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Piezo proteins are pore-forming subunits of mechanically activated channels

Open access
Coste, Bertrand, Xiao, Bailong, Santos, Jose S., Syeda, Ruhma, Grandl, Jörg, Spencer, Kathryn S., Kim, Sung Eun, Schmidt, Manuela, Mathur, Jayanti, Dubin, Adrienne E., Montal, Mauricio, Patapoutian, Ardem·2012·Nature
DOI 10.1038/nature10812
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Abstract

Mechanotransduction has an important role in physiology. Biological processes including sensing touch and sound waves require as-yet-unidentified cation channels that detect pressure. Mouse Piezo1 (MmPiezo1) and MmPiezo2 (also called Fam38a and Fam38b, respectively) induce mechanically activated cationic currents in cells; however, it is unknown whether Piezo proteins are pore-forming ion channels or modulate ion channels. Here we show that Drosophila melanogaster Piezo (DmPiezo, also called CG8486) also induces mechanically activated currents in cells, but through channels with remarkably distinct pore properties including sensitivity to the pore blocker ruthenium red and single channel conductances. MmPiezo1 assembles as a ∼1.2-million-dalton homo-oligomer, with no evidence of other proteins in this complex. Purified MmPiezo1 reconstituted into asymmetric lipid bilayers and liposomes forms ruthenium-red-sensitive ion channels. These data demonstrate that Piezo proteins are an evolutionarily conserved ion channel family involved in mechanotransduction.

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Metadata

Authors
Coste, B., Xiao, B., Santos, J. S., Syeda, R., Grandl, J., Spencer, K. S., Kim, S. E., Schmidt, M., Mathur, J., Dubin, A. E., Montal, M., & Patapoutian, A.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2012-02-19
Volume
483
Issue
7388
Pages
176-181
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature10812
OpenAlex
W2166599924
Cited by
1,167
References
2
FWCI
33.17
Type
journal article
Language
en
Open access
green
Licence
www.springer.com/tdm (tdm)

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CoChat. (2026). Citation check: Piezo proteins are pore-forming subunits of mechanically activated channels. https://app.cochat.ai/papers/10.1038/nature10812
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Coste, B., Xiao, B., Santos, J. S., Syeda, R., Grandl, J., Spencer, K. S., Kim, S. E., Schmidt, M., Mathur, J., Dubin, A. E., Montal, M., & Patapoutian, A. (2012). Piezo proteins are pore-forming subunits of mechanically activated channels. Nature. https://doi.org/10.1038/nature10812

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