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Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane

Chow, Amy, Toomre, Derek, Garrett, Wendy, Mellman, Ira·2002·Nature
DOI 10.1038/nature01006
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Abstract

Central to the initiation of immune responses is recognition of peptide antigen by T lymphocytes. The cell biology of dendritic cells makes them ideally suited for the essential process of antigen presentation. Their life cycle includes several stages characterized by distinct functions and mechanisms of regulation. Immature dendritic cells synthesize large amounts of major histocompatibility complex class II molecules (MHC II), but the alpha beta-dimers are targeted to late endosomes and lysosomes (often referred to as MHC class II compartments) where they reside unproductively with internalized antigens. After exposure to microbial products or inflammatory mediators, endocytosis is downregulated, the expression of co-stimulatory molecules is enhanced, and newly formed immunogenic MHC II-peptide complexes are transported to the cell surface. That these MHC II molecules reach the surface is surprising, as the lysosomes comprise the terminal degradative compartment of the endocytic pathway from which exogenous components generally cannot be recovered intact. Here we have visualized this pathway in live dendritic cells by video microscopy, using cells expressing MHC II tagged with green fluorescent protein (GFP). We show that on stimulation, dendritic cells generate tubules from lysosomal compartments that go on to fuse directly with the plasma membrane.

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Research works printed but not linked: 29

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Metadata

Authors
Chow, A., Toomre, D., Garrett, W., & Mellman, I.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2002-08
Volume
418
Issue
6901
Pages
988-994
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature01006
Cited by
375
References
1
Language
en
Licence
www.springer.com/tdm (tdm)

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