Cited by 211Cell death mechanisms and regulationAutophagy in Disease and TherapyFerroptosis and cancer prognosis

The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.

Obatoclax (GX15-070) triggers necroptosis by promoting the assembly of the necrosome on autophagosomal membranes

Open access

Basit, F, Cristofanon, S, Fulda, S·2013·Cell Death & Differentiation

DOI 10.1038/cdd.2013.45PMC PMC3741498

Open this paper in CoChat — free account Open PDF

Abstract

Obatoclax (GX15-070), a small-molecule inhibitor of antiapoptotic Bcl-2 proteins, has been reported to trigger cell death via autophagy. However, the underlying molecular mechanisms have remained elusive. Here, we identify GX15-070-stimulated assembly of the necrosome on autophagosomal membranes as a key event that connects GX15-070-stimulated autophagy to necroptosis. GX15-070 predominately induces a non-apoptotic form of cell death in rhabdomyosarcoma cells, as evident by lack of typical apoptotic features such as DNA fragmentation or caspase activation and by insensitivity to the broad-range caspase inhibitor zVAD.fmk. Instead, GX15-070 triggers massive accumulation of autophagosomes, which are required for GX15-070-induced cell death, as blockade of autophagosome formation by silencing of Atg5 or Atg7 abolishes GX15-070-mediated cell death. Co-immunoprecipitation studies reveal that GX15-070 stimulates the interaction of Atg5, a constituent of autophagosomal membranes, with components of the necrosome such as FADD, RIP1 and RIP3. This GX15-070-induced assembly of the necrosome on autophagosomes occurs in a Atg5-dependent manner, as knockdown of Atg5 abrogates formation of this complex. RIP1 is necessary for GX15-070-induced cell death, as both genetic and pharmacological inhibition of RIP1 by shRNA-mediated knockdown or by the RIP1 inhibitor necrostatin-1 blocks GX15-070-induced cell death. Similarly, RIP3 knockdown rescues GX15-070-mediated cell death and suppression of clonogenic survival. Interestingly, RIP1 or RIP3 silencing has no effect on GX15-070-stimulated autophagosome formation, underlining that RIP1 and RIP3 mediate cell death downstream of autophagy induction. Of note, GX15-070 significantly suppresses tumor growth in a RIP1-dependent manner in the chorioallantoic membrane model in vivo. In conclusion, GX15-070 triggers necroptosis by promoting the assembly of the necrosome on autophagosomes. These findings provide novel insights into the molecular mechanisms of GX15-070-induced non-apoptotic cell death.

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Integrity signals

Registry

Crossref · 10.1038/cdd.2013.45

Cites

47 of 47 references verified0 citation errors · 0 not checkable

Statistics

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Hallmarks of Cancer: The Next Generation
Hanahan, D., & Weinberg, R. A. (2011) · Cell · doi:10.1016/j.cell.2011.02.013 · Cited by 66,590
Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury
Degterev, A., Huang, Z., Boyce, M., Li, Y., Jagtap, P., Mizushima, N., Cuny, G. D., Mitchison, T. J., Moskowitz, M. A., & Yuan, J. (2005) · Nature Chemical Biology · doi:10.1038/nchembio711 · Cited by 3,068
The Role of Atg Proteins in Autophagosome Formation
Mizushima, N., Yoshimori, T., & Ohsumi, Y. (2011) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-092910-154005 · Cited by 2,978
Molecular definitions of cell death subroutines: recommendations of the Nomenclature Committee on Cell Death 2012
Galluzzi, L., Vitale, I., Abrams, J. M., Alnemri, E. S., Baehrecke, E. H., Blagosklonny, M. V., Dawson, T. M., Dawson, V. L., El-Deiry, W. S., Fulda, S., et al. (2012) · Cell Death & Differentiation · doi:10.1038/cdd.2011.96 · Cited by 2,421
Self-citation
Molecular mechanisms of necroptosis: an ordered cellular explosion
Vandenabeele, P., Galluzzi, L., Vanden Berghe, T., & Kroemer, G. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2970 · Cited by 2,382
The Bcl-2 apoptotic switch in cancer development and therapy
Adams, J. M., & Cory, S. (2007) · Oncogene · doi:10.1038/sj.onc.1210220 · Cited by 2,256
Receptor Interacting Protein Kinase-3 Determines Cellular Necrotic Response to TNF-α
He, S., Wang, L., Miao, L., Wang, T., Du, F., Zhao, L., & Wang, X. (2009) · Cell · doi:10.1016/j.cell.2009.05.021 · Cited by 2,214
Dynamics and diversity in autophagy mechanisms: lessons from yeast
Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2708 · Cited by 1,761
Targeting mitochondria for cancer therapy
Fulda, S., Galluzzi, L., & Kroemer, G. (2010) · Nature Reviews Drug Discovery · doi:10.1038/nrd3137 · Cited by 1,678
Self-citation
Regulation of an ATG7 - beclin 1 Program of Autophagic Cell Death by Caspase-8
Yu, L., Alva, A. S., Su, H. C., Dutt, P., Freundt, E. C., Welsh, S. J., Baehrecke, E. H., & Lenardo, M. J. (2004) · Science · doi:10.1126/science.1096645 · Cited by 1,249

