is a genetically encoded peptide tag for fluorescence and electron microscopy
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71. Marking vertebrates langerhans cells, from fish to mammals
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72. A microfluidic approach for rapid and continuous synthesis of glycoprotein-imprinted nanospheres
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73. The ABCs of finding a good antibody: How to find a good antibody, validate it, and publish meaningful data
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74. JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states
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75. Synthesis of highly selective molecularly imprinted nanoparticles by a solid-phase imprinting strategy for fluorescence turn-on recognition of phospholipid
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76. The antibodies don’t work! The race to rid labs of molecules that ruin experiments
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77. Quantitative mapping of specific proteins in biological tissues by laser ablation–ICP-MS using exogenous labels: aspects to be considered
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78. New Views of Old Proteins: Clarifying the Enigmatic Proteome
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79. Phage long tail fiber protein-immobilized magnetic nanoparticles for rapid and ultrasensitive detection of Salmonella
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80. A consensus platform for antibody characterization
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81. Less is more: low expression of MT1-MMP is optimal to promote migration and tumourigenesis of breast cancer cells
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82. Determination of equilibrium dissociation constants for recombinant antibodies by high-throughput affinity electrophoresis
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83. Antibodies from plants for bionanomaterials
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84. Mechanism-based biomarker discovery
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85. ACE2 protein expression within isogenic cell lines is heterogeneous and associated with distinct transcriptomes
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86. Simultaneous Quantification of Protein Expression and Modifications by Top-down Targeted Proteomics: A Case of the Sarcomeric Subproteome
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87. [Letter to the Editor] The Need for Improved Education and Training in Research Antibody Usage and Validation Practices
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88. Affinity maturation, humanization, and co-crystallization of a rabbit anti-human ROR2 monoclonal antibody for therapeutic applications
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89. Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma patient‐derived xenografts
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90. Recent advances in immunodiagnostics based on biosensor technologies—from central laboratory to the point of care
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91. Non-competitive cyclic peptides for targeting enzyme–substrate complexes
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92. A novel selection strategy for antibody producing hybridoma cells based on a new transgenic fusion cell line
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93. Dispersive solid-phase imprinting of proteins for the production of plastic antibodies
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94. Amplification-Free SARS-CoV-2 Detection Using Nanoyeast-scFv and Ultrasensitive Plasmonic Nanobox-Integrated Nanomixing Microassay
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95. Advances in microfluidic analysis of residual antibiotics in food
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96. A Fluorescent Probe to Unravel Functional Features of Cannabinoid Receptor CB1 in Human Blood and Tonsil Immune System Cells
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97. Antibody characterization is critical to enhance reproducibility in biomedical research
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98. Proteome microarray technology and application: higher, wider, and deeper
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99. Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies
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100. Investigation of Anti-SOD1 Antibodies Yields New Structural Insight into SOD1 Misfolding and Surprising Behavior of the Antibodies Themselves
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