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Guidelines for the use of cell lines in biomedical research

Geraghty, R J, Capes-Davis, A, Davis, J M, Downward, J, Freshney, R I, Knezevic, I, Lovell-Badge, R, Masters, J R W, Meredith, J, Stacey, G N, Thraves, P, Vias, M·2014·British Journal of Cancer

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Crossref · 10.1038/bjc.2014.166

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  • While some continuous cell lines appear to be stable over long-term passage in vitro, they often undergo substantial genetic changes.

    “While some continuous cell lines appear to be stable over long-term passage in vitro , they often undergo substantial genetic changes (Geraghty et al., 2014).”

    From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Geraghty et al., 2014

    Section 4.4.1 (page unavailable) says the genotype of continuous cell lines can change with time and advises against extended continuous culture. It does not establish that they often undergo substantial changes despite appearing stable over long-term passage. (LLM, conf=0.82)

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Metadata

Authors
Geraghty, R. J., Capes-Davis, A., Davis, J. M., Downward, J., Freshney, R. I., Knezevic, I., Lovell-Badge, R., Masters, J. R. W., Meredith, J., Stacey, G. N., Thraves, P., & Vias, M.
Venue
British Journal of Cancer
Published
2014-08-12
Volume
111
Issue
6
Pages
1021-1046
ISSN
  • 0007-0920
  • 1532-1827
Cited by
464
References
3
Language
en

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