Flagged first, then by citations

Show all 47 references

Sorted by citations

Items 101–200 of 211

The BH3 Mimetic Obatoclax Accumulates in Lysosomes and Causes Their Alkalinization
Stamelos, V. A., Fisher, N. C., Bamrah, H. S., Voisey, C., Price, J. C., Farrell, W. E., Redman, C. W. E., & Richardson, A. (2016) · PLoS ONE · doi:10.1371/journal.pone.0150696 · Cited by 39
The Interplay Between Autophagy and Regulated Necrosis
Zhang, L., Cui, T., & Wang, X. (2022) · Antioxidants and Redox Signaling · doi:10.1089/ars.2022.0110 · Cited by 39
RETRACTED: Recent advances in glioblastoma multiforme therapy: A focus on autophagy regulation
Sanati, M., Binabaj, M. M., Ahmadi, S. S., Aminyavari, S., Javid, H., Mollazadeh, H., Bibak, B., Mohtashami, E., Jamialahmadi, T., Afshari, A. R., et al. (2022) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2022.113740 · Cited by 35
Retracted
Obatoclax impairs lysosomal function to block autophagy in cisplatin-sensitive and -resistant esophageal cancer cells
Yu, L., Wu, W. K. K., Gu, C., Zhong, D., Zhao, X., Kong, Y., Lin, Q., Chan, M. T. V., Zhou, Z., & Liu, S. (2016) · Oncotarget · doi:10.18632/oncotarget.7492 · Cited by 35
OBATOCLAX and ABT-737 Induce ER Stress Responses in Human Melanoma Cells that Limit Induction of Apoptosis
Wroblewski, D., Jiang, C. C., Croft, A. J., Farrelly, M. L., Zhang, X. D., & Hersey, P. (2013) · PLoS ONE · doi:10.1371/journal.pone.0084073 · Cited by 34
Inhibition of HOX/PBX dimer formation leads to necroptosis in acute myeloid leukemia cells
Alharbi, R., Pandha, H., Simpson, G. R., Pettengell, R., Poterlowicz, K., Thompson, A., Harrington, K. J., El‐Tanani, M., & Morgan, R. (2017) · Oncotarget · doi:10.18632/oncotarget.20023 · Cited by 35
Obatoclax and Paclitaxel Synergistically Induce Apoptosis and Overcome Paclitaxel Resistance in Urothelial Cancer Cells
Jiménez‐Guerrero, R., Gasca, J., Flores, M. L., Pérez‐Valderrama, B., Tejera‐Parrado, C., López, R. A. M., Tortolero, M., Romero, F., Japón, M. Á., & Sáez, C. (2018) · Cancers · doi:10.3390/cancers10120490 · Cited by 33
Design, Synthesis, Anticancer Activity, and Solid Lipid Nanoparticle Formulation of Indole- and Benzimidazole-Based Compounds as Pro-Apoptotic Agents Targeting Bcl-2 Protein
Nagy, M., Darwish, K. M., Kishk, S. M., Tantawy, M. A., Nasr, A. M., Qushawy, M. K. E., Swidan, S. A., Mostafa, S. M., & Salama, I. (2021) · Pharmaceuticals · doi:10.3390/ph14020113 · Cited by 33
Altered mitochondrial calcium handling and cell death by necroptosis: An emerging paradigm
Faizan, M. I., & Ahmad, T. (2020) · Mitochondrion · doi:10.1016/j.mito.2020.12.004 · Cited by 31
Essential role for autophagy protein VMP1 in maintaining neuronal homeostasis and preventing axonal degeneration
Wang, P., Chen, X., Wang, Y., Jia, C., Liu, X., Wang, Y., Wu, H., Cai, H., Shen, H., & Le, W. (2021) · Cell Death and Disease · doi:10.1038/s41419-021-03412-5 · Cited by 31
Obatoclax overcomes resistance to cell death in aggressive thyroid carcinomas by countering Bcl2a1 and Mcl1 overexpression
Champa, D., Russo, M. A., Liao, X. H., Refetoff, S., Ghossein, R. A., & Cristofano, A. D. (2014) · Endocrine Related Cancer · doi:10.1530/erc-14-0268 · Cited by 34
Pan-Bcl-2 inhibitor Obatoclax is a potent late stage autophagy inhibitor in colorectal cancer cells independent of canonical autophagy signaling
Koehler, B. C., Jassowicz, A., Scherr, A., Lorenz, S., Radhakrishnan, P., Kautz, N., Elßner, C., Weiß, J., Jaeger, D., Schneider, M., et al. (2015) · BMC Cancer · doi:10.1186/s12885-015-1929-y · Cited by 32
High-Throughput Drug Screen Identifies Chelerythrine as a Selective Inducer of Death in a TSC2-null Setting
Medvetz, D. A., Sun, Y., Li, C., Khabibullin, D., Balan, M., Parkhitko, A. A., Priolo, C., Asara, J. M., Pal, S., Yu, J. J., et al. (2014) · Molecular Cancer Research · doi:10.1158/1541-7786.mcr-14-0440 · Cited by 30
The antipsychotic drug pimozide promotes apoptosis through the RAF/ERK pathway and enhances autophagy in breast cancer cells
Jiang, G., Zhou, X., Hu, Y., Tan, X., Wang, D., Yang, L., Zhang, Q., & Liu, S. (2024) · Cancer Biology & Therapy · doi:10.1080/15384047.2024.2302413 · Cited by 30
