The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Autotrophic growth of nitrifying community in an agricultural soil
Open accessAbstract
The two-step nitrification process is an integral part of the global nitrogen cycle, and it is accomplished by distinctly different nitrifiers. By combining DNA-based stable isotope probing (SIP) and high-throughput pyrosequencing, we present the molecular evidence for autotrophic growth of ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and nitrite-oxidizing bacteria (NOB) in agricultural soil upon ammonium fertilization. Time-course incubation of SIP microcosms indicated that the amoA genes of AOB was increasingly labeled by 13CO2 after incubation for 3, 7 and 28 days during active nitrification, whereas labeling of the AOA amoA gene was detected to a much lesser extent only after a 28-day incubation. Phylogenetic analysis of the 13C-labeled amoA and 16S rRNA genes revealed that the Nitrosospira cluster 3-like sequences dominate the active AOB community and that active AOA is affiliated with the moderately thermophilic Nitrososphaera gargensis from a hot spring. The higher relative frequency of Nitrospira-like NOB in the 13C-labeled DNA suggests that it may be more actively involved in nitrite oxidation than Nitrobacter-like NOB. Furthermore, the acetylene inhibition technique showed that 13CO2 assimilation by AOB, AOA and NOB occurs only when ammonia oxidation is not blocked, which provides strong hints for the chemolithoautotrophy of nitrifying community in complex soil environments. These results show that the microbial community of AOB and NOB dominates the nitrification process in the agricultural soil tested.
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Cited by
Nitrification and nitrifiers in acidic soils
Li, Y., Chapman, S. J., Nicol, G. W., & Yao, H. (2018) · Soil Biology and Biochemistry · doi:10.1016/j.soilbio.2017.10.023 · Cited by 523
Controls and Adaptive Management of Nitrification in Agricultural Soils
Norton, J., & Ouyang, Y. (2019) · Frontiers in Microbiology · doi:10.3389/fmicb.2019.01931 · Cited by 404
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Self-citationSynthetic microbial consortia for biosynthesis and biodegradation: promises and challenges
Che, S., & Men, Y. (2019) · Journal of Industrial Microbiology and Biotechnology · doi:10.1007/s10295-019-02211-4 · Cited by 177
Cai, M., Ng, S.-K., Lim, C. K., Lu, H., Jia, Y., & Lee, P. K. H. (2018) · Frontiers in Microbiology · doi:10.3389/fmicb.2018.00280 · Cited by 25
Metadata
- Authors
- Xia, W., Zhang, C., Zeng, X., Feng, Y., Weng, J., Lin, X., Zhu, J., Xiong, Z., Xu, J., Cai, Z., & Jia, Z.
- Venue
- The ISME Journal
- Publisher
- Oxford University Press (OUP)
- Published
- 2011-02-17
- Volume
- 5
- Issue
- 7
- Pages
- 1226-1236
- ISSN
- 1751-7362 (ISSN-L)
- 1751-7370
- OpenAlex
- W2064012286
- Cited by
- 401
- References
- 61
- FWCI
- 42.60
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Xia, W., Zhang, C., Zeng, X., Feng, Y., Weng, J., Lin, X., Zhu, J., Xiong, Z., Xu, J., Cai, Z., & Jia, Z. (2011). Autotrophic growth of nitrifying community in an agricultural soil. The ISME Journal. https://doi.org/10.1038/ismej.2011.5
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