Cell lines authentication and mycoplasma detection as minimun quality control of cell lines in biobanking
Open accessAbstract
Establishment of continuous cell lines from human normal and tumor tissues is an extended and useful methodology for molecular characterization of cancer pathophysiology and drug development in research laboratories. The exchange of these cell lines between different labs is a common practice that can compromise assays reliability due to contamination with microorganism such as mycoplasma or cells from different flasks that compromise experiment reproducibility and reliability. Great proportions of cell lines are contaminated with mycoplasma and/or are replaced by cells derived for a different origin during processing or distribution process. The scientific community has underestimated this problem and thousand of research experiment has been done with cell lines that are incorrectly identified and wrong scientific conclusions have been published. Regular contamination and authentication tests are necessary in order to avoid negative consequences of widespread misidentified and contaminated cell lines. Cell banks generate, store and distribute cell lines for research, being mandatory a consistent and continuous quality program. Methods implementation for guaranteeing both, the absence of mycoplasma and authentication in the supplied cell lines, has been performed in the Andalusian Health System Biobank. Specifically, precise results were obtained using real time PCR detection for mycoplasma and 10 STRs identification by capillary electrophoresis for cell line authentication. Advantages and disadvantages of these protocols are discussed.
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The small size and atypical antibiotic resistance of mycoplasma enable it to pass through filters and multiply in media prodigiously.
“The small size and atypical antibiotic resistance of mycoplasma enable it to pass through filters and multiply in media prodigiously [12].”
From The history, landscape, and outlook of human cell line authentication and security (2024) · cites this paper as [12]
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FAIRsharing record for: Minimal Information for Studies of Extracellular Vesicles
FAIRsharing Team, & Witwer, K. (2018) · FAIRsharing.org · doi:10.25504/fairsharing.z87h0e
- Authors
- Corral-Vázquez, C., Aguilar-Quesada, R., Catalina, P., Lucena-Aguilar, G., Ligero, G., Miranda, B., & Carrillo-Ávila, J. A.
- Affiliations
- Biobanco del Sistema Sanitario Público de Andalucía (ES)
- Corresponding author
- Carrillo-Ávila, J. A.
- Venue
- Cell and Tissue Banking
- Published
- 2017-03-02
- Volume
- 18
- Issue
- 2
- Pages
- 271-280
- ISSN
- 1389-9333 (ISSN-L)
- 1573-6814
- PMC
- PMC5429902
- OpenAlex
- W2592131051
- Cited by
- 47
- References
- 50
- Citations by year
- 2026: 2 · 2025: 5 · 2024: 5 · 2023: 7 · 2022: 5 · 2021: 4 · 2020: 8 · 2019: 6 · 2018: 4 · 2017: 1
- FWCI
- 4.43
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Locations
- Cell and Tissue Banking (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- RISalud Institutional Health Repository of Andalucía (submittedVersion, cc-by)
- Funders
- Instituto de Salud Carlos III (IBS-PT13/0010/0050, IBS-PT130010040)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Microbial infections and disease research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Viral Infectious Diseases and Gene Expression in Insects
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
- Keywords
- cell line authentication, mycoplasma detection, cell line contamination, biobanking, quality control, short tandem repeat profiling, real-time PCR, capillary electrophoresis, cell line misidentification
- MeSH terms
- Cell Line, DNA, Bacterial, Humans, Mycoplasma, Mycoplasma Infections, Quality Control, Polymerase Chain Reaction, Biological Specimen Banks, Cell Culture Techniques
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