Autophagy contributes to modulating the cytotoxicities of Bcl-2 homology domain-3 mimetics
Yu, L., & Liu, S. (2013) · Seminars in Cancer Biology · doi:10.1016/j.semcancer.2013.08.008 · Cited by 31
Differences and Similarities in TRAIL- and Tumor Necrosis Factor-Mediated Necroptotic Signaling in Cancer Cells
Sosna, J., Philipp, S., Chico, J. F., Saggau, C., Fritsch, J., Föll, A., Plenge, J., Arenz, C., Pinkert, T., Kalthoff, H., et al. (2016) · Molecular and Cellular Biology · doi:10.1128/mcb.00941-15 · Cited by 33
Obatoclax kills anaplastic thyroid cancer cells by inducing lysosome neutralization and necrosis
Champa, D., Orlacchio, A., Patel, B., Ranieri, M., Shemetov, A. A., Verkhusha, V. V., Cuervo, A. M., & Cristofano, A. D. (2016) · Oncotarget · doi:10.18632/oncotarget.9121 · Cited by 29
Therapeutic targeting of Bcl-2 family for treatment of B-cell malignancies
Huang, J. Q., Fairbrother, W. J., & Reed, J. C. (2015) · Expert Review of Hematology · doi:10.1586/17474086.2015.1026321 · Cited by 28
Sparse expression bases in cancer reveal tumor drivers
Logsdon, B. A., Gentles, A. J., Miller, C. P., Blau, C. A., Becker, P. S., & Lee, S. (2015) · Nucleic Acids Research · doi:10.1093/nar/gku1290 · Cited by 28
Induction of autophagy by ARHI (DIRAS3) alters fundamental metabolic pathways in ovarian cancer models
Ornelas, A., McCullough, C., Lü, Z., Zacharias, N. M., Kelderhouse, L. E., Gray, J. P., Yang, H., Engel, B. J., Wang, Y., Mao, W., et al. (2016) · BMC Cancer · doi:10.1186/s12885-016-2850-8 · Cited by 27
RIP3 antagonizes a TSC2-mediated pro-survival pathway in glioblastoma cell death
Fettweis, G., Di Valentin, E., L'homme, L., Lassence, C., Dequiedt, F., Fillet, M., Coupienne, I., & Piette, J. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2016.10.014 · Cited by 26
Targeting autophagy with small molecules for cancer therapy
Kondapuram, S. K., Sarvagalla, S., & Coumar, M. S. (2019) · Journal of Cancer Metastasis and Treatment · doi:10.20517/2394-4722.2018.105 · Cited by 29
The Function of Necroptosis and Its Treatment Target in IBD
Akanyibah, F. A., Zhu, Y., Jin, T., Ocansey, D. K. W., Mao, F., & Qiu, W. (2024) · Mediators of Inflammation · doi:10.1155/2024/7275309 · Cited by 27
Obatoclax Induces G1/G0‐Phase Arrest via p38/p21waf1/Cip1 Signaling Pathway in Human Esophageal Cancer Cells
Zhong, D., Gu, C., Shi, L., Xun, T., Li, X., Liu, S., & Yu, L. (2014) · Journal of Cellular Biochemistry · doi:10.1002/jcb.24829 · Cited by 25
Mcl-1 as a “barrier” in cancer treatment: Can we target it now?
Pervushin, N. V., Senichkin, V. V., Zhivotovsky, B., & Kopeina, G. S. (2020) · International review of cytology · doi:10.1016/bs.ircmb.2020.01.002 · Cited by 26
Multi-parametric imaging of cell heterogeneity in apoptosis analysis
Vorobjev, I. A., & Barteneva, N. S. (2016) · Methods · doi:10.1016/j.ymeth.2016.07.003 · Cited by 24
Codelivery of a miR-124 Mimic and Obatoclax by Cholesterol-Penetratin Micelles Simultaneously Induces Apoptosis and Inhibits Autophagic Flux in Breast Cancer in Vitro and in Vivo
Zhang, N., Huang, Y. H., Wu, F., Zhao, Y., Li, X., Shen, P., Yang, L., Luo, Y., Li, Y., & He, G. (2016) · Molecular Pharmaceutics · doi:10.1021/acs.molpharmaceut.6b00211 · Cited by 24
Obatoclax, a Pan-BCL-2 Inhibitor, Targets Cyclin D1 for Degradation to Induce Antiproliferation in Human Colorectal Carcinoma Cells
Or, C.-H. R., Chang, Y., Lin, W.-C., Lee, W.-C., Su, H., Cheung, M.-W., Huang, C.-P., Ho, C., & Chang, C. (2016) · International Journal of Molecular Sciences · doi:10.3390/ijms18010044 · Cited by 24
Targeting autophagy for the treatment of cancer
Fulda, S. (2018) · Biological Chemistry · doi:10.1515/hsz-2018-0105 · Cited by 23
Self-citation
Bulnesia sarmientoi Supercritical Fluid Extract Exhibits Necroptotic Effects and Anti-Metastatic Activity on Lung Cancer Cells
Wang, H.-L., Chang, J.-C., Fang, L.-W., Hsu, H., Lee, L.-C., Yang, J., Liang, M., Hsiao, P.-C., Wang, C.-P., Wang, S., et al. (2018) · Molecules · doi:10.3390/molecules23123304 · Cited by 23
Death by over-eating: The Gaucher disease associated gene GBA1 , identified in a screen for mediators of autophagic cell death, is necessary for developmental cell death in Drosophila midgut
