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Cited by 351Microbial Metabolic Engineering and BioproductionBiofuel production and bioconversionAlgal biology and biofuel production

The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.

Efficient ethanol production from brown macroalgae sugars by a synthetic yeast platform

Enquist-Newman, Maria, Faust, Ann Marie E., Bravo, Daniel D., Santos, Christine Nicole S., Raisner, Ryan M., Hanel, Arthur, Sarvabhowman, Preethi, Le, Chi, Regitsky, Drew D., Cooper, Susan R., Peereboom, Lars, Clark, Alana, Martinez, Yessica, Goldsmith, Joshua, Cho, Min Y., Donohoue, Paul D., Luo, Lily, Lamberson, Brigit, Tamrakar, Pramila, Kim, Edward J., Villari, Jeffrey L., Gill, Avinash, Tripathi, Shital A., Karamchedu, Padma, Paredes, Carlos J., Rajgarhia, Vineet, Kotlar, Hans Kristian, Bailey, Richard B., Miller, Dennis J., Ohler, Nicholas L., Swimmer, Candace, Yoshikuni, Yasuo·2014·Nature
DOI 10.1038/nature12771
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Abstract

The increasing demands placed on natural resources for fuel and food production require that we explore the use of efficient, sustainable feedstocks such as brown macroalgae. The full potential of brown macroalgae as feedstocks for commercial-scale fuel ethanol production, however, requires extensive re-engineering of the alginate and mannitol catabolic pathways in the standard industrial microbe Saccharomyces cerevisiae. Here we present the discovery of an alginate monomer (4-deoxy-L-erythro-5-hexoseulose uronate, or DEHU) transporter from the alginolytic eukaryote Asteromyces cruciatus. The genomic integration and overexpression of the gene encoding this transporter, together with the necessary bacterial alginate and deregulated native mannitol catabolism genes, conferred the ability of an S. cerevisiae strain to efficiently metabolize DEHU and mannitol. When this platform was further adapted to grow on mannitol and DEHU under anaerobic conditions, it was capable of ethanol fermentation from mannitol and DEHU, achieving titres of 4.6% (v/v) (36.2 g l(-1)) and yields up to 83% of the maximum theoretical yield from consumed sugars. These results show that all major sugars in brown macroalgae can be used as feedstocks for biofuels and value-added renewable chemicals in a manner that is comparable to traditional arable-land-based feedstocks.

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Crossref · 10.1038/nature12771

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Metadata

Authors
Enquist-Newman, M., Faust, A. M. E., Bravo, D. D., Santos, C. N. S., Raisner, R. M., Hanel, A., Sarvabhowman, P., Le, C., Regitsky, D. D., Cooper, S. R., Peereboom, L., Clark, A., Martinez, Y., Goldsmith, J., Cho, M. Y., Donohoue, P. D., Luo, L., Lamberson, B., Tamrakar, P., Kim, E. J., Villari, J. L., Gill, A., Tripathi, S. A., Karamchedu, P., Paredes, C. J., Rajgarhia, V., Kotlar, H. K., Bailey, R. B., Miller, D. J., Ohler, N. L., Swimmer, C., & Yoshikuni, Y.
Affiliations
  • Manus Bio (United States) (US)
  • Michigan State University (US)
  • Equinor (Norway) (NO)
Corresponding author
  • Swimmer, Candace
  • Yoshikuni, Yasuo
ORCID
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2013-12-01
Volume
505
Issue
7482
Pages
239-243
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature12771
OpenAlex
W2018440937
Cited by
351
References
30
Citations by year
2026: 10 · 2025: 15 · 2024: 16 · 2023: 25 · 2022: 28 · 2021: 35 · 2020: 29 · 2019: 35 · 2018: 28 · 2017: 22 · 2016: 43 · 2015: 40 · 2014: 25
FWCI
16.11
Type
journal article
Language
en
Open access
closed
Licence
www.springer.com/tdm (tdm)
Locations
  • Nature (publishedVersion)
  • PubMed (publishedVersion)
  • RePEc: Research Papers in Economics (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › Algal biology and biofuel production
Keywords
brown macroalgae, alcoholic fermentation, Saccharomyces cerevisiae, biofuel feedstock, renewable chemicals, glucan, yeast strain engineering, anaerobic fermentation, metabolic engineering, chromosomal integration, overexpression
MeSH terms
Ethanol, Phaeophyceae, Alginates, Anaerobiosis, Ascomycota, Biotechnology, Carrier Proteins, Fermentation, Genetic Complementation Test, Genetic Engineering, Hexuronic Acids, Mannitol, Quinic Acid, Saccharomyces cerevisiae, Seaweed, Uronic Acids, Reproducibility of Results, Evolution, Molecular, Glucuronic Acid, Carbohydrate Metabolism, Biofuels

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