The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Extensive transcriptional heterogeneity revealed by isoform profiling
Open accessAbstract
Transcript function is determined by sequence elements arranged on an individual RNA molecule. Variation in transcripts can affect messenger RNA stability, localization and translation, or produce truncated proteins that differ in localization or function. Given the existence of overlapping, variable transcript isoforms, determining the functional impact of the transcriptome requires identification of full-length transcripts, rather than just the genomic regions that are transcribed. Here, by jointly determining both transcript ends for millions of RNA molecules, we reveal an extensive layer of isoform diversity previously hidden among overlapping RNA molecules. Variation in transcript boundaries seems to be the rule rather than the exception, even within a single population of yeast cells. Over 26 major transcript isoforms per protein-coding gene were expressed in yeast. Hundreds of short coding RNAs and truncated versions of proteins are concomitantly encoded by alternative transcript isoforms, increasing protein diversity. In addition, approximately 70% of genes express alternative isoforms that vary in post-transcriptional regulatory elements, and tandem genes frequently produce overlapping or even bicistronic transcripts. This extensive transcript diversity is generated by a relatively simple eukaryotic genome with limited splicing, and within a genetically homogeneous population of cells. Our findings have implications for genome compaction, evolution and phenotypic diversity between single cells. These data also indicate that isoform diversity as well as RNA abundance should be considered when assessing the functional repertoire of genomes.
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Self-citationMechanisms and Consequences of Alternative Polyadenylation
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Cited by
Single-cell RNA-seq: advances and future challenges
Saliba, A.-E., Westermann, A. J., Gorski, S. A., & Vogel, J. (2014) · Nucleic Acids Research · doi:10.1093/nar/gku555 · Cited by 847
Ribosome profiling: new views of translation, from single codons to genome scale
Ingolia, N. T. (2014) · Nature Reviews Genetics · doi:10.1038/nrg3645 · Cited by 632
Schep, A. N., Buenrostro, J. D., Denny, S. K., Schwartz, K., Sherlock, G., & Greenleaf, W. J. (2015) · Genome Research · doi:10.1101/gr.192294.115 · Cited by 375
Widespread Co-translational RNA Decay Reveals Ribosome Dynamics
Pelechano, V., Wei, W., & Steinmetz, L. M. (2015) · Cell · doi:10.1016/j.cell.2015.05.008 · Cited by 324
Self-citationReyes, A., & Huber, W. (2018) · Nucleic Acids Research · doi:10.1093/nar/gkx1165 · Cited by 290
Metadata
- Authors
- Pelechano, V., Wei, W., & Steinmetz, L. M.
- Venue
- Nature
- Published
- 2013-04-24
- Volume
- 497
- Issue
- 7447
- Pages
- 127-131
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2085567058
- Cited by
- 451
- References
- 26
- FWCI
- 16.68
- Type
- journal article
- Language
- en
- Open access
- green
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