Dasari, S. K., Schejter, E. D., Bialik, S., Shkedy, A., Levin‐Salomon, V., Levin‐Zaidman, S., & Kimchi, A. (2017) · Cell Cycle · doi:10.1080/15384101.2017.1380134 · Cited by 24
Blockade of crizotinib-induced BCL2 elevation in ALK-positive anaplastic large cell lymphoma triggers autophagy associated with cell death
Torossian, A., Broin, N., Frentzel, J., Daugrois, C., Gandarillas, S., Saati, T. A., Lamant, L., Brousset, P., Giuriato, S., & Espinos, E. (2019) · Haematologica · doi:10.3324/haematol.2017.181966 · Cited by 22
The Multifaceted Effects of Autophagy on the Tumor Microenvironment
Kang, R., Zeh, H. J., Lotze, M. T., & Tang, D. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-35727-6_7 · Cited by 22
A Practical Synthesis of Densely Functionalized Pyrroles via a Three-Component Cascade Reaction between Carbohydrates, Oxoacetonitriles, and Ammonium Acetate
Xia, M., Lambu, M. R., Tatina, M. B., & Judeh, Z. M. A. (2020) · The Journal of Organic Chemistry · doi:10.1021/acs.joc.0c02381 · Cited by 22
Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77
Luo, Y., Roy, M., Xiao, X., Sun, S., Liang, L., Chen, H., Fu, Y., Sun, Y., Zhu, M., Ye, M., et al. (2014) · Tumor Biology · doi:10.1007/s13277-014-2924-7 · Cited by 22
Assessing Autophagy in Archived Tissue or How to Capture Autophagic Flux from a Tissue Snapshot
Humbert, M., Morán, M., de la Cruz‐Ojeda, P., Muntané, J., Wiedmer, T., Apostolova, N., McKenna, S. L., Velasco, G., Balduini, W., Eckhart, L., et al. (2020) · Biology · doi:10.3390/biology9030059 · Cited by 23
Acetylshikonin induces necroptosis via the RIPK1/RIPK3-dependent pathway in lung cancer
Lin, S.-S., Chang, T., Wei, A. I., Lee, C., Lin, Z., Chiang, Y., Chi, M., & Liu, J. (2023) · Aging · doi:10.18632/aging.205316 · Cited by 19
Recent advances in the synthesis of pyrroles via multicomponent reactions using arylglyoxals
Javahershenas, R., Arlan, F. M., Prager, R. H., & Khalafy, J. (2020) · ARKIVOC · doi:10.24820/ark.5550190.p011.172 · Cited by 19
Phospho‐ΔNp63α‐responsive microRNAs contribute to the regulation of necroptosis in squamous cell carcinoma upon cisplatin exposure
Ratovitski, E. A. (2015) · FEBS Letters · doi:10.1016/j.febslet.2015.04.020 · Cited by 21
Cell death induction by the BH3 mimetic GX15-070 in thyroid carcinoma cells
Broecker‐Preuss, M., Viehof, J., Jastrow, H., Becher-Boveleth, N., Führer, D., & Mann, K. (2015) · Journal of Experimental & Clinical Cancer Research · doi:10.1186/s13046-015-0186-x · Cited by 18
Regioselective [1N+2C+2C] Assembly of Fully Decorated Pyrroles from Primary Amines, 1,2‐Diaza‐1,3‐dienes, and 2,3‐Allenoates
Perrulli, F. R., Favi, G., Crescentini, L. D., Attanasi, O. A., Santeusanio, S., & Mantellini, F. (2015) · European Journal of Organic Chemistry · doi:10.1002/ejoc.201501017 · Cited by 17
Caspase-1-Dependent Pyroptosis Mediates Adjuvant Activity of Platycodin D as an Adjuvant for Intramuscular Vaccines
Zhu, L., Han, Z., He, Y., & Sun, H. (2022) · Cells · doi:10.3390/cells11010134 · Cited by 17
Exploring beyond Common Cell Death Pathways in Oral Cancer: A Systematic Review
da Rocha, L. D. O. S., de Morais, É. F., de Oliveira, L. Q. R., Barbosa, A. V., Lambert, D., Rocha, C. A. G., & Coletta, R. D. (2024) · Biology · doi:10.3390/biology13020103 · Cited by 14
Proteolytic control of regulated necrosis
Chico, J. F., Saggau, C., & Adam, D. (2017) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2017.05.025 · Cited by 16
Cationic peroxidase from proso millet induces human colon cancer cell necroptosis by regulating autocrine TNF-α and RIPK3 demethylation
Cui, X., Wang, R., & Wang, Z. (2018) · Food & Function · doi:10.1039/c7fo01040k · Cited by 14
Identifying the druggable interactome of EWS-FLI1 reveals MCL-1 dependent differential sensitivities of Ewing sarcoma cells to apoptosis inducers
Tsafou, K., Katschnig, A. M., Radic-Sarikas, B., Mutz, C., Iljin, K., Schwentner, R., Kauer, M. O., Muehlbacher, K., Aryee, D. N. T., Westergaard, D., et al. (2018) · Oncotarget · doi:10.18632/oncotarget.25760 · Cited by 13
Isowalsuranolide targets TrxR1/2 and triggers lysosomal biogenesis and autophagy via the p53-TFEB/TFE3 axis
