Genotoxic stress/p53-induced DNAJB9 inhibits the pro-apoptotic function of p53
Open accessAbstract
DNAJB9 is a recently isolated member of the molecular chaperone gene family, whose precise function is largely unknown. In the present study, we have identified DNAJB9 as an inducible gene of the tumor suppressor p53. DNAJB9 expression was induced by p53 or genotoxic stress in a p53-dependent manner, which was mediated by the Ras/Raf/ERK pathway. In addition, depletion of DNAJB9 by using siRNAs greatly increased genotoxic stress/p53-induced apoptosis, suggesting that DNAJB9 inhibits the pro-apoptotic function of p53. We also found that DNAJB9 physically interacts with p53 through its J domain, through which it inhibits the pro-apoptotic function of p53. Moreover, DNAJB9 colocalized with p53 in both cytoplasm and nucleus under genotoxic conditions. Together, these results demonstrate that DNAJB9 is a downstream target of p53 that belongs to the group of negative feedback regulators of p53.
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Quantitative proteomic analysis of protein expression changes conferred by asukamycin treatment in 231MFP cells showed many known p53 transcriptional targets upregulated, including GADD45A, GADD45B, ANKRD1, HMOX1, ATF3, PMAIP1, DDIT4, DNAJB9, and PLK2.
“quantitative proteomic analysis of protein expression changes conferred by asukamycin treatment in 231MFP cells showed many known p53 transcriptional targets upregulated in expression, including GADD45A, GADD45B, ANKRD1, HMOX1, ATF3, PMAIP1, DDIT4, DNAJB9, and PLK2 (Extended Data Figure 9) 31–37.”
From Manumycin polyketides act as molecular glues between UBR7 and P53 (2020) · cites this paper as [35]
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Cites
Vogelstein, B., Lane, D., & Levine, A. J. (2000) · Nature · doi:10.1038/35042675 · Cited by 6,607 · see citing works
Tumor suppressor p53 is a direct transcriptional activator of the human bax gene
Toshiyuki, M., & Reed, J. C. (1995) · Cell · doi:10.1016/0092-8674(95)90412-3 · Cited by 4,063 · see citing works
A simplified system for generating recombinant adenoviruses
He, T., Zhou, S., da Costa, L. T., Yu, J., Kinzler, K. W., & Vogelstein, B. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.5.2509 · Cited by 3,209 · see citing works
PUMA, a Novel Proapoptotic Gene, Is Induced by p53
Nakano, K., & Vousden, K. H. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00214-3 · Cited by 2,315 · see citing works
Noxa, a BH3-Only Member of the Bcl-2 Family and Candidate Mediator of p53-Induced Apoptosis
Oda, E., Ohki, R., Murasawa, H., Nemoto, J., Shibue, T., Yamashita, T., Tokino, T., Taniguchi, T., & Tanaka, †. N. (2000) · Science · doi:10.1126/science.288.5468.1053 · Cited by 2,112 · see citing works
Oncoprotein MDM2 is a ubiquitin ligase E3 for tumor suppressor p53
Honda, R., Tanaka, H., & Yasuda, H. (1997) · FEBS Letters · doi:10.1016/s0014-5793(97)01480-4 · Cited by 1,951 · see citing works
A novel form of Epstein-Barr virus latency in normal B cells in vivo
Miyashita, E. M., Yang, B., Lam, K. M. C., Crawford, D., & Thorley‐Lawson, D. A. (1995) · Cell · doi:10.1016/0092-8674(95)90513-8 · Cited by 1,591 · see citing works
KILLER/DR5 is a DNA damage–inducible p53–regulated death receptor gene
Wu, G. S., Burns, T. F., McDonald, E. R., Jiang, W., Meng, R., Krantz, I. D., Kao, G., Gan, D.-D., Zhou, J.-Y., Muschel, R. J., et al. (1997) · Nature Genetics · doi:10.1038/ng1097-141 · Cited by 1,081 · see citing works
The diversity of the DnaJ/Hsp40 family, the crucial partners for Hsp70 chaperones
Qiu, X., Shao, Y., Miao, S., & Wang, L. (2006) · Cellular and Molecular Life Sciences · doi:10.1007/s00018-006-6192-6 · Cited by 900 · see citing works
