The DOI matches its Crossref record; 31 of the 31 references it cites were found in a registry or an index.
Direct recording and molecular identification of the calcium channel of primary cilia
Open accessAbstract
A primary cilium is a solitary, slender, non-motile protuberance of structured microtubules (9+0) enclosed by plasma membrane. Housing components of the cell division apparatus between cell divisions, primary cilia also serve as specialized compartments for calcium signalling and hedgehog signalling pathways. Specialized sensory cilia such as retinal photoreceptors and olfactory cilia use diverse ion channels. An ion current has been measured from primary cilia of kidney cells, but the responsible genes have not been identified. The polycystin proteins (PC and PKD), identified in linkage studies of polycystic kidney disease, are candidate channels divided into two structural classes: 11-transmembrane proteins (PKD1, PKD1L1 and PKD1L2) remarkable for a large extracellular amino terminus of putative cell adhesion domains and a G-protein-coupled receptor proteolytic site, and the 6-transmembrane channel proteins (PKD2, PKD2L1 and PKD2L2; TRPPs). Evidence indicates that the PKD1 proteins associate with the PKD2 proteins via coiled-coil domains. Here we use a transgenic mouse in which only cilia express a fluorophore and use it to record directly from primary cilia, and demonstrate that PKD1L1 and PKD2L1 form ion channels at high densities in several cell types. In conjunction with an accompanying manuscript, we show that the PKD1L1–PKD2L1 heteromeric channel establishes the cilia as a unique calcium compartment within cells that modulates established hedgehog pathways.
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Cites
Ion Channels/Excitable Membranes
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ANKTM1, a TRP-like Channel Expressed in Nociceptive Neurons, Is Activated by Cold Temperatures
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Primary cilia are specialized calcium signalling organelles
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Self-citation
Cited by
Mechanically Activated Ion Channels
Ranade, S. S., Syeda, R., & Patapoutian, A. (2015) · Neuron · doi:10.1016/j.neuron.2015.08.032 · Cited by 652
Proteomics of Primary Cilia by Proximity Labeling
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Establishing and regulating the composition of cilia for signal transduction
Nachury, M. V., & Mick, D. U. (2019) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-019-0116-4 · Cited by 397
Primary Cilia in the Developing and Mature Brain
Guemez-Gamboa, A., Coufal, N. G., & Gleeson, J. G. (2014) · Neuron · doi:10.1016/j.neuron.2014.04.024 · Cited by 296
Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched
Zhang, Y., Bulkley, D. P., Xin, Y., Roberts, K. J., Asarnow, D. E., Sharma, A., Myers, B. R., Cho, W., Cheng, Y., & Beachy, P. A. (2018) · Cell · doi:10.1016/j.cell.2018.10.026 · Cited by 239
Metadata
- Authors
- DeCaen, P. G., Delling, M., Vien, T. N., & Clapham, D. E.
- Venue
- Nature
- Published
- 2013-12-11
- Volume
- 504
- Issue
- 7479
- Pages
- 315-318
- ISSN
- 0028-0836 (ISSN-L)
- 1476-4687
- OpenAlex
- W2100465045
- Cited by
- 306
- References
- 31
- FWCI
- 23.06
- Type
- journal article
- Language
- en
- Open access
- green
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