Cited by 141Renal Diseases and GlomerulopathiesRenal and related cancersChronic Kidney Disease and Diabetes

Molecular fingerprinting of the podocyte reveals novel gene and protein regulatory networks

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Boerries, Melanie, Grahammer, Florian, Eiselein, Sven, Buck, Moritz, Meyer, Charlotte, Goedel, Markus, Bechtel, Wibke, Zschiedrich, Stefan +9 more·2013·Kidney International

Abstract

A thorough characterization of the transcriptome and proteome of endogenous podocytes has been hampered by low cell yields during isolation. Here we describe a double fluorescent reporter mouse model combined with an optimized bead perfusion protocol and efficient single cell dissociation to yield more than 500,000 podocytes per mouse allowing for global, unbiased downstream applications. Combining mRNA and miRNA transcriptional profiling with quantitative proteomic analyses revealed programs of highly specific gene regulation tightly controlling cytoskeleton, cell differentiation, endosomal transport, and peroxisome function in podocytes. Strikingly, the analyses further predict that these podocyte-specific gene regulatory networks are accompanied by alternative splicing of respective genes. Thus, our 'omics' approach will facilitate the discovery and integration of novel gene, protein, and organelle regulatory networks that deepen our systematic understanding of podocyte biology.

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Authors
Boerries, M., Grahammer, F., Eiselein, S., Buck, M., Meyer, C., Goedel, M., Bechtel, W., Zschiedrich, S., Pfeifer, D., Laloë, D., Arrondel, C., Gonçalves, S., Krüger, M., Harvey, S. J., Busch, H., Dengjel, J., & Huber, T. B.
Affiliations
  • University of Freiburg (DE)
  • University Medical Center Freiburg (DE)
  • Hôpital Necker-Enfants Malades (FR)
  • Max Planck Institute for Heart and Lung Research (DE)
Corresponding author
  • Grahammer, Florian
  • Busch, Hauke
  • Huber, Tobias B.
Venue
Kidney International
Publisher
Elsevier BV
Published
2013-06
Volume
83
Issue
6
Pages
1052-1064
ISSN
0085-2538 (ISSN-L)
OpenAlex
W2040670636
Cited by
141
References
44
Citations by year
2026: 2 · 2025: 4 · 2024: 5 · 2023: 5 · 2022: 8 · 2021: 14 · 2020: 14 · 2019: 6 · 2018: 9 · 2017: 16 · 2016: 20 · 2015: 16 · 2014: 16 · 2013: 6
FWCI
14.56
Type
journal article
Language
en
Open access
Locations
  • Kidney International (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Funders
  • Deutsche Forschungsgemeinschaft (KFO201, (EXC 294))
  • Institut National de la Santé et de la Recherche Médicale
  • Bundesministerium für Bildung und Forschung (EXC 294)
Topics
  • Health Sciences › Medicine › Nephrology › Renal Diseases and Glomerulopathies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Renal and related cancers
  • Health Sciences › Medicine › Nephrology › Chronic Kidney Disease and Diabetes
Keywords
podocytes, gene regulatory networks, miRNA expression profiling, alternative splicing, cytoskeleton, cell differentiation, endosomal transport, peroxisome function, single-cell dissociation
MeSH terms
Animals, Cell Separation, Genotype, Luminescent Proteins, Mice, Transgenic, Phenotype, Mass Spectrometry, Signal Transduction, Alternative Splicing, Genes, Reporter, Computational Biology, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, MicroRNAs, Proteomics, Podocytes, Mice, Gene Regulatory Networks

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