The DOI matches its Crossref record; 47 of the 47 references it cites were found in a registry or an index.
Mechanism and functions of membrane binding by the Atg5–Atg12/Atg16 complex during autophagosome formation
Open accessAbstract
Autophagy is a conserved process for the bulk degradation of cytoplasmic material. Triggering of autophagy results in the formation of double membrane‐bound vesicles termed autophagosomes. The conserved Atg5–Atg12/Atg16 complex is essential for autophagosome formation. Here, we show that the yeast Atg5–Atg12/Atg16 complex directly binds membranes. Membrane binding is mediated by Atg5, inhibited by Atg12 and activated by Atg16. In a fully reconstituted system using giant unilamellar vesicles and recombinant proteins, we reveal that all components of the complex are required for efficient promotion of Atg8 conjugation to phosphatidylethanolamine and are able to assign precise functions to all of its components during this process. In addition, we report that in vitro the Atg5–Atg12/Atg16 complex is able to tether membranes independently of Atg8. Furthermore, we show that membrane binding by Atg5 is downstream of its recruitment to the pre‐autophagosomal structure but is essential for autophagy and cytoplasm‐to‐vacuole transport at a stage preceding Atg8 conjugation and vesicle closure. Our findings provide important insights into the mechanism of action of the Atg5–Atg12/Atg16 complex during autophagosome formation.
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Cites
Repertoires of Autophagy in the Pathogenesis of Ocular Diseases
Li, Y.-J., Jiang, Q., Cao, G.-F., Yao, J., & Yan, B. (2015) · Cellular Physiology and Biochemistry · doi:10.1159/000373980 · Cited by 16,304
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Moy, R. H., & Cherry, S. (2013) · Journal of Innate Immunity · doi:10.1159/000350326 · Cited by 3,539
Autophagosome formation: core machinery and adaptations
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A ubiquitin-like system mediates protein lipidation
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Dynamics and diversity in autophagy mechanisms: lessons from yeast
Nakatogawa, H., Suzuki, K., Kamada, Y., & Ohsumi, Y. (2009) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2708 · Cited by 1,725
A protein conjugation system essential for autophagy
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Mitochondria Supply Membranes for Autophagosome Biogenesis during Starvation
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Dissection of Autophagosome Formation Using Apg5-Deficient Mouse Embryonic Stem Cells
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Dan Dunn, J., Alvarez, L. A., Zhang, X., & Soldati, T. (2015) · Redox Biology · doi:10.1016/j.redox.2015.09.005 · Cited by 1,203
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Gao, W., Wang, X., Zhou, Y., Wang, X., & Yu, Y. (2022) · Signal Transduction and Targeted Therapy · doi:10.1038/s41392-022-01046-3 · Cited by 1,042
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Li, Y.-J., Lei, Y.-H., Yao, N., Wang, C.-R., Hu, N., Ye, W.-C., Zhang, D.-M., & Chen, Z.-S. (2017) · Chinese Journal of Cancer · doi:10.1186/s40880-017-0219-2 · Cited by 665
Metadata
- Authors
- Romanov, J., Walczak, M., Ibiricu, I., Schüchner, S., Ogris, E., Kraft, C., & Martens, S.
- Venue
- The EMBO Journal
- Published
- 2012-10-12
- Volume
- 31
- Issue
- 22
- Pages
- 4304-4317
- ISSN
- 0261-4189 (ISSN-L)
- 1460-2075
- OpenAlex
- W2149572123
- Cited by
- 486
- References
- 47
- FWCI
- 17.66
- Type
- journal article
- Language
- en
- Open access
- green
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- Romanov, J., Walczak, M., Ibiricu, I., Schüchner, S., Ogris, E., Kraft, C., & Martens, S. (2012). Mechanism and functions of membrane binding by the Atg5–Atg12/Atg16 complex during autophagosome formation. The EMBO Journal. https://doi.org/10.1038/emboj.2012.278
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