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Mitochondria Supply Membranes for Autophagosome Biogenesis during Starvation

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Hailey, Dale W., Rambold, Angelika S., Satpute-Krishnan, Prasanna, Mitra, Kasturi, Sougrat, Rachid, Kim, Peter K., Lippincott-Schwartz, Jennifer·2010·Cell
DOI 10.1016/j.cell.2010.04.009
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Abstract

Starvation-induced autophagosomes engulf cytosol and/or organelles and deliver them to lysosomes for degradation, thereby re-supplying depleted nutrients. Despite advances in understanding the molecular basis of this process, the membrane origin of autophagosomes remains unclear. Here, we demonstrate that, in starved cells, autophagosomes are derived from the outer membranes of mitochondria. In time-lapse movies, the early autophagosomal marker, mApg5, transiently localizes to punctae on the surface of mitochondria, followed by the late autophagosomal marker, LC3. A unique tail-anchored outer mitochondrial membrane protein, but not other outer nor inner mitochondrial membrane proteins, labels autophagosomes and diffuses into newly forming autophagosomes from mitochondria. The fluorescent lipid, NBD-PS (which converts to PE in mitochondria) transfers from mitochondria to autophagosomes in starved cells. In addition, when mitochondria/ER connections are perturbed by loss of mitofusin2, starvation-induced autophagosomes do not form. Mitochondria thus play a central role in starvation-induced autophagy, serving as membrane source of autophagosomes.

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Metadata

Authors
Hailey, D. W., Rambold, A. S., Satpute-Krishnan, P., Mitra, K., Sougrat, R., Kim, P. K., & Lippincott-Schwartz, J.
Venue
Cell
Publisher
Elsevier BV
Published
2010-05
Volume
141
Issue
4
Pages
656-667
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/j.cell.2010.04.009
OpenAlex
W2132763961
Cited by
1,429
References
9
FWCI
88.69
Type
journal article
Language
en
Open access
bronze
Licence

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CoChat. (2026). Citation check: Mitochondria Supply Membranes for Autophagosome Biogenesis during Starvation. https://app.cochat.ai/papers/10.1016/j.cell.2010.04.009
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Hailey, D. W., Rambold, A. S., Satpute-Krishnan, P., Mitra, K., Sougrat, R., Kim, P. K., & Lippincott-Schwartz, J. (2010). Mitochondria Supply Membranes for Autophagosome Biogenesis during Starvation. Cell. https://doi.org/10.1016/j.cell.2010.04.009

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