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Guidelines for the use of cell lines in biomedical research
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While some continuous cell lines appear to be stable over long-term passage in vitro, they often undergo substantial genetic changes.
“While some continuous cell lines appear to be stable over long-term passage in vitro , they often undergo substantial genetic changes (Geraghty et al., 2014).”
From Guidance document on Good cell and tissue culture practice 2.0 (GCCP 2.0) (2021) · cites this paper as Geraghty et al., 2014
Section 4.4.1 (page unavailable) says the genotype of continuous cell lines can change with time and advises against extended continuous culture. It does not establish that they often undergo substantial changes despite appearing stable over long-term passage. (LLM, conf=0.82)
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Coecke, S., Balls, M., Bowe, G., Davis, J., Gstraunthaler, G., Hartung, T., Hay, R., Merten, O.-W., Price, A., Schechtman, L., et al. (2005) · Alternatives to Laboratory Animals · doi:10.1177/026119290503300313 · Cited by 371
Self-citationDrexler, H. G., & Uphoff, C. C. (2002) · Cytotechnology · doi:10.1023/a:1022913015916 · Cited by 340
Species identification in cell culture: a two-pronged molecular approach
Cooper, J. K., Sykes, G., King, S., Cottrill, K., Ivanova, N. V., Hanner, R., & Ikonomi, P. (2007) · In Vitro Cellular & Developmental Biology - Animal · doi:10.1007/s11626-007-9060-2 · Cited by 174 · cited 2×
“Periodic audits for evidence of regular maintenance, monitoring and stock control will also help ensure safety and security of storage facilities.”
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Metadata
- Authors
- Geraghty, R. J., Capes-Davis, A., Davis, J. M., Downward, J., Freshney, R. I., Knezevic, I., Lovell-Badge, R., Masters, J. R. W., Meredith, J., Stacey, G. N., Thraves, P., & Vias, M.
- Venue
- British Journal of Cancer
- Published
- 2014-08-12
- Volume
- 111
- Issue
- 6
- Pages
- 1021-1046
- ISSN
- 0007-0920
- 1532-1827
- PMC
- PMC4453835
- Cited by
- 464
- References
- 3
- Language
- en
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