Cited by 3,991Mesenchymal stem cell researchHematopoietic Stem Cell TransplantationPluripotent Stem Cells Research

A perivascular origin for mesenchymal stem cells in multiple human organs

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Crisan, Mihaela, Yap, Solomon, Casteilla, Louis, Chen, Chien-Wen, Corselli, Mirko, Park, Tea Soon, Andriolo, Gabriella, Sun, Bin +13 more·2008·Cell Stem Cell

Abstract

Mesenchymal stem cells (MSCs), the archetypal multipotent progenitor cells derived in cultures of developed organs, are of unknown identity and native distribution. We have prospectively identified perivascular cells, principally pericytes, in multiple human organs including skeletal muscle, pancreas, adipose tissue, and placenta, on CD146, NG2, and PDGF-Rbeta expression and absence of hematopoietic, endothelial, and myogenic cell markers. Perivascular cells purified from skeletal muscle or nonmuscle tissues were myogenic in culture and in vivo. Irrespective of their tissue origin, long-term cultured perivascular cells retained myogenicity; exhibited at the clonal level osteogenic, chondrogenic, and adipogenic potentials; expressed MSC markers; and migrated in a culture model of chemotaxis. Expression of MSC markers was also detected at the surface of native, noncultured perivascular cells. Thus, blood vessel walls harbor a reserve of progenitor cells that may be integral to the origin of the elusive MSCs and other related adult stem cells.

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Crossref · 10.1016/j.stem.2008.07.003

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  • MSCs can function as perivascular-like support cells, physically associating with endothelial networks and promoting their architecture through direct contact mechanisms.

    “MSCs can function as perivascular-like support cells, physically associating with endothelial networks and promoting their architecture through direct contact mechanisms (Au et al., 2008; Crisan et al., 2008).”

    From APOE3-Christchurch variant enhances neurovascular support functions of iPSC-derived mesenchymal stromal cells (2026) · cites this paper as Crisan et al., 2008

    The Discussion (p. 10) links MSCs to perivascular cells, and the vessel-staining description (p. 3) shows those cells surrounding endothelial cells. The paper does not show that MSCs promote endothelial-network architecture through direct contact. (LLM, conf=0.88)

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Authors
Crisan, M., Yap, S., Casteilla, L., Chen, C.-W., Corselli, M., Park, T. S., Andriolo, G., Sun, B., Zheng, B., Zhang, L., Norotte, C., Teng, P.-N., Traas, J., Schugar, R., Deasy, B. M., Badylak, S., Bűhring, H.-J., Giacobino, J.-P., Lazzari, L., Huard, J., & Péault, B.
Affiliations
  • University of Pittsburgh (US)
  • Centre National de la Recherche Scientifique (FR)
  • Fondazione IRCCS Ca' Granda Ospedale Maggiore Policlinico (IT)
  • McGowan Institute for Regenerative Medicine (US)
  • Children's Hospital of Philadelphia (US)
Corresponding author
Péault, Bruno
Venue
Cell Stem Cell
Publisher
Elsevier BV
Published
2008-09
Volume
3
Issue
3
Pages
301-313
ISSN
  • 1875-9777 (ISSN-L)
  • 1934-5909
OpenAlex
W2076491580
Cited by
3,991
References
47
Citations by year
2026: 77 · 2025: 92 · 2024: 130 · 2023: 175 · 2022: 173 · 2021: 247 · 2020: 240 · 2019: 247 · 2018: 271 · 2017: 259 · 2016: 291 · 2015: 296 · 2014: 281 · 2013: 305 · 2012: 301
FWCI
91.59
Type
journal article
Language
en
Open access
Locations
  • Cell stem cell (publishedVersion)
  • PubMed (publishedVersion)
  • D-Scholarship@Pitt (University of Pittsburgh) (acceptedVersion)
  • Edinburgh Research Explorer (University of Edinburgh) (submittedVersion, other-oa)
Funders
  • Medical Center, University of Pittsburgh
  • National Heart, Lung, and Blood Institute (R21HL083057)
  • National Institute of Arthritis and Musculoskeletal and Skin Diseases (R01AR049684, R01 AR49684-01)
Topics
  • Health Sciences › Medicine › Genetics › Mesenchymal stem cell research
  • Health Sciences › Medicine › Hematology › Hematopoietic Stem Cell Transplantation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Pluripotent Stem Cells Research
Keywords
mesenchymal stem cells, perivascular cells, pericytes, stem cell origin, CD146, NG2, osteogenic differentiation, chondrogenic differentiation, adipogenesis, chemotaxis, skeletal muscle, pancreas, adipose tissue, placenta, adult stem cells, vascular wall
MeSH terms
Adolescent, Adult, Aged, Cell Movement, Fetus, Flow Cytometry, Humans, Middle Aged, Pericytes, Muscle Development, Fetal Stem Cells, Adult Stem Cells, Mesenchymal Stem Cells

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