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Cited by 1,408

The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.

Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts

Open access
Chong, James J. H., Yang, Xiulan, Don, Creighton W., Minami, Elina, Liu, Yen-Wen, Weyers, Jill J., Mahoney, William M., Van Biber, Benjamin, Cook, Savannah M., Palpant, Nathan J., Gantz, Jay A., Fugate, James A., Muskheli, Veronica, Gough, G. Michael, Vogel, Keith W., Astley, Cliff A., Hotchkiss, Charlotte E., Baldessari, Audrey, Pabon, Lil, Reinecke, Hans, Gill, Edward A., Nelson, Veronica, Kiem, Hans-Peter, Laflamme, Michael A., Murry, Charles E.·2014·Nature
DOI 10.1038/nature13233
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Abstract

Pluripotent stem cells provide a potential solution to current epidemic rates of heart failure by providing human cardiomyocytes to support heart regeneration. Studies of human embryonic-stem-cell-derived cardiomyocytes (hESC-CMs) in small-animal models have shown favourable effects of this treatment. However, it remains unknown whether clinical-scale hESC-CM transplantation is feasible, safe or can provide sufficient myocardial regeneration. Here we show that hESC-CMs can be produced at a clinical scale (more than one billion cells per batch) and cryopreserved with good viability. Using a non-human primate model of myocardial ischaemia followed by reperfusion, we show that cryopreservation and intra-myocardial delivery of one billion hESC-CMs generates extensive remuscularization of the infarcted heart. The hESC-CMs showed progressive but incomplete maturation over a 3-month period. Grafts were perfused by host vasculature, and electromechanical junctions between graft and host myocytes were present within 2 weeks of engraftment. Importantly, grafts showed regular calcium transients that were synchronized to the host electrocardiogram, indicating electromechanical coupling. In contrast to small-animal models, non-fatal ventricular arrhythmias were observed in hESC-CM-engrafted primates. Thus, hESC-CMs can remuscularize substantial amounts of the infarcted monkey heart. Comparable remuscularization of a human heart should be possible, but potential arrhythmic complications need to be overcome.

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Metadata

Authors
Chong, J. J. H., Yang, X., Don, C. W., Minami, E., Liu, Y.-W., Weyers, J. J., Mahoney, W. M., Van Biber, B., Cook, S. M., Palpant, N. J., Gantz, J. A., Fugate, J. A., Muskheli, V., Gough, G. M., Vogel, K. W., Astley, C. A., Hotchkiss, C. E., Baldessari, A., Pabon, L., Reinecke, H., Gill, E. A., Nelson, V., Kiem, H.-P., Laflamme, M. A., & Murry, C. E.
Venue
Nature
Publisher
Springer Science and Business Media LLC
Published
2014-04-30
Volume
510
Issue
7504
Pages
273-277
ISSN
  • 0028-0836 (ISSN-L)
  • 1476-4687
DOI
10.1038/nature13233
OpenAlex
W2086294280
Cited by
1,408
References
2
FWCI
61.93
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts. https://app.cochat.ai/papers/10.1038/nature13233
The paper
Chong, J. J. H., Yang, X., Don, C. W., Minami, E., Liu, Y.-W., Weyers, J. J., Mahoney, W. M., Van Biber, B., Cook, S. M., Palpant, N. J., Gantz, J. A., Fugate, J. A., Muskheli, V., Gough, G. M., Vogel, K. W., Astley, C. A., Hotchkiss, C. E., Baldessari, A., Pabon, L., . . . Murry, C. E. (2014). Human embryonic-stem-cell-derived cardiomyocytes regenerate non-human primate hearts. Nature. https://doi.org/10.1038/nature13233

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.