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Centrosome polarization delivers secretory granules to the immunological synapse

Stinchcombe, Jane C., Majorovits, Endre, Bossi, Giovanna, Fuller, Stephen, Griffiths, Gillian M.·2006·Nature
DOI 10.1038/nature05071
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Abstract

Cytotoxic T lymphocytes (CTLs) destroy virally infected and tumorigenic cells by releasing the contents of specialized secretory lysosomes--termed 'lytic granules'--at the immunological synapse formed between the CTL and the target. On contact with the target cell, the microtubule organizing centre of the CTL polarizes towards the target and granules move along microtubules in a minus-end direction towards the polarized microtubule organizing centre. However, the final steps of secretion have remained unclear. Here we show that CTLs do not require actin or plus-end microtubule motors for secretion, but instead the centrosome moves to and contacts the plasma membrane at the central supramolecular activation cluster of the immunological synapse. Actin and IQGAP1 are cleared away from the synapse, and granules are delivered directly to the plasma membrane. These data show that CTLs use a previously unreported mechanism for delivering secretory granules to the immunological synapse, with granule secretion controlled by centrosome delivery to the plasma membrane.

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Crossref · 10.1038/nature05071

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  • Lysosome retrograde transport toward the immunological synapse is essential for natural killer and cytotoxic T lymphocyte killing of pathogen-infected cells.

    “Lysosome retrograde transport toward the immunological synapse is essential for the ability of natural killer and cytotoxic T lymphocytes to kill pathogen infected cells15”

    From TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes (2017) · cites this paper as [15]

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Research works printed but not linked: 23

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Metadata

Authors
Stinchcombe, J. C., Majorovits, E., Bossi, G., Fuller, S., & Griffiths, G. M.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2006-09-28
Volume
443
Issue
7110
Pages
462-465
ISSN
  • 0028-0836
  • 1476-4687
DOI
10.1038/nature05071
Cited by
599
References
0
Language
en
Update notices
erratum (2006-11-09)
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