Xu, Y., Ding, X., Zhao, Y., Wang, Y., Dong, X., Gu, Z., Gu, Z., Fei, J., Zhao, Y., & Hao, X. (2025) · Science China Life Sciences · doi:10.1007/s11427-023-2563-6 · Cited by 12
Effect of autophagy inhibition on cell viability and cell cycle progression in MDA-MB-231 human breast cancer cells
Liu, Q., Shi, X., Zhou, X., Wang, D., Wang, L., & Li, C. L. (2014) · Molecular Medicine Reports · doi:10.3892/mmr.2014.2296 · Cited by 14
Know the enemy as well as the weapons in hand: the aberrant death pathways and therapeutic agents in chronic lymphocytic leukemia.
Huang, Y., Wu, J., Li, J., & Xu, W. (2015) · Europe PMC (PubMed Central) · Cited by 12
The Importance of Being Dead: Cell Death Mechanisms Assessment in Anti-Sarcoma Therapy
Rello‐Varona, S., Herrero‐Martín, D., Lagares‐Tena, L., López‐Alemany, R., Mulet-Margalef, N., Huertas-Martínez, J., Monclús, S. G., del Muro, X. G., Muñoz‐Pinedo, C., & Tirado, Ò. M. (2015) · Frontiers in Oncology · doi:10.3389/fonc.2015.00082 · Cited by 13
Exploitation of necroptosis for treatment of caspase-compromised cancers
Cho, Y. S., & Park, H. L. (2017) · Oncology Letters · doi:10.3892/ol.2017.6285 · Cited by 12
High Levels of miR-7-5p Potentiate Crizotinib-Induced Cytokilling and Autophagic Flux by Targeting RAF1 in NPM-ALK Positive Lymphoma Cells
Sorrentino, D., Frentzel, J., Mitou, G., Blasco, R. B., Torossian, A., Hoareau‐Aveilla, C., Pighi, C., Farcé, M., Meggetto, F., Manenti, S., et al. (2020) · Cancers · doi:10.3390/cancers12102951 · Cited by 13
Programmed Cell Death in the Left and Right Ventricle of the Late Phase of Post-Infarction Heart Failure
Lichý, M., Szobi, A., Hrdlička, J., Neckář, J., Kolář, F., & Adameová, A. (2020) · International Journal of Molecular Sciences · doi:10.3390/ijms21207782 · Cited by 11
New Frontiers in Cancer Chemotherapy — Targeting Cell Death Pathways
Xu, Y. Z., Kanagaratham, C., Youssef, M., & Radzioch, D. (2016) · InTech eBooks · doi:10.5772/61260 · Cited by 12
A caspase-independent way to kill cancer cells
Fitzwalter, B. E., & Thorburn, A. M. (2017) · Nature Cell Biology · doi:10.1038/ncb3604 · Cited by 10
The short life of the Hoyle organ of Sepia officinalis: formation, differentiation and degradation by programmed cell death
Cyran, N., Palumbo, A., Klepal, W., Vidal, É. A. G., Staedler, Y. M., Schönenberger, J., & von Byern, J. (2017) · Hydrobiologia · doi:10.1007/s10750-017-3291-3 · Cited by 9
Physical Exercise and Liver Autophagy: Potential Roles of IL-6 and Irisin
Pinto, A. P., Ropelle, E. R., Quadrilatero, J., & da Silva, A. S. R. (2021) · Exercise and Sport Sciences Reviews · doi:10.1249/jes.0000000000000278 · Cited by 10
FADD-deficient mouse embryonic fibroblasts undergo RIPK1-dependent apoptosis and autophagy after NB-UVB irradiation
Antunović, M., Matić, I., Nagy, B., Mihalić, K. C., Skelin, J., Štambuk, J., Josipović, P., Bender, T., Paradžik, M., & Marijanović, I. (2019) · Journal of Photochemistry and Photobiology B Biology · doi:10.1016/j.jphotobiol.2019.03.007 · Cited by 9
Comparative value of urinalysis, urine cytology and urine sIL2R in the assessment of renal disease in patients with systemic lupus erythematosus (SLE).
Roberti, I., DIKMAN, S. H., Spiera, H., Reisman, L., Eichenfield, A. H., & Lieberman, K. V. (1996) · PubMed · Cited by 9
Pharmaceutical Agents for Targeting Autophagy and Their Applications in Clinics
Kench, U. S., Sologova, S. S., Smolyarchuk, Е. А., Prassolov, V., & Spirin, P. (2024) · Pharmaceuticals · doi:10.3390/ph17101355 · Cited by 9
Necroptosis in inflammatory bowel disease: A potential effective target.
Long, X., Zhu, N., Qiu, J., Yu, X., Ruan, X., Wang, X., & Tian, L. (2022) · PubMed Central · doi:10.11817/j.issn.1672-7347.2022.210501 · Cited by 8
Yunnan baiyao exerts anti-glioma activity by inducing autophagy-dependent necroptosis
Zhang, N., Guo, K., Lin, W., Wang, Z., Fuming, Z., Zhang, X., Zheng, D., & Ma, W. (2024) · Journal of Ethnopharmacology · doi:10.1016/j.jep.2024.118658 · Cited by 8
Determinants for Antitumor and Protumor Effects of Programmed Cell Death
