The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells.
Abstract
A soybean cDNA encoding the small subunit peptide of a cotyledon-specific 2S albumin (Gm2S-1) is thought to play a role in arresting mitosis during the DNA endoreduplication and cell expansion phase of seed development. The peptide (termed lunasin) contains the cell adhesion motif Arg-Gly-Asp (RGD) followed by eight aspartic acid residues at its C-terminal end. A chimeric gene encoding the lunasin peptide tagged with green fluorescent protein (GFP) arrested cell division, caused abnormal spindle fiber elongation, chromosomal fragmentation, and cell lysis when transiently transfected into murine embryo fibroblast, murine hepatoma, and human breast cancer cells. Deletion of the polyaspartyl end abolished the antimitotic effect. Subcellular localization of lunasin and immunobinding assay using synthetic peptides revealed the preferential adherence of lunasin to chromatin. Immunofluorescence showed that kinetochore proteins were displaced from the centromere in lunasin-transfected cells. These observations suggest that lunasin binds to the chromatin, leading to disruption of kinetochore formation and inhibition of mitosis.
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“some sequences such as Arg-Gly-Asp-Asp-Asp-Asp-Asp-Asp-Asp-Asp-Asp can have anti-proliferative effects on cancer cells models”
From Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines (2016) · cites this paper as Galvez and de Lumen, 1999
Citations
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Nutritional and Health Benefits of Soy Proteins
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How the sourdough may affect the functional features of leavened baked goods
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Bioactive Molecules Released in Food by Lactic Acid Bacteria: Encrypted Peptides and Biogenic Amines
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Bioactive Peptides in Amaranth (Amaranthus hypochondriacus) Seed
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Metadata
- Authors
- Galvez, A. F., & de Lumen, B. O.
- Affiliations
- University of California, Berkeley (US)
- Venue
- Nature Biotechnology
- Published
- 1999-05
- Volume
- 17
- Issue
- 5
- Pages
- 495-500
- ISSN
- 0733-222X (ISSN-L)
- 1087-0156
- 1546-1696
- DOI
- 10.1038/8676
- OpenAlex
- W1572828491
- Cited by
- 174
- References
- 31
- Citations by year
- 2026: 4 · 2025: 7 · 2024: 6 · 2023: 5 · 2022: 8 · 2021: 7 · 2020: 9 · 2019: 8 · 2018: 7 · 2017: 11 · 2016: 9 · 2015: 8 · 2014: 8 · 2013: 14 · 2012: 6
- FWCI
- 0.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Nature Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Keywords
- lunasin, 2S albumin, soybean, mitosis inhibition, antimitotic activity, centromere, chromosomal fragmentation, seed development
- MeSH terms
- Animals, Cell Division, Cell Line, Chromatin, Flow Cytometry, Humans, Mitosis, Peptides, Plant Proteins, Glycine max, Transfection, DNA, Complementary, Kinetochores, Fluorescent Antibody Technique, Indirect, Soybean Proteins, Mice, DNA Fragmentation
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- Galvez, A. F., & de Lumen, B. O. (1999). A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nature Biotechnology. https://doi.org/10.1038/8676
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
