Cited by 313Immune Response and InflammationImmunotherapy and Immune ResponsesInflammasome and immune disorders

Endosomes are specialized platforms for bacterial sensing and NOD2 signalling

Nakamura, Norihiro, Lill, Jennie R., Phung, Qui, Jiang, Zhaoshi, Bakalarski, Corey, de Mazière, Ann, Klumperman, Judith, Schlatter, Megan +2 more·2014·Nature

Abstract

The detection of microbial pathogens involves the recognition of conserved microbial components by host cell sensors such as Toll-like receptors (TLRs) and NOD-like receptors (NLRs). TLRs are membrane receptors that survey the extracellular environment for microbial infections, whereas NLRs are cytosolic complexes that detect microbial products that reach the cytosol. Upon detection, both sensor classes trigger innate inflammatory responses and allow the engagement of adaptive immunity. Endo-lysosomes are the entry sites for a variety of pathogens, and therefore the sites at which the immune system first senses their presence. Pathogens internalized by endocytosis are well known to activate TLRs 3 and 7-9 that are localized to endocytic compartments and detect ligands present in the endosomal lumen. Internalized pathogens also activate sensors in the cytosol such as NOD1 and NOD2 (ref. 2), indicating that endosomes also provide for the translocation of bacterial components across the endosomal membrane. Despite the fact that NOD2 is well understood to have a key role in regulating innate immune responses and that mutations at the NOD2 locus are a common risk factor in inflammatory bowel disease and possibly other chronic inflammatory states, little is known about how its ligands escape from endosomes. Here we show that two endo-lysosomal peptide transporters, SLC15A3 and SLC15A4, are preferentially expressed by dendritic cells, especially after TLR stimulation. The transporters mediate the egress of bacterially derived components, such as the NOD2 cognate ligand muramyl dipeptide (MDP), and are selectively required for NOD2 responses to endosomally derived MDP. Enhanced expression of the transporters also generates endosomal membrane tubules characteristic of dendritic cells, which further enhanced the NOD2-dependent response to MDP. Finally, sensing required the recruitment of NOD2 and its effector kinase RIPK2 (refs 8, 9) to the endosomal membrane, possibly by forming a complex with SLC15A3 or SLC15A4. Thus, dendritic cell endosomes are specialized platforms for both the lumenal and cytosolic sensing of pathogens.

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Crossref · 10.1038/nature13133

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  • Oligopeptide transporters encoded by SLC15A3 and SLC15A4 were reported to be responsible for transporting small peptidoglycan fragments into macrophages.

    “Previously, oligopeptide transporters encoded by SLC15A3 and SLC15A4 were reported to be responsible for transporting small peptidoglycan (PGN) fragments into macrophages (Nakamura et al., 2014).”
  • Oligopeptide transporters encoded by SLC15A3 and SLC15A4 are responsible for transporting small PGN fragments into macrophages.

    “It was previously reported that oligopeptide transporters encoded by SLC15A3 and SLC15A4 are responsible for transporting small PGN fragments into macrophages (Nakamura et al., 2014).”

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Authors
Nakamura, N., Lill, J. R., Phung, Q., Jiang, Z., Bakalarski, C., de Mazière, A., Klumperman, J., Schlatter, M., Delamarre, L., & Mellman, I.
Affiliations
  • Genentech (US)
  • University Medical Center Utrecht (NL)
Corresponding author
Mellman, Ira
Venue
Nature
Published
2014-03-30
Volume
509
Issue
7499
Pages
240-244
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
OpenAlex
W2031113436
Cited by
313
References
40
Citations by year
2026: 13 · 2025: 22 · 2024: 18 · 2023: 33 · 2022: 29 · 2021: 27 · 2020: 23 · 2019: 23 · 2018: 32 · 2017: 30 · 2016: 25 · 2015: 21 · 2014: 16 · 2013: 1
FWCI
14.15
Type
journal article
Language
en
Open access
closed
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Immune Response and Inflammation
  • Life Sciences › Immunology and Microbiology › Immunology › Immunotherapy and Immune Responses
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Inflammasome and immune disorders
Keywords
signaling, endosomes, SLC15A4, dendritic cells, muramyl dipeptide, NOD-like receptors, NOD1, innate immunity, endosomal escape, TLR stimulation, inflammatory bowel disease, endocytosis, pattern recognition receptors
MeSH terms
Acetylmuramyl-Alanyl-Isoglutamine, Animals, Carrier Proteins, Cytoplasm, Dendritic Cells, Immunity, Innate, Inflammation, Ligands, Lysosomes, Nerve Tissue Proteins, Phagosomes, Endosomes, Salmonella typhimurium, Inflammatory Bowel Diseases, Membrane Transport Proteins, Mice, Nod2 Signaling Adaptor Protein

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