Workenhe, S. T., Inkol, J. M., Westerveld, M. J., Verburg, S. G., Worfolk, S. M., Walsh, S. R., & Kallio, K. L. F. (2023) · Cancer Immunology Research · doi:10.1158/2326-6066.cir-23-0321 · Cited by 7
ROS, Redox Regulation, and Anticancer Therapy
Bansal, M. P. (2023) · Springer eBooks · doi:10.1007/978-981-99-7342-2_9 · Cited by 7
Necroptosis in the sarcoma immune microenvironment: From biology to therapy
Ye, H., Lu, M., Tu, C., & Li, M. (2023) · International Immunopharmacology · doi:10.1016/j.intimp.2023.110603 · Cited by 6
Alcohol induces hepatocytes necroptosis through the LC3/RIPK1/RIPK3 pathway
Chen, Y., Liu, M., Wei, H., Guo, J., Zhang, S., Bu, X., Chen, S., Zhang, D., & Guan, S. (2023) · Food and Chemical Toxicology · doi:10.1016/j.fct.2023.114124 · Cited by 7
Autophagy in Multidrug-Resistant Cancers
Kwan, B. L. Y., & Wai, V. W. K. (2016) · InTech eBooks · doi:10.5772/64274 · Cited by 7
The Dual Role of Autophagy in Crizotinib-Treated ALK+ ALCL: From the Lymphoma Cells Drug Resistance to Their Demise
Espinos, E., Lai, R. L., & Giuriato, S. (2021) · Cells · doi:10.3390/cells10102517 · Cited by 6
Copper–obatoclax derivative complexes mediate DNA cleavage and exhibit anti-cancer effects in hepatocellular carcinoma
Su, J.-C., Chang, J.-H., Huang, J., Chen, P., Chen, K., Tseng, P., & Shiau, C. (2015) · Chemico-Biological Interactions · doi:10.1016/j.cbi.2014.12.026 · Cited by 6
Comparison of in vitro antileukemic activity of obatoclax and ABT-737
Opydo‐Chanek, M., & Mazur, L. (2016) · Tumor Biology · doi:10.1007/s13277-016-4943-z · Cited by 6
Autophagic cell death RIPs into tumors
Oberst, A. (2013) · Cell Death and Differentiation · doi:10.1038/cdd.2013.89 · Cited by 6
Autophagy in erectile dysfunction: focusing on apoptosis and fibrosis
Luo, P., Zou, J., Chen, T., Zou, J., Li, W., Chen, Q., Cheng, L., Zheng, L., & Qian, B. (2024) · Asian Journal of Andrology · doi:10.4103/aja202433 · Cited by 5
The Microenvironment of Tongue Cancer
Tao, W., Li-Juan, Z., Li, K., Jing-yuan, L., Liu, X., & Liang, Y. (2020) · Advances in experimental medicine and biology · doi:10.1007/978-3-030-59038-3_4 · Cited by 5
Efficient synthesis of pentasubstituted pyrroles via intramolecular C-arylation
Lemrová, B., Maloň, M., & Soural, M. (2022) · Organic & Biomolecular Chemistry · doi:10.1039/d2ob00536k · Cited by 5
Arachidonic acid induces pyroptosis via a non-autophagic function of mitophagy and enhances immunotherapy in a PDAC model
Chu, T., Liu, Z., Liu, H., Mu, W., Zhi, Y., Sheng, N., Zhang, J., Peng, X., Lv, S., Duan, K., et al. (2026) · Nature Communications · doi:10.1038/s41467-025-68267-2 · Cited by 5
The spectrum of cell death in sarcoma
Belyaeva, E. D., Loginova, N., Schroeder, B., Goldlust, I. S., Acharya, A., Kumar, S., Timashev, P. S., & Ulasov, I. V. (2023) · Biomedicine & Pharmacotherapy · doi:10.1016/j.biopha.2023.114683 · Cited by 5
Obatoclax Rescues FUS-ALS Phenotypes in iPSC-Derived Neurons by Inducing Autophagy
Bautista, C. M. C., Eismann, K., Gentzel, M., Pelucchi, S., Mertens, J., Walters, H., Yun, M. H., & Sterneckert, J. L. (2023) · Cells · doi:10.3390/cells12182247 · Cited by 4
Zinc finger domain of p62/SQSTM1 is involved in the necroptosis of human cisplatin‑resistant ovarian cancer cells treated with sulfasalazine
Liu, N., Liu, S., Zhang, X., Tian, W., Jia, H.-Q., Ye, X., Yan, X., Yu, C., & Yu, H. (2024) · Oncology Letters · doi:10.3892/ol.2024.14662 · Cited by 4
Subacute Reperfusion in Ischemic Hearts: Study of Autophagy and its Possible Interconnection with Receptor-Interacting Protein Kinase 3
Horvath, C. G., Ravíngerová, T., Suleiman, M. S., & Adameová, A. (2022) · Reviews in Cardiovascular Medicine · doi:10.31083/j.rcm2306213 · Cited by 4
The Potential Role of Necroptosis in Diseases
Linkermann, A., Berghe, T. V., Takahashi, N., Kunzendorf, U., Krautwald, S., & Vandenabeele, P. (2014) · Springer eBooks · doi:10.1007/978-1-4614-8220-8_1 · Cited by 4
Accurate elimination of superfluous attachment cells is critical for the construction of functional multicellular proprioceptors in Drosophila
Avetisyan, A. A., & Salzberg, A. (2019) · Cell Death and Differentiation · doi:10.1038/s41418-018-0260-y · Cited by 4