Induction of the growth inhibitor IGF-binding protein 3 by p53
Buckbinder, L., Talbott, R. L., Velasco-Miguel, S., Takenaka, I. M., Faha, B., Seizinger, B. R., & Kley, N. (1995) · Nature · doi:10.1038/377646a0 · Cited by 869 · see citing works
Pirh2, a p53-Induced Ubiquitin-Protein Ligase, Promotes p53 Degradation
Leng, R. P., Lin, Y., Ma, W., Wu, H., Lemmers, B., Chung, S., Parant, J. M., Lozano, G., Hakem, R., & Benchimol, S. (2003) · Cell · doi:10.1016/s0092-8674(03)00193-4 · Cited by 717 · see citing works
The ubiquitin ligase COP1 is a critical negative regulator of p53
Dornan, D., Wertz, I. E., Shimizu, H., Arnott, D. P., Frantz, G. D., Dowd, P., Rourke, K. O., Koeppen, H., & Dixit, V. M. (2004) · Nature · doi:10.1038/nature02514 · Cited by 700 · see citing works
PERP, an apoptosis-associated target of p53, is a novel member of the PMP-22/gas3 familyPreprint
Attardi, L. D., Reczek, E. E., Cosmas, C., Demicco, E. G., McCurrach, M. E., Lowe, S. W., & Jacks, T. (2000) · Genes & Development · doi:10.1101/gad.14.6.704 · Cited by 394 · see citing works
Vos, M. J., Hageman, J., Carra, S., & Kampinga, H. H. (2008) · Biochemistry · doi:10.1021/bi800639z · Cited by 391 · see citing works
Pidd, a new death-domain–containing protein, is induced by p53 and promotes apoptosis
Lin, Y., Ma, W., & Benchimol, S. (2000) · Nature Genetics · doi:10.1038/79102 · Cited by 353 · see citing works
Kaeser, M., & Iggo, R. (2001) · Proceedings of the National Academy of Sciences of the United States of America · doi:10.1073/pnas.012283399 · Cited by 346 · see citing works
Ras-dependent growth factor regulation of MEK kinase in PC12 cells
Lange, C. A., & Johnson, G. L. (1994) · Science · doi:10.1126/science.8073291 · Cited by 321 · see citing works
Wild-type p53 triggers a rapid senescence program in human tumor cells lacking functional p53
Sugrue, M. M., Shin, D. Y., Lee, S. W., & Aaronson, S. A. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.18.9648 · Cited by 290 · see citing works
Qu, L.-K., Huang, S. H., Baltzis, D., Rivas-Estilla, A.-M., Pluquet, O., Hatzoglou, M., Koumenis, C., Taya, Y., Yoshimura, A., & Koromilas, A. E. (2004) · Genes & Development · doi:10.1101/gad.1165804 · Cited by 247 · see citing works
p53‐mediated induction of Cox‐2 counteracts p53‐ or genotoxic stress‐induced apoptosis
Han, J. A., Kim, J., Ongusaha, P. P., Hwang, D. H., Ballou, L. R., Mahale, A., Aaronson, S. A., & Lee, S. W. (2002) · The EMBO Journal · doi:10.1093/emboj/cdf591 · Cited by 215 · see citing works
Self-citationShen, Y., Meunier, L., & Hendershot, L. M. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m112214200 · Cited by 215 · see citing works
Ongusaha, P. P., Kim, J., Fang, L., Wong, T. W., Yancopoulos, G. D., Aaronson, S. A., & Lee, S. W. (2003) · The EMBO Journal · doi:10.1093/emboj/cdg129 · Cited by 198 · see citing works
Self-citationThe dark side of a tumor suppressor: anti-apoptotic p53
Jänicke, R. U., Sohn, D., & Schulze‐Osthoff, K. (2008) · Cell Death and Differentiation · doi:10.1038/cdd.2008.33 · Cited by 187 · see citing works
Dong, M., Bridges, J. P., Apsley, K. S., Xu, Y. J., & Weaver, T. E. (2008) · Molecular Biology of the Cell · doi:10.1091/mbc.e07-07-0674 · Cited by 159 · see citing works
Regulation of Raf-1 and Raf-1 Mutants by Ras-Dependent and Ras-Independent Mechanisms In Vitro
Dent, P., Reardon, D. B., Morrison, D. K., et al. (1995) · Molecular and Cellular Biology · doi:10.1128/mcb.15.8.4125 · Cited by 138 · see citing works
Li, F., Li, G., Liu, G., Lee, S. W., & Aaronson, S. A. (2001) · The EMBO Journal · doi:10.1093/emboj/20.8.1931 · Cited by 132 · see citing works
