Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks
Open accessAbstract
A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol and efficient single cell dissociation to yield more than 500,000 podocytes per mouse allowing for global, unbiased downstream applications. Combining mRNA and miRNA transcriptional profiling with quantitative proteomic analyses revealed programs of highly specific gene regulation tightly controlling cytoskeleton, cell differentiation, endosomal transport, and peroxisome function in podocytes. Strikingly, the analyses further predict that these podocyte-specific gene regulatory networks are accompanied by alternative splicing of respective genes. Thus, our 'omics' approach will facilitate the discovery and integration of novel gene, protein, and organelle regulatory networks that deepen our systematic understanding of podocyte biology.
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Single-cell podocyte sorting previously enabled only incomplete in vivo analysis of the podocyte proteome.
“Single-cell podocyte sorting previously enabled only incomplete in vivo analysis of the podocyte proteome”
From A Multi-layered Quantitative In Vivo Expression Atlas of the Podocyte Unravels Kidney Disease Candidate Genes (2018) · cites this paper as Boerries et al., 2013
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- Authors
- Boerries, M., Grahammer, F., Eiselein, S., Buck, M., Meyer, C., Goedel, M., Bechtel, W., Zschiedrich, S., Pfeifer, D., Laloë, D., Arrondel, C., Gonçalves, S., Krüger, M., Harvey, S. J., Busch, H., Dengjel, J., & Huber, T. B.
- Affiliations
- University of Freiburg (DE)
- University Medical Center Freiburg (DE)
- Hôpital Necker-Enfants Malades (FR)
- Max Planck Institute for Heart and Lung Research (DE)
- Corresponding author
- Grahammer, Florian
- Busch, Hauke
- Huber, Tobias B.
- ORCID
- Venue
- Kidney International
- Publisher
- Elsevier BV
- Published
- 2013-06
- Volume
- 83
- Issue
- 6
- Pages
- 1052-1064
- ISSN
- 0085-2538 (ISSN-L)
- OpenAlex
- W2040670636
- Cited by
- 141
- References
- 44
- Citations by year
- 2026: 2 · 2025: 4 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 14 · 2020: 14 · 2019: 6 · 2018: 9 · 2017: 16 · 2016: 20 · 2015: 16 · 2014: 16 · 2013: 6
- FWCI
- 14.56
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- Locations
- Kidney International (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Funders
- Deutsche Forschungsgemeinschaft (KFO201, (EXC 294))
- Institut National de la Santé et de la Recherche Médicale
- Bundesministerium für Bildung und Forschung (EXC 294)
- Topics
- Health Sciences › Medicine › Nephrology › Renal Diseases and Glomerulopathies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
- Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
- Keywords
- podocytes, gene regulatory networks, miRNA expression profiling, alternative splicing, cytoskeleton, cell differentiation, endosomal transport, peroxisome function, single-cell dissociation
- MeSH terms
- Animals, Cell Separation, Genotype, Luminescent Proteins, Mice, Transgenic, Phenotype, Mass Spectrometry, Signal Transduction, Alternative Splicing, Genes, Reporter, Computational Biology, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Proteomics, Podocytes, Mice, Gene Regulatory Networks
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