The DOI matches its Crossref record; 32 of the 33 references it cites were found in a registry or an index.
Evaluation of the PBR/TSPO Radioligand [ 18 F]DPA-714 in a Rat Model of Focal Cerebral Ischemia
Open accessAbstract
Focal cerebral ischemia leads to an inflammatory reaction involving an overexpression of the peripheral benzodiazepine receptor (PBR)/18-kDa translocator protein (TSPO) in the cerebral monocytic lineage (microglia and monocyte) and in astrocytes. Imaging of PBR/TSPO by positron emission tomography (PET) using radiolabeled ligands can document inflammatory processes induced by cerebral ischemia. We performed in vivo PET imaging with [(18)F]DPA-714 to determine the time course of PBR/TSPO expression over several days after induction of cerebral ischemia in rats. In vivo PET imaging showed significant increase in DPA (N,N-diethyl-2-(2-(4-(2-fluoroethoxy)phenyl)-5,7-dimethylpyrazolo[1,5-a]pyrimidin-3-yl)acetamide) uptake on the injured side compared with that in the contralateral area on days 7, 11, 15, and 21 after ischemia; the maximal binding value was reached 11 days after ischemia. In vitro autoradiography confirmed these in vivo results. In vivo and in vitro [(18)F]DPA-714 binding was displaced from the lesion by PK11195 and DPA-714. Immunohistochemistry showed increased PBR/TSPO expression, peaking at day 11 in cells expressing microglia/macrophage antigens in the ischemic area. At later times, a centripetal migration of astrocytes toward the lesion was observed, promoting the formation of an astrocytic scar. These results show that [(18)F]DPA-714 provides accurate quantitative information of the time course of PBR/TSPO expression in experimental stroke.
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“Similar to previous studies, TSPO expression was found in microglia in active and chronic active lesions (Cosenza-Nashat et al., 2009; Martin et al., 2010; Lavisse et al., 2012a; Loth et al., 2016; Kaunzner et al., 2019).”
From A quantitative neuropathological assessment of translocator protein expression in multiple sclerosis (2019) · cites this paper as Martin et al., 2010
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Cites
Microglia: a sensor for pathological events in the CNS
Kreutzberg, G. W. (1996) · Trends in Neurosciences · doi:10.1016/0166-2236(96)10049-7 · Cited by 4,396
Simplified Reference Tissue Model for PET Receptor Studies
Lammertsma, A. A., & Hume, S. P. (1996) · NeuroImage · doi:10.1006/nimg.1996.0066 · Cited by 2,025
Papadopoulos, V., Baraldi, M., Guilarte, T. R., Knudsen, T. B., Lacapère, J.-J., Lindemann, P., Norenberg, M. D., Nutt, D., Weizman, A., Zhang, M.-R., et al. (2006) · Trends in Pharmacological Sciences · doi:10.1016/j.tips.2006.06.005 · Cited by 1,389
In-vivo measurement of activated microglia in dementia
Cagnin, A., Brooks, D. J., Kennedy, A. M., Gunn, R. N., Myers, R., Turkheimer, F. E., Jones, T., & Banati, R. B. (2001) · The Lancet · doi:10.1016/s0140-6736(01)05625-2 · Cited by 1,054
The peripheral benzodiazepine binding site in the brain in multiple sclerosis
Banati, R. B., Newcombe, J., Gunn, R. N., Cagnin, A., Turkheimer, F., Heppner, F., Price, G., Wegner, F., Giovannoni, G., Miller, D. H., et al. (2000) · Brain · doi:10.1093/brain/123.11.2321 · Cited by 661
Translocator protein 18 kDa (TSPO): Molecular sensor of brain injury and repair
Chen, M.-K., & Guilarte, T. R. (2008) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2007.12.004 · Cited by 506
Belayev, L., Busto, R., Zhao, W., & Ginsberg, M. D. (1996) · Brain Research · doi:10.1016/s0006-8993(96)00815-3 · Cited by 505
Nuclear imaging of neuroinflammation: a comprehensive review of [11C]PK11195 challengers
Chauveau, F., Boutin, H., Camp, N. V., Dollé, F., & Tavitian, B. (2008) · European Journal of Nuclear Medicine and Molecular Imaging · doi:10.1007/s00259-008-0908-9 · Cited by 419
Self-citationEvolution of microglial activation in patients after ischemic stroke: a [11C](R)-PK11195 PET study
Gerhard, A., Schwarz, J., Myers, R., Wise, R. J. S., & Banati, R. B. (2004) · NeuroImage · doi:10.1016/j.neuroimage.2004.09.034 · Cited by 286
James, M. L., Fulton, R., Vercouillie, J., Henderson, D., Garreau, L., Chalon, S., Dollé, F., Selleri, S., Guilloteau, D., & Kassiou, M. (2008) · Journal of Nuclear Medicine · doi:10.2967/jnumed.107.046151 · Cited by 283
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1 of the 33 references couldn’t be matched to a known paper.