Chaperone-Assisted Protein Folding in the Cell Cytoplasm
Houry, W. A. (2001) · Current Protein and Peptide Science · doi:10.2174/1389203013381134 · Cited by 127 · see citing works
Sustained activation of Ras/Raf/mitogen-activated protein kinase cascade by the tumor suppressor p53
Lee, S. W., Li, F., Igarashi, M., Ouchi, T., Lu, K. P., & Aaronson, S. A. (2000) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.150024397 · Cited by 104 · see citing works
MDG1/ERdj4, an ER‐resident DnaJ family member, suppresses cell death induced by ER stress
Kurisu, J., Honma, A., Miyajima, H., Kondo, S., Okumura, M., & Imaizumi, K. (2003) · Genes to Cells · doi:10.1046/j.1365-2443.2003.00625.x · Cited by 79 · see citing works
COX-2 regulates p53 activity and inhibits DNA damage-induced apoptosis
Choi, E.-M., Heo, J., Oh, J.-Y., et al. (2005) · Biochemical and Biophysical Research Communications · doi:10.1016/j.bbrc.2005.01.072 · Cited by 65 · see citing works
Self-citationPröls, F., Mayer, M. P., Renner, O., et al. (2001) · Experimental Cell Research · doi:10.1006/excr.2001.5294 · Cited by 39 · see citing works
Cyclooxygenase-2 functionally inactivates p53 through a physical interaction with p53
Choi, E. M., Kim, S. R., Lee, E. J., et al. (2009) · Biochimica et Biophysica Acta (BBA) - Molecular Cell Research · doi:10.1016/j.bbamcr.2009.05.006 · Cited by 28 · see citing works
High levels of the molecular chaperone Mdg1/ERdj4 reflect the activation state of endothelial cells
Berger, B. J., Müller, T. S., Buschmann, I. R., et al. (2003) · Experimental Cell Research · doi:10.1016/s0014-4827(03)00316-1 · Cited by 25 · see citing works
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Manumycin polyketides act as molecular glues between UBR7 and P53
Isobe, Y., Okumura, M., McGregor, L. M., Brittain, S. M., Jones, M. D., Liang, X., White, R., Forrester, W., McKenna, J. M., Tallarico, J. A., et al. (2020) · Nature Chemical Biology · doi:10.1038/s41589-020-0557-2 · Cited by 124 · see citing works
- Authors
- Lee, H. J., Kim, J. M., Kim, K. H., Heo, J. I., Kwak, S. J., & Han, J. A.
- Affiliations
- Kangwon National University (KR)
- Institute for Systems Biology (US)
- Hallym University (KR)
- Dankook University (KR)
- Corresponding author
- Han, J A
- Venue
- Cell Death & Differentiation
- Published
- 2014-08-22
- Volume
- 22
- Issue
- 1
- Pages
- 86-95
- ISSN
- 1350-9047 (ISSN-L)
- 1476-5403
- PMC
- PMC4262772
- OpenAlex
- W2006429697
- Cited by
- 60
- References
- 33
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 4 · 2023: 4 · 2022: 3 · 2021: 13 · 2020: 10 · 2019: 6 · 2017: 5 · 2016: 5 · 2015: 2
- FWCI
- 1.93
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Death and Differentiation (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Research Foundation
- National Research Foundation of Korea
- Ministry of Education, Science and Technology
- Topics
- Health Sciences › Medicine › Oncology › Cancer-related Molecular Pathways
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Heat shock proteins research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
- Keywords
- DNAJB9, p53, genotoxic stress, apoptosis, molecular chaperones, Ras/Raf/ERK pathway, p53 target genes, negative feedback regulation, J-domain, p53 stabilization, tumor suppressor
- MeSH terms
- Animals, Cell Nucleus, Cytoplasm, DNA Damage, Humans, Membrane Proteins, Protein Binding, Tumor Suppressor Protein p53, Apoptosis, Protein Structure, Tertiary, Mice, Knockout, Molecular Chaperones, MAP Kinase Signaling System, Cell Line, Tumor, HSP40 Heat-Shock Proteins, Mice
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