Efficient generation of hepatocyte-like cells from human induced pluripotent stem cells
Abstract
Human induced pluripotent stem (iPS) cells are similar to embryonic stem (ES) cells, and can proliferate intensively and differentiate into a variety of cell types. However, the hepatic differentiation of human iPS cells has not yet been reported. In this report, human iPS cells were induced to differentiate into hepatic cells by a stepwise protocol. The expression of liver cell markers and liver-related functions of the human iPS cell-derived cells were monitored and compared with that of differentiated human ES cells and primary human hepatocytes. Approximately 60% of the differentiated human iPS cells at day 7 expressed hepatic markers alpha fetoprotein and Alb. The differentiated cells at day 21 exhibited liver cell functions including albumin Asecretion, glycogen synthesis, urea production and inducible cytochrome P450 activity. The expression of hepatic markers and liver-related functions of the iPS cell-derived hepatic cells were comparable to that of the human ES cell-derived hepatic cells. These results show that human iPS cells, which are similar to human ES cells, can be efficiently induced to differentiate into hepatocyte-like cells.
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HLCs have lower hepatic function than PHHs and almost no proliferative capacity.
“However, HLCs have lower hepatic function than PHHs and almost no proliferative capacity. 13)”
From Cryopreservation of Hepatocyte Organoid-Derived Cells Generated from Human iPS Cells (2025) · cites this paper as [13]
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Cryopreservation of Hepatocyte Organoid-Derived Cells Generated from Human iPS Cells
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- Authors
- Song, Z., Cai, J., Liu, Y., Zhao, D., Yong, J., Duo, S., Song, X., Guo, Y., Zhao, Y., Qin, H., Yin, X., Wu, C., Che, J., Lu, S., Ding, M., & Deng, H.
- Affiliations
- Peking University (CN)
- Capital Medical University (CN)
- Corresponding author
- Deng, Hongkui
- ORCID
- Venue
- Cell Research
- Published
- 2009-09-08
- Volume
- 19
- Issue
- 11
- Pages
- 1233-1242
- ISSN
- 1001-0602 (ISSN-L)
- 1748-7838
- OpenAlex
- W1985842788
- Cited by
- 499
- References
- 2
- Citations by year
- 2026: 9 · 2025: 13 · 2024: 10 · 2023: 16 · 2022: 19 · 2021: 20 · 2020: 29 · 2019: 21 · 2018: 39 · 2017: 28 · 2016: 30 · 2015: 42 · 2014: 38 · 2013: 43 · 2012: 44
- FWCI
- 18.77
- Type
- journal article
- Language
- en
- Open access
- closed
- Licence
- www.springer.com/tdm (tdm)
- Locations
- Cell Research (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
- Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
- Health Sciences › Medicine › Surgery › Pancreatic function and diabetes
- Keywords
- induced pluripotent stem cells, hepatocyte-like cells, hepatic differentiation, alpha-fetoprotein, albumin secretion, glycogen synthesis, urea synthesis, cytochrome P450, embryonic stem cells, primary hepatocytes
- MeSH terms
- Serum Albumin, Human, alpha-Fetoproteins, Cell Differentiation, Cell Line, Cells, Cultured, Cytochrome P-450 Enzyme System, Glycoproteins, Humans, Liver Glycogen, Serum Albumin, Urea, Hepatocytes, Embryonic Stem Cells, Induced Pluripotent Stem Cells
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