Research works printed but not linked: 3
- Chen MK, 2008, Pharmacol Ther, 2008, 118
- Lammertsma AA, 1996, Neuroimage, 19, 312
- Le Pogam A, 2008, J Nucl Med, 49, 231P
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Al-Khishman, N. U., Qi, Q., Roseborough, A. D., Levit, A., Allman, B. L., Anazodo, U., Fox, M. S., Whitehead, S. N., & Thiessen, J. D. (2020) · Figshare · doi:10.6084/m9.figshare.13023330
PET molecular imaging of peripheral and central inflammatory processes targeting the TSPO 18 kDa
Bernards, N. (2014) · theses.fr (ABES) · doi:10.70675/0fcb5c23z4908z43acz82a6z4b5a0c4a1471
“TSPO increase in microglia is now presented as a hallmark of brain inflammation and our laboratories have been instrumental in the characterization and validation of this radiotracer in various preclinical models of neuroinflammation [14, 15].”
BindingDB Entry 50011999: Reversing binding sensitivity to A147T translocator protein.
BindingDB (2022) · UC San Diego · doi:10.7270/q2fj2mf6
Metadata
- Authors
- Martín, A., Boisgard, R., Thézé, B., Van Camp, N., Kuhnast, B., Damont, A., Kassiou, M., Dollé, F., & Tavitian, B.
- Affiliations
- Inserm (FR)
- INSERM U803, Orsay, France
- CEA, DSV, FBM, SHFJ, Laboratoire Imagerie Moléculaire Expérimentale, Orsay, France
- Commissariat à l'Énergie Atomique et aux Énergies Alternatives (FR)
- The University of Sydney (AU)
- Corresponding author
- Tavitian, Bertrand
- Venue
- Journal of Cerebral Blood Flow & Metabolism
- Publisher
- SAGE Publications
- Published
- 2009-09-30
- Volume
- 30
- Issue
- 1
- Pages
- 230-241
- ISSN
- 0271-678X (ISSN-L)
- 1559-7016
- PMC
- PMC2949091
- OpenAlex
- W1982808247
- Cited by
- 206
- References
- 33
- Citations by year
- 2025: 6 · 2024: 6 · 2023: 6 · 2022: 7 · 2021: 39 · 2020: 23 · 2019: 11 · 2018: 11 · 2017: 8 · 2016: 12 · 2015: 15 · 2014: 14 · 2013: 11 · 2012: 12
- FWCI
- 7.48
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Cerebral Blood Flow & Metabolism (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Neuroscience › Neurology › Neuroinflammation and Neurodegeneration Mechanisms
- Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Pharmacology › Pharmacological Receptor Mechanisms and Effects
- Health Sciences › Medicine › Radiology, Nuclear Medicine and Imaging › Medical Imaging Techniques and Applications
- Keywords
- [18F]DPA-714, focal cerebral ischemia, stroke, neuroinflammation, microglia/macrophages, astrocytes, PET radiotracer, rat model
- MeSH terms
- Animals, Astrocytes, Autoradiography, Carrier Proteins, Cell Count, Brain Ischemia, Data Interpretation, Statistical, Fluorine Radioisotopes, Image Processing, Computer-Assisted, Immunohistochemistry, Inflammation, Male, Pyrazoles, Pyrimidines, Receptors, GABA-A, Models, Statistical, Rats, Sprague-Dawley, Microglia, Radiopharmaceuticals, Stroke, Positron-Emission Tomography, Rats
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