KAR3, a kinesin-related gene required for yeast nuclear fusion

Meluh, P

doi:10.1016/0092-8674(90)90351-e

Cited by 503Microtubule and mitosis dynamicsFungal and yeast genetics researchNuclear Structure and Function

KAR3, a kinesin-related gene required for yeast nuclear fusion

Meluh, P·1990·Cell

DOI 10.1016/0092-8674(90)90351-e

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Abstract

The KAR3 gene is essential for yeast nuclear fusion during mating, and its expression is strongly induced by alpha factor. The predicted KAR3 protein sequence contains two globular domains separated by an alpha-helical coiled coil. The carboxy-terminal domain is homologous to the amino-terminal mechanochemical domain of Drosophila kinesin heavy chain. Mutation of the putative ATP binding site produces a dominant "poison" of nuclear fusion. The mutant protein shows enhanced microtubule association in vivo, as predicted for a kinesin-like protein in a state of rigor binding. Localization of hybrid proteins to cytoplasmic microtubules in shmoos indicates that the amino-terminal domain also contains determinants for microtubule association. Thus, KAR3 is a member of a larger family of kinesin-like proteins characterized by the presence of the mechanochemical domain tethered to different protein binding domains. The phenotypes of kar3 mutants suggest that the protein mediates microtubule sliding during nuclear fusion and possibly mitosis.

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Authors: Meluh, P.
Affiliations: Princeton University (US)
Corresponding author: Meluh, P
ORCID: Meluh, P
Venue: Cell
Publisher: Elsevier BV
Published: 1990-03-23
Volume: 60
Issue: 6
Pages: 1029-1041
ISSN: 0092-8674 (ISSN-L)
DOI: 10.1016/0092-8674(90)90351-e
OpenAlex: W2003771584
Cited by: 503
References: 68
Citations by year: 2026: 1 · 2025: 3 · 2024: 1 · 2023: 4 · 2022: 3 · 2021: 2 · 2020: 2 · 2019: 3 · 2018: 5 · 2017: 3 · 2016: 5 · 2015: 10 · 2014: 6 · 2013: 9 · 2012: 13
FWCI: 23.75
Type: journal article
Language: en
Open access: closed
Licence: www.elsevier.com/tdm/userlicense/1.0/ (tdm)
Locations: Cell (publishedVersion)
PubMed (publishedVersion)
Funders: National Science Foundation
National Institutes of Health
National Institute of General Medical Sciences (GM 37739, R01GM037739)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords: kinesin-like protein, nuclear fusion, mating, alpha-factor, yeast, microtubule sliding, ATP binding site, coiled coil, mitosis
MeSH terms: Adenosine Triphosphatases, Adenosine Triphosphate, Amino Acid Sequence, Base Sequence, Binding Sites, Cell Nucleus, Fungal Proteins, Genes, Fungal, Genetic Complementation Test, Genotype, Microtubule-Associated Proteins, Molecular Sequence Data, Mutation, Phenotype, Plasmids, Protein Conformation, RNA, Messenger, Saccharomyces cerevisiae, Sequence Homology, Nucleic Acid, Restriction Mapping, Kinesins, Saccharomyces cerevisiae Proteins

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Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

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KAR3, a kinesin-related gene required for yeast nuclear fusion

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