The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
Open accessAbstract
Mechanical forces are central to developmental, physiological and pathological processes. However, limited understanding of force transmission within sub-cellular structures is a major obstacle to unravelling molecular mechanisms. Here we describe the development of a calibrated biosensor that measures forces across specific proteins in cells with piconewton (pN) sensitivity, as demonstrated by single molecule fluorescence force spectroscopy. The method is applied to vinculin, a protein that connects integrins to actin filaments and whose recruitment to focal adhesions (FAs) is force-dependent. We show that tension across vinculin in stable FAs is ∼2.5 pN and that vinculin recruitment to FAs and force transmission across vinculin are regulated separately. Highest tension across vinculin is associated with adhesion assembly and enlargement. Conversely, vinculin is under low force in disassembling or sliding FAs at the trailing edge of migrating cells. Furthermore, vinculin is required for stabilizing adhesions under force. Together, these data reveal that FA stabilization under force requires both vinculin recruitment and force transmission, and that, surprisingly, these processes can be controlled independently.
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Balaban, N. Q., Schwarz, U. S., Riveline, D., Goichberg, P., Tzur, G., Sabanay, I., Mahalu, D., Safran, S., Bershadsky, A., Addadi, L., et al. (2001) · Nature Cell Biology · doi:10.1038/35074532 · Cited by 2,132
Cells lying on a bed of microneedles: An approach to isolate mechanical force
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Self-citationFocal Contacts as Mechanosensors
Riveline, D., Zamir, E., Balaban, N. Q., Schwarz, U. S., Ishizaki, T., Narumiya, S., Kam, Z., Geiger, B., & Bershadsky, A. D. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.6.1175 · Cited by 1,419
Vinculin controls focal adhesion formation by direct interactions with talin and actin
Humphries, J. D., Wang, P., Streuli, C., Geiger, B., Humphries, M. J., & Ballestrem, C. (2007) · The Journal of Cell Biology · doi:10.1083/jcb.200703036 · Cited by 909
The relationship between force and focal complex development
Galbraith, C. G., Yamada, K. M., & Sheetz, M. P. (2002) · The Journal of Cell Biology · doi:10.1083/jcb.200204153 · Cited by 908
Adhesion-Dependent Cell Mechanosensitivity
Bershadsky, A. D., Balaban, N. Q., & Geiger, B. (2003) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.19.111301.153011 · Cited by 874
Spatiotemporal dynamics of RhoA activity in migrating cells
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Mechanisms of Mechanotransduction
Orr, A. W., Helmke, B. P., Blackman, B. R., & Schwartz, M. A. (2006) · Developmental Cell · doi:10.1016/j.devcel.2005.12.006 · Cited by 798
Self-citationVinculin knockout results in heart and brain defects during embryonic development
Xu, W., Baribault, H., & Adamson, E. D. (1998) · Development · doi:10.1242/dev.125.2.327 · Cited by 532
Differential Transmission of Actin Motion Within Focal Adhesions
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Metadata
- Authors
- Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T., & Schwartz, M. A.
- Venue
- Nature
- Published
- 2010-07
- Volume
- 466
- Issue
- 7303
- Pages
- 263-266
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2048305477
- Cited by
- 1,541
- References
- 30
- FWCI
- 32.53
- Type
- journal article
- Language
- en
- Open access
- green
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- Grashoff, C., Hoffman, B. D., Brenner, M. D., Zhou, R., Parsons, M., Yang, M. T., McLean, M. A., Sligar, S. G., Chen, C. S., Ha, T., & Schwartz, M. A. (2010). Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics. Nature. https://doi.org/10.1038/nature09198
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