Molecular mechanisms of kinetochore capture by spindle microtubules

Tanaka, Kozo; Mukae, Naomi; Dewar, Hilary; van Breugel, Mark; James, Euan K.; Prescott, Alan R.; Antony, Claude; Tanaka, Tomoyuki U.

doi:10.1038/nature03483

Cited by 281Microtubule and mitosis dynamicsGenomics and Chromatin DynamicsDNA Repair Mechanisms

Molecular mechanisms of kinetochore capture by spindle microtubules

Tanaka, Kozo, Mukae, Naomi, Dewar, Hilary, van Breugel, Mark, James, Euan K., Prescott, Alan R., Antony, Claude, Tanaka, Tomoyuki U.·2005·Nature

DOI 10.1038/nature03483

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Abstract

For high-fidelity chromosome segregation, kinetochores must be properly captured by spindle microtubules, but the mechanisms underlying initial kinetochore capture have remained elusive. Here we visualized individual kinetochore-microtubule interactions in Saccharomyces cerevisiae by regulating the activity of a centromere. Kinetochores are captured by the side of microtubules extending from spindle poles, and are subsequently transported poleward along them. The microtubule extension from spindle poles requires microtubule plus-end-tracking proteins and the Ran GDP/GTP exchange factor. Distinct kinetochore components are used for kinetochore capture by microtubules and for ensuring subsequent sister kinetochore bi-orientation on the spindle. Kar3, a kinesin-14 family member, is one of the regulators that promote transport of captured kinetochores along microtubules. During such transport, kinetochores ensure that they do not slide off their associated microtubules by facilitating the conversion of microtubule dynamics from shrinkage to growth at the plus ends. This conversion is promoted by the transport of Stu2 from the captured kinetochores to the plus ends of microtubules.

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Kar3 is a well studied minus end-directed motor and contributes to mitotic processes.
“which is a well studied minus end-directed motor and also contributes to mitotic processes (17,18).”
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Authors: Tanaka, K., Mukae, N., Dewar, H., van Breugel, M., James, E. K., Prescott, A. R., Antony, C., & Tanaka, T. U.
Affiliations: University of Dundee (GB)
European Molecular Biology Laboratory (DE)
Corresponding author: Tanaka, Tomoyuki U.
ORCID: Tanaka, Kozo
James, Euan K.
Prescott, Alan R.
Tanaka, Tomoyuki U.
Venue: Nature
Publisher: Springer Science and Business Media LLC
Published: 2005-04
Volume: 434
Issue: 7036
Pages: 987-994
ISSN: 0028-0836 (ISSN-L)
1476-4687
DOI: 10.1038/nature03483
OpenAlex: W2120623923
Cited by: 281
References: 50
Citations by year: 2026: 12 · 2025: 2 · 2024: 5 · 2023: 10 · 2022: 7 · 2021: 10 · 2020: 5 · 2019: 12 · 2018: 15 · 2017: 11 · 2016: 14 · 2015: 11 · 2014: 9 · 2013: 12 · 2012: 18
FWCI: 10.82
Type: journal article
Language: en
Open access: closed
Licence: www.springer.com/tdm (tdm)
Locations: Nature (publishedVersion)
PubMed (publishedVersion)
RePEc: Research Papers in Economics (submittedVersion)
Discovery Research Portal (University of Dundee) (submittedVersion)
Max Planck Institute for Plasma Physics (submittedVersion)
MPG.PuRe (Max Planck Society) (submittedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
Keywords: spindle microtubules, chromosome segregation, kinetochore-microtubule interaction, microtubule plus-end tracking, Ran GTPase, kinesin-14, microtubule dynamics, centromere, Saccharomyces cerevisiae
MeSH terms: Biological Transport, Cell Cycle, Microtubule-Associated Proteins, Microtubules, Spindle Apparatus, Saccharomyces cerevisiae, Chromosomes, Fungal, Kinesins, Kinetochores, Chromosome Segregation, ran GTP-Binding Protein, Saccharomyces cerevisiae Proteins

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Molecular mechanisms of kinetochore capture by spindle microtubules

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