Glycidol induces necroptosis and inflammation through autophagy-necrosome pathway in renal cell and mice
Lu, J., Wei, H., Yao, X., Chen, Y., Liu, M., & Guan, S. (2025) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2025.178852 · Cited by 3
Loss of ADAM15 prevents necroptosis induction by partial RIPK1 degradation due to enhanced TNF-R1 surface expression and basal caspase-8 activation
Braun, S., Knackfuß, K., Ziesmann, T., Mlinzk, L., Goerg, A., Frankenheim, J., Walter, A., Schneider‐Brachert, W., Distler, U., & Fritsch, J. H. (2025) · Cell Communication and Signaling · doi:10.1186/s12964-025-02530-3 · Cited by 3
The Role of Autophagy in Cell Death
Shen, S., & Codogno, P. (2016) · Elsevier eBooks · doi:10.1016/b978-0-12-802937-4.00007-7 · Cited by 3
Cell Injury and Necrosis
d’Avila, J. C., Levin, S., & Radi, Z. A. (2017) · Elsevier eBooks · doi:10.1016/b978-0-12-801238-3.64220-4 · Cited by 3
Therapeutics targeting BCL2 family proteins
Dai, H., Meng, X. W., Ye, K., Jia, J., & Kaufmann, S. H. (2021) · Elsevier eBooks · doi:10.1016/b978-0-12-814208-0.00007-5 · Cited by 2
Mechanisms of apoptosis in ovarian cancer: The small molecule targeting
Philemon, N. U., Choene, M. S., & Motadi, L. R. (2015) · International Journal of Medicine and Medical Sciences · doi:10.5897/ijmms2014.1081 · Cited by 3
Necroptosis: A Novel Way of Regulated Necrosis with Large Pathophysiological Implications
Vandenabeele, P., Berghe, T. V., & Takahashi, N. (2014) · Elsevier eBooks · doi:10.1016/b978-0-12-386456-7.01412-x · Cited by 2
Mathematical modeling of the molecular switch of TNFR1-mediated signaling pathways using Petri netsPreprint
Amstein, L. K., Ackermann, J., Hannig, J., Đikić, I., Fulda, S., & Koch, I. (2021) · bioRxiv (Cold Spring Harbor Laboratory) · doi:10.1101/2021.11.02.466901 · Cited by 2
Self-citation
A Critical Review on BH 3 Mimetic Drugs and the Treatment of Cancer‐Associated Thrombosis ( CAT ): A Proposed Design for a Drug Delivery System Capable of Simultaneously Targeting Tumor Cells and Activated Platelets
Ghasemzadeh, M., Heidari, N., Naghinezhad, J., Ghasemzadeh, A., & Hosseini, E. (2025) · Cancer Medicine · doi:10.1002/cam4.71270 · Cited by 1
GX15-070 enhances niraparib efficacy in ovarian cancer by promoting a shift in Mcl1-mediated DNA repair pathway from HR to NHEJ
Sheng, J.-J., He, Y., Liu, P.-W., Zheng, S. L., Wu, N., Sui, H., Cheng, Q., & Li, H. (2025) · Journal of Translational Medicine · doi:10.1186/s12967-025-07284-7 · Cited by 1
Targeting autophagy and metabolism for ewing sarcoma
Antoshina, K., Aniskin, D., Stempen, M., Schroeder, B., Timashev, P. S., Ulasov, I. V., Chaudhary, A., & Kharwar, R. K. (2025) · Molecular Biology Reports · doi:10.1007/s11033-025-11304-6 · Cited by 1
Cutting edge: ESCRT-mediated phagophore closure in mammals
Takahashi, Y., Opozda, D. M., & Wang, H. (2026) · Biochemical Journal · doi:10.1042/bcj20250148 · Cited by 1
Obatoclax enhances TRAIL-induced apoptosis in non-small-cell-lung-cancer cells by increasing TRAIL receptor expression, priming for mitochondrial apoptosis and reducing XIAP levels
Sobocki, B. K., de Wilt, L. H. A. M., Snoek, B. C., Jansen, G., de Jong, S., Peters, G. J., & Kruyt, F. A. E. (2025) · Journal of Cancer Metastasis and Treatment · doi:10.20517/2394-4722.2025.07 · Cited by 1
Targeting Necroptosis in Antitumor Therapy
Chan, F. K.-M., Nailwal, H., & Moriwaki, K. (2018) · Elsevier eBooks · doi:10.1016/b978-0-12-813753-6.00013-5 · Cited by 1
Hierarchical control of coherent gene clusters defines the molecular mechanisms of glioblastoma
Tsigelny, I. F., Kouznetsova, V. L., Jiang, P., Pingle, S. C., & Kesari, S. (2015) · Molecular BioSystems · doi:10.1039/c5mb00007f
Necrostatin-1: Its Discovery and Application in Cell Death Research
Christofferson, D. E., Li, Y., & Yuan, J. (2014) · Springer eBooks · doi:10.1007/978-1-4614-8220-8_16
Keeping autophagy in cheCK1
Cheong, J. K., & Virshup, D. M. (2015) · Molecular & Cellular Oncology · doi:10.1080/23723556.2015.1045117
Fibroma Uteri in Relation to Pregnancy.
Allen, E. H. (1944) · PubMed Central
Pro-necroptotic agents in cancer therapy
Elsharkawy, A. M., & Malki, A. M. (2018) · Annals of Breast Cancer · doi:10.33582/2640-8198/1004

