The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Native human adipose stromal cells: localization, morphology and phenotype
Open accessAbstract
OBJECTIVES: Beside having roles in energy homeostasis and endocrine modulation, adipose tissue (AT) is now considered a promising source of mesenchymal stromal cells (adipose-derived stromal cells or ASCs) for regenerative medicine. Despite numerous studies on cultured ASCs, native human ASCs are rarely investigated. Indeed, the phenotype of ASCs in their native state, their localization within AT and comparison with bone marrow-derived mesenchymal stromal cells (BM-MSCs) has been poorly investigated. DESIGN: To address these issues, the stroma vascular fraction (SVF) of human AT was extracted and native cell subtypes were isolated by immunoselection to study their clonogenic potential in culture. Immunohistology on samples of human AT in combination with reconstruction of confocal sections were performed in order to localize ASCs. RESULTS: Compared with BM-MNCs, all native ASCs were found in the CD34(+) cell fraction of the AT-SVF. Native ASCs expressed classical mesenchymal markers described for BM-MSCs. Interestingly, CD34 expression decreased during ASC cell culture and was negatively correlated with cell proliferation rate. Immunohistological analysis revealed that native ASCs exhibited specific morphological features with protrusions. They were found scattered in AT stroma and did not express in vivo pericytic markers such as NG2, CD140b or alpha-smooth muscle actin, which appeared during the culture process. Finally, ASCs spontaneous commitment to adipocytic lineage was enhanced in AT from obese humans. CONCLUSIONS: The use of complementary methodological approaches to study native human ASCs revealed their immunophenotype, their specific morphology, their location within AT and their stemness. Furthermore, our data strongly suggest that human ASCs participate in adipogenesis during AT development.
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Cited by
Bourin, P., Bunnell, B. A., Casteilla, L., Dominici, M., Katz, A. J., March, K. L., Redl, H., Rubin, J. P., Yoshimura, K., & Gimble, J. M. (2013) · Cytotherapy · doi:10.1016/j.jcyt.2013.02.006 · Cited by 1,890
“CD13þ and CD73þ cells have been described to represent around 80% and 70% of the CD45 CD31 CD34þ cell population, respectively (26).”
BackgroundResultSelf-citationMazini, L., Rochette, L., Amine, M., & Malka, G. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20102523 · Cited by 445
The Life and Fate of Mesenchymal Stem Cells
Eggenhofer, E., Luk, F., Dahlke, M. H., & Hoogduijn, M. J. (2014) · Frontiers in Immunology · doi:10.3389/fimmu.2014.00148 · Cited by 444
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Is CD34 truly a negative marker for mesenchymal stromal cells?
Lin, C.-S., Ning, H., Lin, G., & Lue, T. F. (2012) · Cytotherapy · doi:10.3109/14653249.2012.729817 · Cited by 239
Metadata
- Authors
- Maumus, M., Peyrafitte, J.-A., D'Angelo, R., Fournier-Wirth, C., Bouloumié, A., Casteilla, L., Sengenès, C., & Bourin, P.
- Venue
- International Journal of Obesity
- Published
- 2011-01-25
- Volume
- 35
- Issue
- 9
- Pages
- 1141-1153
- ISSN
- 0307-0565 (ISSN-L)
- 1476-5497
- OpenAlex
- W1971881510
- Cited by
- 262
- References
- 12
- FWCI
- 12.42
- Type
- journal article
- Language
- en
- Open access
- hybrid
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