Authors: Basit, F., Cristofanon, S., & Fulda, S.
Affiliations: Goethe University Frankfurt (DE)
Corresponding author: Fulda, S
ORCID: Basit, F
Fulda, S
Venue: Cell Death & Differentiation
Publisher: Springer Science and Business Media LLC
Published: 2013-06-07
Volume: 20
Issue: 9
Pages: 1161-1173
ISSN: 1350-9047 (ISSN-L)
1476-5403
DOI: 10.1038/cdd.2013.45
PMC: PMC3741498
OpenAlex: W1973671033
Cited by: 211
References: 47
Citations by year: 2026: 5 · 2025: 9 · 2024: 12 · 2023: 10 · 2022: 7 · 2021: 13 · 2020: 20 · 2019: 12 · 2018: 14 · 2017: 21 · 2016: 27 · 2015: 30 · 2014: 21 · 2013: 8
FWCI: 12.85
Type: journal article
Language: en
Update notices: erratum (2014-06-09)
Open access: bronze
doi.org
Licence: www.springer.com/tdm (tdm)
www.springer.com/tdm (vor)
Locations: Cell Death and Differentiation (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Funders: Deutsche Krebshilfe
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
Life Sciences › Biochemistry, Genetics and Molecular Biology › Cancer Research › Ferroptosis and cancer prognosis
Keywords: obatoclax, necroptosis, autophagy, autophagosomes, ATG5, RIPK1, RIPK3, FADD, BCL-2 inhibitor, rhabdomyosarcoma, necrostatin-1, tumor growth inhibition, chorioallantoic membrane model, non-apoptotic cell death
MeSH terms: Autophagy-Related Protein 5, Autophagy-Related Protein 7, Autophagy, Cell Line, Humans, Imidazoles, Indoles, Microtubule-Associated Proteins, Necrosis, Phagosomes, Pyrroles, Cell Death, GTPase-Activating Proteins, RNA Interference, RNA, Small Interfering, Fas-Associated Death Domain Protein, Receptor-Interacting Protein Serine-Threonine Kinases, HEK293 Cells

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review

Obatoclax (GX15-070) triggers necroptosis by promoting the assembly of the necrosome on autophagosomal membranes

Abstract

Overview

Integrity signals

Verification

Claims the paper makes

Claims other papers make about this one

Self-check

Citations

Cites

Cited by

Related

Metadata

Record

About these checks